[논문]Overexpression and Characterization of appA Phytase Expressed by Recombinant Baculovirus-Infected Silkworm

CHEN YIN; ZHU ZHONGZE; LIN XU'AI; YI YONGZHU; ZHANG ZHIFANG; SHEN GUIFANG

Overexpression and Characterization of appA Phytase Expressed by Recombinant Baculovirus-Infected Silkworm 원문보기

Journal of microbiology and biotechnology, v.15 no.3, 2005년, pp.466 - 471

CHEN YIN (Sericultural Research Institute, Chinese Academy of Agricultural Sciences) , ZHU ZHONGZE (Sericultural Research Institute, Chinese Academy of Agricultural Sciences) , LIN XU'AI (Sericultural Research Institute, Chinese Academy of Agricultural Sciences) , YI YONGZHU (Sericultural Research Institute, Chinese Academy of Agricultural Sciences) , ZHANG ZHIFANG (Sericultural Research Institute, Chinese Academy of Agricultural Sciences) , SHEN GUIFANG (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences)

Abstract ▼ AI-Helper

An Escherichia coli strain with high phytase activity was screened from pig excreta. The phytase gene, appA, was amplified by PCR technique. To obtain large amounts of appA phytase, the appA gene was subcloned into the baculovirus transfer vector pVL1393 under the control of the Polyhedrin promoter. The recombinant baculovirus harboring the appA gene was obtained after co-transfection and screening. The early $5^{th}$ instar larvae of silkworm were infected with the recombinant virus. Using this system, the appA phytase was overproduced up to 7,710 U per ml hemolymph. SDS-PAGE analysis revealed the baculovirus-derived appA phytase to be approximately 47 kDa in size. The optimal temperature and pH of the expressed phytase were $60^{\circ}C$ and pH 4.5, respectively. The enzymatic activity was increased by the presence of 1 mM $Ca^{2+}$, 1 mM $Mn^{2+}$, or $0.02\%$ Triton X-100.

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