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Selection of Optimum Expression System for Production of Kringle Fragment of Human Apolipoprotein(a) in Saccharomyces cerevisiae

Biotechnology and bioprocess engineering v.9 no.6 , 2004년, pp.523 - 527  
Cha Kwang Hyun (Department of Agricultural Biotechnology and Center for Agricultural Biotechnology, Seoul National University ) ;  Kim Myoung Dong ( Department of Agricultural Biotechnology and Center for Agricultural Biotechnology, Seoul National University ) ;  Lee Tae Hee ( Department of Agricultural Biotechnology and Center for Agricultural Biotechnology, Seoul National University ) ;  Lim Hyung Kweon ( Mogam Biotechnology Research Institute ) ;  Jung Kyung Hwan ( Department of Food and Biotechnology, Chungju National University ) ;  Seo Jin Ho ( Department of Agricultural Biotechnology and Center for Agricultural Biotechnology, Seoul National University)
Abstract

Recombinant Saccharomyces cerevisiae expression systems were developed to pro­duce a novel human anti-angiogenic protein called LK8, an 86 amino-acid kringle fragment pro­tein with three disulfide linkages. Galactose-inducible LK8 expression plasmid was constructed, and LK8 production levels by four S. cerevisiae strains were compared in order to select an op­timal host strain. S. cerevisiae 2805 was the most efficient among the strains tested. Elevating the LK8 gene copy number through multiple integration using 8-sequences as target sites re­sulted in more than a two-fold increase in the LK8 production level compared with the plasmid­based expression system. The maximum LK8 protein concentration of 25 mg/L was obtained from batch cultivation of the yeast transformant that harbors 16 copies of the LK8 gene. In con­clusion, the strain integrated with the multiple LK8 gene secreted the protein with relatively high yield, although, the increased LK8 gene dosage over 11 copies did not lead to further en­hancement in batch cultivations.

주제어

#apolipoprotein(a)   #kringle   #Saccharomyces cerevisiae   #??????-integration  

저자의 다른 논문

Cha Kwang Hyun(1) Lee, Tae-Hee(7) Lim Hyung Kweon(1) Seo, Jin-Ho(85)

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이 논문을 인용한 문헌 (1)

  1. 2005. "" Biotechnology and bioprocess engineering, 10(6): 576~581 

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