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Abstract

Recombinant Saccharomyces cerevisiae expression systems were developed to pro­duce a novel human anti-angiogenic protein called LK8, an 86 amino-acid kringle fragment pro­tein with three disulfide linkages. Galactose-inducible LK8 expression plasmid was constructed, and LK8 production levels by four S. cerevisiae strains were compared in order to select an op­timal host strain. S. cerevisiae 2805 was the most efficient among the strains tested. Elevating the LK8 gene copy number through multiple integration using 8-sequences as target sites re­sulted in more than a two-fold increase in the LK8 production level compared with the plasmid­based expression system. The maximum LK8 protein concentration of 25 mg/L was obtained from batch cultivation of the yeast transformant that harbors 16 copies of the LK8 gene. In con­clusion, the strain integrated with the multiple LK8 gene secreted the protein with relatively high yield, although, the increased LK8 gene dosage over 11 copies did not lead to further en­hancement in batch cultivations.

참고문헌 (24)

  1. Carmeliet, P. and R. K. Jain (2000) Angiogenesis in cancer and other diseases. Nature 407: 249-257 
  2. Park, S. J., K. Ryu, C. W. Suh, Y. G. Chai, O. B. Kwon, S. K. Park, and E. K. Lee (2002) Solid-phase refolding of poly-lysine tagged fusion protein of hEGF and angiogenin. Biotechnol. Bioprocess Eng. 7: 1-5 
  3. Mumberg, D., R. Muller, and M. Funk (1994) Regulatable promoters of Saccharomyces cerevisiae: Comparison of transcription activity and their use for heterologous ex-pression. Nucleic Acids Res. 22: 5767-5768 
  4. van Dijken, J. R, J. Bauer, L. Brambilla, P. Duboc, J. M Francois, C. Gancedo, M. L. Giuseppin, J. J. Heijne, M. Hoare, H. C. Lange, E. A. Madden, P. Niederberger, J. Nielsen, J. L. Parou, T. Petit, D. Porro, M. Reuss, N. van Riel, M. Rizzi, H. Y. Steensma, C. T. Verrips, J. Vindelov, and J. T. Pronk (2000) An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains. Enzyme Micro. Technol. 26: 706-714 
  5. Lee, F. W. F. and N. A. Da Silva (1997) Improved effi-ciency and stability of multiple cloned gene insertions atthe S-sequences of Saccharomyces cerevisiae. Appl. Micro-biot. Biotechnol. 48: 339-345 
  6. Kim, M. D., S. K. Rhee, and J. H. Seo (2001) Enhanced production of anticoagulant hirudin in recombinant Saccharomyces cerevisiae by chromosomal -integration. J. Biotechnol. 85: 41-48 
  7. Wang, X., Z. Wang, and N. A. Da Silva (1996) G418 selection and stability of cloned genes integrated at the chromosomal -sequences of Saccharomyces cerevisiae. Biotechnol. Bioeng. 49: 45-51 
  8. Kim, J. S., H. K. Yu, J. H. Ahn, H. J. Lee, S. W. Hong, K. H. Jung, S. I. Chang, Y. K. Hong, Y. A. Joe, S. M. Byun, S. K. Lee, S. I. Chung, and Y. Yoon (2004) Human apolipoprotein(a) kringle V inhibits angiogenesis in vitro and in vivo by interfering with the activation of focal adhesion kinases. Biochem. Biophys. Res. Commun. 313: 534-540 
  9. Biemans, R., D. Thines, T. Rutgers, M. De Wilde, and T. Cabezon (1991) The large surface protein of hepatitis B virus is retained in the yeast endoplasmic reticulum and provokes its unique enlargement. DNA Cell Biol. 10: 191-200 
  10. Kim, M. D., Y. J. Yoo, S. K. Rhee, and J. H. Seo (2001) Enhanced transformation efficiency of anticoagulant hi-rudin gene into Saccharomyces cerevisiae by double B-sequence. J. Microbiot. Biotechnot. 11: 61-64 
  11. Colville-Nash, P. R. and D. A. Willoughby (1997) Growth factors in angiogenesis: Current interest and therapeutic potential. Mol. Med. Today 4: 14-23 
  12. Moehle, C. M., M. W. Aynardi, M. R., Kolodny, F. J. Park, and E. W. Jones (1987) Protease B of Saccharomyces Cere-visiae: Isolation and regulation of the PRBl structural gene. Genetics 115: 255-263 
  13. Kim, Y. S., S. Y. Kim, J. H. Kim, and S. C. Kim (1999) Xylitol production using recombinant Saccharomyces cerevisiae containing multiple xylose reductase genes at Chro-mosomal S-sequences. J. Biotechnol. 67: 159-171 
  14. Parekh, J. B. and K. D. WittruP (1997) Expression level tuning for optimal heterologous protein secretion in Saccharomyces cerevisiae. Biotechnol. Prog. 13: 117-122 
  15. Sohn, I. H., S. K. Lee, E. S. Choi, and S. K. Rhee (1991) Gene expression and secretion of the anticoagulant hirudin in Saccharomyces cerevisiae. J. Microbiol Biotechnol, 1:266-273 
  16. Lim, Y. Y., S. M. Park, Y. S. Jang, M. S. Yang, and D. H. Kim (2003) Production of a functional mouse interferon from recombinant Saccharomyces cerevisiae. J. Microbiol. Biotechnol. 13: 537-543 
  17. Kim, H. J., J. N. Park, H. O. Kim, D. J. Shin, J. E. Chin, H. B. Lee, S. B. Chun, and S. Bai (2002) Cloning and expression of Paenibacillus sp. neopullunase gene in Saccharomyces cerevisiae producing Schwanniomyces occidentalis glucoamylase. J. Microbiol. Biotechnol. 12: 340-344. 
  18. Cho, K. M., Y. J. Yoo, and H. S. Kang (1999) -integ-ration of endo/exo-glucanase and -glucosidase genes into the yeast chromosomes for direct conversion of cellulose to ethanol. Enzyme Microb. Technol. 25: 23-30 
  19. Peterson M. S, M. D. Kim, K. C. Han, J. H. Kim, and J.H. Seo (2002) Flow cytometric analysis of human Iy-sozyme production in recombinant Saccharomyces Cere-visiae. Biotechnol. Bioprocess Eng. 7: 52-55 
  20. Brower, V (1999) Tumor angiogenesis : New drugs on the block. Nat. Biotechnol. 17: 963-968 
  21. Sambrook, J. and D. W. Russell (2001) Molecutar Clon-ing: A Laboratory Manual. 3rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y., USA 
  22. Brachmann C. B., A, Davis, G. J. Cost, E. Caputo, P. Hieter, and J. D. Boeke (1998) Designer deletion strains derived from Saccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disrup-tion and other applications. Yeast 30: 115-132 
  23. Kim, M. D., K. C. Han, H. A. Kang, S. K. Rhee, and J. H. Seo (2003) Coexpression of BiP increased antithrombotichirudin production in recombinant Saccharomyces Cere-visiae. J. Biotechnol. 101: 81-87 
  24. Robinson, A. S., V. Hines, and K. D. WittruP (1994) Pro-tein disulfide isomerase overexpression increases secretion of foreign proteins in Saccharomyces cerevisiae. Biotechnol 12:381-384 

이 논문을 인용한 문헌 (1)

  1. 2005. "" Biotechnology and bioprocess engineering, 10(6): 576~581 

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