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가설 설정
- a,b Values with diffrent superscripts are significantly different (p<0.05).
제안 방법
- The purpose of this study was to investigate in vitro maturation rate of oocytes cultured in maturation medium with supplementation of EGF, 0-ME, glucose, and further development of NT embryos reconstructed with differently subcultured fibroblast cells cultured in different media and gas atmospheres.
- This study was investigated in vitro maturation rate of oocytes cultured in maturation media with or without supplementation of EGF, ME, glucose and further development of NT embryos reconstructed with differently subcultured cells cultured in different media and gas atmospheres.
데이터처리
- All data were subjected to a Generalized Linear Model procedure (PROC-GLM) of the statistical analysis system (SAS Institute, Gary, NC, USA). Differences among treatment means were determined using Duncan's multiple range test and /-test.
- All data were subjected to a Generalized Linear Model procedure (PROC-GLM) of the statistical analysis system (SAS Institute, Gary, NC, USA). Differences among treatment means were determined using Duncan's multiple range test and /-test. All the data were expressed as least square (LS) mean±S.
성능/효과
- 1. When the embryos were cultured in NCSU-23 or PZM-3 supplemented with or without 20 ng/ml EGF for 144 h, the development rates to blastocyst stage were 12.0±1.3%, 9.6±1.9 %, 10.9±2.1% and 9.1±2.3%, respectively.
- The developmental rates to 2 cell and blastocyst stage of embryos cultured in NCSU-23 or PZM-3 supplemented with or without 25 p-ME were shown in Table 2. When the embryos were cultured in NCSU-23 or PZM-3 supplemented with or without 25 "M p-ME for 144 h, the development rates to the blastocyst stage were 9.6±1.7%, 7.3±2.3%, 11.9±1.8% and 7.4±2.1%, respectively. The developmental rate to the blastocyst stage of NT embryos cultured in PZM-3 supplemented with p- ME was significantly higher cultured in none supplementation of P-ME (p<0.
- The developmental rates to 2 cell and blastocyst stage of embryos cultured in NCSU-23 or PZM-3 supplemented with or without 1.5 mM glucose were shown in Table 3. When the embryos were cultured in NCSU-23 or PZM-3 supplemented with or without 1.5 mM glucose for 144 h, the development rates to the blastocyst stage were 9.4±2.2%,6.8±2.7%, 10.9±2.4% and 8.9±2.6%, respectively. The developmental rate to the blastocyst stage of NT embryos cultured in NCSU-23 and PZM-3 supplemented with glucose was higher than cultured in none supplementation of glucose.
- 4. When NT embryos were cultured in NUSU- 23 and PZM-3 at 5% and 20% O2 concentration, the developmental rates to blastocyst stage were 11.1±1.8%, 9.8±1.4%, 12.5±1.6% and 10.9±1.5%, respectively. The developmental rate to the blastocyst stage of NT embryos cultured in both NCSU-23 and PZM- 3 at 5% O2 concentration was higher than cultured at 20% O2 concentration.
- 5. When fetal fibroblasts were cultured in NCSU- 23 and used as donor cells for NT embryos, the fusion rate of less than 10 passage was higher than those of 11~15 passage (60.0~73.3% versus 52.5%). When fetal fibroblasts were cultured in PZM-3 and used as donor cells for NT embryos, the fusion rate of less than 10 passage was higher than those of 11 ~15 passage (604~75.
- In conclusion, the present study indicates that EGF and glucose have beneficial effect on the in vitro maturation of oocytes and p-ME have improved developmental ability of NT embryos. Furthermore, the developmental rate was improved when reconstruction was made with less than 10 passages subcultured fibroblast cells cultured in PZM-3 at 5% O2 gas atmospheres.
- In this study, the developmental rate to the blastocyst stage was 10.9~12.5% in PZM-3 and 9.8-11.1% in NCSU-23, respectively. 1m et al.
후속연구
- , 1996). Therefore, further research on the role of amino acids in the medium is needed.
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