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논문 상세정보

Comparative Analysis of Intracellular Trans-Splicing Ribozyme Activity Against Hepatitis C Virus Internal Ribosome Entry Site

The journal of microbiology v.42 no.4 , 2004년, pp.361 - 364  

Internal ribosome entry site (IRES) of the hepatitis C virus (HCV) is known to be essential for HCV replication and most conserved among HCV variants. Hence, IRES RNA is a good therapeutic target for RNA-based inhibitors, such as ribozymes. We previously proposed a new anti-HCV modulation strategy based on trans-splicing ribozymes, which can selectively replace HCV transcripts with a new RNA that exerts anti-HCV activity. To explore this procedure, sites which are accessible to ribozymes in HCV IRES were previously determined by employing an RNA mapping method in vitro. In this study, we evaluate the intracellular accessibility of the ribozymes by comparing the trans-splicing activ­ities in cells of several ribozymes targeting different sites of the HCV IRES RNA. We assessed the intra­cellular activities of the ribozymes by monitoring their target-specific induction degree of both reporter gene activity and cytotoxin expression. The ribozyme capable of targeting the most accessible site iden­tified by the mapping studies then harbored the most active trans-splicing activity in cells. These results suggest that the target sites predicted to be accessible are truly the most accessible in the cells, and thus, could be applied to the development of various RNA-based anti-HCV therapies.

참고문헌 (15)

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  6. Ryu, K.-J., J.-H. Kim, and S.-W. Lee. 2003. Ribozyme-mediated selective induction of new gene activity in hepatitis C virus internal ribosome entry site-expressing cells by targeted transsplicing. Mol. Ther. 7, 386-395 
  7. Byun, J., N. Lan, M. Long, and B.A. Sullenger. 2003. Efficient and specific repair of sickle beta-globin RNA by trans-splicing ribozymes. RNA 9, 1254-1263 
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이 논문을 인용한 문헌 (3)

  1. 2006. "" The journal of microbiology, 44(1): 72~76 
  2. Kim, Ju-Hyun ; Lee, Chang-Ho ; Jang, Sun-Young ; Lee, Seong-Wook 2008. "Development of Trans-Splicing Aptazyme Which Can Specifically Modify Hepatitis C Virus Genome" Korean journal of microbiology = 미생물학회지, 44(3): 186~192 
  3. 2009. "" Genomics & informatics, 7(4): 181~186 


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