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Theoretical Analysis of Chromatographic Peak Asymmetry and Sharpness by the Moment Method Using Two Peptides 원문보기

Biotechnology and bioprocess engineering : Bbe, v.9 no.6, 2004년, pp.495 - 499  

Choi Du Young (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University) ,  Row Kyung Ho (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University)

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The analyses of peak shapes in chromatography are useful in operating chroma­tographic system. The asymmetry and sharpness of a chromatographic peak are estimated by the reversed-phase adsorption of two standard peptides (angiotensin II bradykinin) on $C_{18}$ In this work, the average pa...

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제안 방법

  • The purpose of this work was to survey the effect of the third and fourth central moment from the chromatographic elution curves of two peptides according to different particle sizes (5, 15 μm), pore sizes (100, 300 A) and flow rates (0.5, 0.75, 1.0, 1.25, 1.5 mL/min). Also, the skewness and excess, as well as the asymmetry factor were determined according to particle and pore sizes.

대상 데이터

  • In this experiment, the HPLC was performed using a Waters 600S solvent delivery system (Waters, Milford, MA, USA). A 2487 UV dual channel detector (Waters, Milford, MA, USA) was used. The data acquisition system was a Millenium52 (Waters) system installed in a HP Vectra 500 PC.
  • The molecular weights of the two peptides, anigotensin II (C50H71N13O12 xH2O) and bradykinin (C50H73N15O11) were 1, 046 and 1, 240, respectively. The HPLC grade solvent, acetonitrile, was from Ducksan Pure Chemical (Kyungki-Do, Korea). Trifluoroacetic acid (TFA) was purchased from Sigma (St.
  • A 2487 UV dual channel detector (Waters, Milford, MA, USA) was used. The data acquisition system was a Millenium52 (Waters) system installed in a HP Vectra 500 PC. The water was filtered by an ultra-pure water system (Milipore, Bedford, MA, USA).
  • The authors gratefully acknowledge the financial support of the Center of Advanced Bioseparation Technology. This work was performed in the High-Purity Separation Laboratory of Inha University located in Incheon, Korea.

이론/모형

  • The chromatographic peaks were analyzed by the method of moments [5-8]. In general, in order to completely characterize an arbitrary probability distribution function, all moments are required.
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참고문헌 (11)

  1. Pap, T. L. and Z. Papai (2001) Application of a new mathematical function for describing chromatographic peaks. J. Chromatogr. A 950: 53-60 

  2. Papai, Zs. and T. L. Pap (2002) Analysis of peak asymme-try in chromatography. J. Chromatogr. A 953: 31-38 

  3. Lee, S. K. and K. H. Row (2004) Optimum separation condition of peptides in reversed-phase liquid Chromatog-raphy. J. Chromatogr. B 800: 115-120 

  4. Lee, J. W. and K. H. Row (2001) New retention mecha-nism of mononucleotides with buffer concentrations inion-suppressing RP-HPLC. Biotechnol. Bioprocess Eng. 6:37-41 

  5. Miyabe, K. and M. Suzuki (1992) Chromatography of liq-uid-phase adsorption on octadecylsily-silica gel. AIChE J.38: 901-910 

  6. Row, K. H., C. H. Lee, and J. H. Kang (2002) Parameters estimation of perillyl alcohol in RP-HPLC by moment analysis. Biotechnol. Bioprocess Eng. 7: 16-20 

  7. Miyabe, K. and M. Suzuki (1994) Mass-transfer phenom ena on the surface of adsorbent in reversed-phase chroma tography. Ind. Eng. Chem. Res. 33: 1792-1802 

  8. Miyabe, K. and M. Suzuki (1995) Chromatographic study on liquid-phase adsorption on octadecylsilyl-silica gel. AIChE J. 41: 548-558 

  9. Kanji, M., S. Sayuri, and G. Georges (2001) Retention and mass transfer characteristics in reversed-phase liquid chromatography using a tetrahydrofuran-water solution as the mobile phase. J. Chromatogr. A 919: 231-244 

  10. Abdel, S. S. (1981) Theory and Mathematics of Chroma-tography. Verlag GmbH, Heidelberg, Germany 

  11. Gebauer, K. H., X. L. Luo, N. G. Barton, and A. N. Stokes (2003) Efficiency of preparative and process column dis-tribution systems. J. Chromatogr. A 1006: 45-60 

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