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제안 방법
- In order to study the effect of packed bed bioreactor con figuration on laccase production by immobilized P. ostreatus on PUF cubes studied, the investigations were performed in a laboratory scale glass reactor using the optimized production conditions obtained from previously discussed batch studies (pH 5.5; inoculum level 1.38 (wet weight of mycelia g/1); carbon source 1.5; yeast extract- 0.5%; copper 1.5 mM). During the reactor operation, ali quots of samples were withdrawn from the reactor every 24 h and analyzed for glucose consumption and laccase production.
- In this report, we made an attempt to investigate the fea sibility of immobilization of Pleurotus ostreatus 1804 on laccase production with the function of media composi tion in both batch and reactor systems.
대상 데이터
- The optimized culture conditions obtained from batch studies were fiirther studied for laccase production in a packed bed reactor. The reactor with a working volume of 280 ml was fabricated using 'BorosiP glass with an inter nal diameter of 4.5 cm and height of 25 cm (Fig. 1). The reactor was packed with PUF cubes (3x3x3 cm) placed on a porous plate fixed at the bottom, each with mycelia concentration of approximately 0.
이론/모형
- The residual glucose in the media (hiring fer mentation was measured colorimetrically (UV-Vis spec- trophotometer-Beckman DU-7400, USA) using the DNS (dinitrosalicylic acid) method with glucose as standard (Ghose, 1987). The extracellular protein concentration in the fermentation broth was estimated by Lowry's method using bovine serum albumin (BSA) as standard (Lowery, 1951). The wet weight of immobilized fungal mycelium on PUF was determined each time using separate sets of fermented flasks.
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