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Laccase Production Using Pleurotus ostreatus 1804 Immobilized on PUF Cubes in Batch and Packed Bed Reactors: Influence of Culture Conditions

The journal of microbiology v.43 no.3 , 2005년, pp.301 - 307  
Prasad K. Krishna (Bioengineering and Environmental Centre, Indian Institute of Chemical Technology ) ;  Mohan S. Venkata ( Bioengineering and Environmental Centre, Indian Institute of Chemical Technology ) ;  Bhaskar Y. Vijaya ( Bioengineering and Environmental Centre, Indian Institute of Chemical Technology ) ;  Ramanaiah S. V. ( Bioengineering and Environmental Centre, Indian Institute of Chemical Technology ) ;  Babu V. Lalit ( Bioengineering and Environmental Centre, Indian Institute of Chemical Technology ) ;  Pati B. R. ( Department of Microbiology, Vidyasagar University ) ;  Sarma P. N. ( Bioengineering and Environmental Centre, Indian Institute of Chemical Technology)
Abstract

The feasibility of laccase production by immobilization of Pleurotus ostreatus 1804 on polyurethane foam (PUF) cubes with respect to media composition was studied in both batch and reactor systems. Enhanced laccase yield was evidenced due to immobilization. A relatively high maximum laccase activity of 312.6 U was observed with immobilized mycelia in shake flasks compared to the maximum laccase activity of free mycelia (272.2 U). It is evident from this study that the culture conditions studied, i.e. biomass level, pH, substrate concentration, yeast extract concentration, $Cu^{2+}$ concentration, and alcohol nature, showed significant influence on the laccase yield. Gel electrophoretic analysis showed the molecular weight of the laccase produced by immobilized P. ostreatus to be 66 kDa. The laccase yield was significantly higher and more rapid in the packed bed reactor than in the shake flask experiments. A maximum laccase yield of 392.9 U was observed within 144 h of the fermentation period with complete glucose depletion.

주제어

#Laccase   #Pleurotus ostreatus 1804   #immobilization   #packed bed reactor   #culture condit conditions  

참고문헌 (19)

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이 논문을 인용한 문헌 (3)

  1. 2007. "" Journal of microbiology and biotechnology, 17(7): 1071~1078 
  2. 2007. "" Journal of microbiology and biotechnology, 17(8): 1369~1378 
  3. 2007. "" Journal of microbiology and biotechnology, 17(8): 1369~1378 

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