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최대 10 단어까지만 선택 가능합니다.
제안 방법
- In this paper, we report the integration of an ELISA into a PDMS microchip using the liquid-cord waveguide as a detector. Microcystin-LR, the most frequently found cyanobacterial toxin in aqueous environment, was assayed with this system.
- The MCLR sample determination was performed with this PDMS microchip-based ELISA system. Figure 4 indicated that the calibration curve we got correlated properly with the conventional ELISA ones.
대상 데이터
- All the solutions were pumped into the system by 4 channel peristaltic pump (Ismatec, Switzerland). The PMT data acquisition system was Autochro Data Module (Younglin Instrument, Korea). For antigen-antibody reaction, we used infuse-only dual syringe pump (Harvard Apparatus Model, USA), syringe (Norm-Ject, 1 mL) and connect tubing (TYGON® S-54-HL Microbore Tubing).
- The chip was composed of three parts (Figure 3), the transparent PDMS microchip (30 mm ×20 mm) acted as the reaction chip, the frit and the black PDMS microchip (40 mm × 20 mm) with 5 cm length of optical tubing (OD: 508 µm, ID: 254 µm), which worked as the detection chip; the thickness of both chip were about 4 mm and the height of microstructures was 100 µm. We fabricated two chips respectively as reported previously.
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