[논문]Genetic Transformation of Ajuga multiflora Bunge with Agrobacterium rhizogenes and 20-Hydroxyecdysone Production in Hairy Roots

Kim Ok Tae; Manickavasagm M.; Kim Young Jun; Jin Mei Ran; Kim Kwang Soo; Seong Nak Sul; Hwang Baik

doi:10.1007/bf03030416

Genetic Transformation of Ajuga multiflora Bunge with Agrobacterium rhizogenes and 20-Hydroxyecdysone Production in Hairy Roots

Journal of plant biology = 식물학회지, v.48 no.2, 2005년, pp.258 - 262

Kim Ok Tae (Department of Biology and Institute of Plant Resources, Chonnam National University) , Manickavasagm M. (Department of Biotechnology, Bharathidasan University) , Kim Young Jun (Department of Biology and Institute of Plant Resources, Chonnam National University) , Jin Mei Ran (Department of Biology and Institute of Plant Resources, Chonnam National University) , Kim Kwang Soo (Mokpo Experiment Station, National Institute of Crop Science, RDA) , Seong Nak Sul (National Institute of Crop Science, RDA) , Hwang Baik (Department of Biology and Institute of Plant Resources, Chonnam National University)

Abstract ▼ AI-Helper

An efficient transformation system for Ajuga multiflora Bunge was established by using Agrobacterium rhizogenes strain A4. After inoculation with the bacteria, we obtained a number of hairy-root clones from micro-calli of the explant petioles. One fast-growing line showed the highest production of 20-hydroxyecdysone (20-HE). PCR amplification of rooting locus (rol) genes revealed that the left hand-transferred DNA of the root-inducing plasmid was inserted into the genome of our transformed Ajuga hairy roots. This integration was further confirmed by DNA-DNA hybridization. The 20-HE content in hairy roots was 10 times higher than that measured in the wild type.

주제어

참고문헌 (19)

Plant Sci J Bercetche 52 195 1987 10.1016/0168-9452(87)90053-7 Bercetche J, Chriqui D, Adam S, David C (1987) Morpho-genetic and cellular reorientation induced byAgrobacterium rhizogenes (strains 1855, 2659 and 8196) on carrot, pea and tobacco. Plant Sci 52: 195-210

상세보기
Biotechnol Bioeng R Bhadra 41 581 1993 10.1002/bit.260410511 Bhadra R, Vani S, Shanks JV (1993) Production of indole alkaloids by selected hairy root lines ofCatharanthus roseus. Biotechnol Bioeng 41: 581-592

상세보기
Annu Rev Microbiol AN Binns 42 575 1988 10.1146/annurev.mi.42.100188.003043 Binns AN, Tomashow JV (1988) Cell biology ofAgrobacterium infection and transformation of plants. Annu Rev Microbiol 42: 575-606

상세보기
Phytochem F Camps 32 1361 1993 10.1016/0031-9422(93)85139-I Camps F, Coll J (1993) Insect allelochemicals fromAjuga plants. Phytochem 32: 1361-1370

상세보기
Phytochem Bull JJ Doyle 19 11 1987 Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11-15
Plasmid L Jouanin 12 91 1984 10.1016/0147-619X(84)90055-6 Jouanin L (1984) Restriction map of an agropine-type Ri plasmid and its homologies with Ti plasmids. Plasmid 12: 91-102

상세보기
J Plant Biol ML Lian 45 201 2002 10.1007/BF03030360 Lian ML, Chakrabarty D, Peak KY (2002) Effect of plant growth regulators and medium composition on cell growth and saponin production during cell-suspension culture of mountain ginseng (Panaxginseng C.A. Meyer). J Plant Biol 45: 201-206

상세보기
J Plant Biol OT Kim 47 361 2004 10.1007/BF03030552 Kim OT, Kim MY, Huh SM, Ahn JC, Seong NS, Hwang B (2004a) Effect of growth regulators on asiaticoside production in whole plant cultures ofCentella asiatica (L.) Urban. J Plant Biol 47: 361-365

원문보기 상세보기
J Plant Biol YS Kim 47 356 2004 10.1007/BF03030551 Kim YS, Hahn EJ, Murthy HN, Paek KY (2004b) Effect of polyploidy induction on biomass and ginsenoside accumulations in adventitious roots of ginseng. J Plant Biol 47: 356-360

원문보기 상세보기
In Vitro Cell Dev Biol IE Maldonado-Mendoza 28 67 1992 10.1007/BF02823021 Maldonado-Mendoza IE, Ayora-Talavera T, Loyola-Vargas VM (1992) Tropane alkaloid production inDatura stramonium root cultures. In Vitro Cell Dev Biol 28: 67-72

상세보기
Agric Biol Chem T Matsumoto 55 1019 1991 10.1271/bbb1961.55.1019 Matsumoto T, Tanaka N (1991) Production of phytoecdys-teroids by hairy root culture ofAjuga reptans var.atropurpurea. Agric Biol Chem 55: 1019-1025

상세보기
Physiol Plant T Murashige 15 473 1962 10.1111/j.1399-3054.1962.tb08052.x Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497

상세보기
Plant Cell Tiss Org Cult MP Ottani 20 25 1990 10.1007/BF00034753 Ottani MP, Schel JHN, Hanisch ten Cate Ch H (1990) Variation in structure and plant regeneration ofAgrobacte-rium rhizogenes transformed and control roots of the potato cv. Bintje. Plant Cell Tiss Org Cult 20: 25-34

상세보기
Plant Cell Rep HP Shi 21 1103 2003 10.1007/s00299-003-0633-6 Shi HP, Kintzios S (2003) Genetic transformation ofPueraria phaseoloides with Agrobacterium rhizogenes and puerarin production in hairy roots. Plant Cell Rep 21: 1103-1107

상세보기
Phytochem K Shimomura 30 2275 1991 10.1016/0031-9422(91)83629-Y Shimomura K, Sauerwein M, Ishimaru K (1991) Tropane alkaloids in the adventitious and hairy root cultures of solanaceous plants. Phytochem 30: 2275-2278

상세보기
Plant Tiss Cult Lett N Tanaka 10 78 1993 10.5511/plantbiotechnology1984.10.78 Tanaka N, Matsumoto T (1993a) Characterization ofAjuga plants regenerated from hairy roots. Plant Tiss Cult Lett 10: 78-83
Plant Cell Rep N Tanaka 13 87 1993 10.1007/BF00235296 Tanaka N, Matsumoto T (1993b) Regenerants from Ajuga hairy roots with high productivity of 20-hydroxyecdys-one. Plant Cell Rep 13: 87-90

상세보기
J Ferment Bioeng N Uozumi 80 362 1995 10.1016/0922-338X(95)94205-6 Uozumi N, Makino S, Kobayashi T (1995) 20-Hydroxy-ecdysone production inAjuga hairy root controlling intracellular phosphate based on kinetic model. J Ferment Bioeng 80: 362-368

상세보기
J Bacterid FF White 41 1134 1980 10.1128/JB.141.3.1134-1141.1980 White FF, Nester EW (1980) Hairy roots: Plasmid encodes virulence traits inAgrobacterium rhizogenes. J Bacterid 41: 1134-1141

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

AI-Helper ※ AI-Helper는 을 사용합니다.

AI-Helper

안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증