이상 랩온어칩에서 사용된 생물분리 방법과 그 예를 소개하였다. 랩온어칩에서는 수백 마이크로미터 이하의 미세 채널을 사용하므로 유사한 크기의 채널을 사용하는 capillary electrophoresis에서 사용되었던 기법들이 가장 많이 활용되어왔으며, 랩온어칩 내에서 물질분리를 위한 기본 방법으로 적용되어왔다. 현재까지 CE에 사용되었던 기법들은 모두 랩온어칩 상에 구현된 바 있으며, 이러한 기술들은 랩온어칩의 활용 가능성 및 활용 분야 증대에 크게 기여하였다. 이외에도, laminar flow의 특성을 이용하거나, 막을 제작하거나, 추출 기법을 활용하는 등의 다양한 시도가 있었다. 그러나, high-throughput, 이동형 장비를 지향하는 랩온어칩에서 고전압을 사용하는 경우 활용에 제약을 가져올 수 있어, 용도에 맞는 적절한 분리기술의 개발 및 선택이 랩온어칩의 활용 가능성을 결정짓는 중요한 요인이 될 것으로 판단된다.
이상 랩온어칩에서 사용된 생물분리 방법과 그 예를 소개하였다. 랩온어칩에서는 수백 마이크로미터 이하의 미세 채널을 사용하므로 유사한 크기의 채널을 사용하는 capillary electrophoresis에서 사용되었던 기법들이 가장 많이 활용되어왔으며, 랩온어칩 내에서 물질분리를 위한 기본 방법으로 적용되어왔다. 현재까지 CE에 사용되었던 기법들은 모두 랩온어칩 상에 구현된 바 있으며, 이러한 기술들은 랩온어칩의 활용 가능성 및 활용 분야 증대에 크게 기여하였다. 이외에도, laminar flow의 특성을 이용하거나, 막을 제작하거나, 추출 기법을 활용하는 등의 다양한 시도가 있었다. 그러나, high-throughput, 이동형 장비를 지향하는 랩온어칩에서 고전압을 사용하는 경우 활용에 제약을 가져올 수 있어, 용도에 맞는 적절한 분리기술의 개발 및 선택이 랩온어칩의 활용 가능성을 결정짓는 중요한 요인이 될 것으로 판단된다.
Lab-on-a-chip is a miniaturized analytical device in which all of the procedures for the analysis of molecules are carried out, such as pretreatment, reaction, separation, detection, etc. Lab-on-a-chip has increasing concern as a device not only for rapid detection of molecules but also for high thr...
Lab-on-a-chip is a miniaturized analytical device in which all of the procedures for the analysis of molecules are carried out, such as pretreatment, reaction, separation, detection, etc. Lab-on-a-chip has increasing concern as a device not only for rapid detection of molecules but also for high throughput screening and point of care, because conventional laborious and time consuming analytical procedures can be substituted. Thus, a lot of microfabrication and analytical techniques for lab-on-a-chip have been developed with microstructures smaller than a few hundreds of micrometers. Separation of the molecules is one of the most important components of lab-on-a-chip, because effective separation method can simplify the design and can provide better sensitivity. The electrokinetic separation based on capillary electrophoresis is most widely employed technique in lab-on-a-chip for the control of fluids and the separation of molecules. In this article, bioseparation techniques and its applications realized in lab-on-a-chip are reviewed.
Lab-on-a-chip is a miniaturized analytical device in which all of the procedures for the analysis of molecules are carried out, such as pretreatment, reaction, separation, detection, etc. Lab-on-a-chip has increasing concern as a device not only for rapid detection of molecules but also for high throughput screening and point of care, because conventional laborious and time consuming analytical procedures can be substituted. Thus, a lot of microfabrication and analytical techniques for lab-on-a-chip have been developed with microstructures smaller than a few hundreds of micrometers. Separation of the molecules is one of the most important components of lab-on-a-chip, because effective separation method can simplify the design and can provide better sensitivity. The electrokinetic separation based on capillary electrophoresis is most widely employed technique in lab-on-a-chip for the control of fluids and the separation of molecules. In this article, bioseparation techniques and its applications realized in lab-on-a-chip are reviewed.
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문제 정의
1990년대 초 Manz, Harrison, Ramsey 등이 화학 분석 시 스템을 소형화한 microchip을 처음 제시한 이래(1, 2) 랩온어칩 (lab-on-a-chip)에 대한 관심이 급증하였다. Micro-total analysis system (p-TAS) 또는 microfluidic device로도 명명되 는 랩온어칩은 시료의 전처리, 반응, 분리, 분석, 결과분석 등 특정 물질의 분석에 필요한 일련의 모든 과정을 하나 의 기판 위에 소형화시킴으로써, 필요한 장소에서 필요한 시기에 대상 물질의 분석을 즉각적으로 수행할 수 있도록 하는 것이 목적이다. 이를 위하여 실리콘이나 폴리머 재질 의 기판 위에 수백 micrometer 이하의 미세 채널을 제작하고 이용함으로써 기존의 분석방법에 비하여 다양한 장점을 가진다.
이외에도 chromatography, 추출, filtration 등 물질의 분리에 필요한 다양한 기법들이 이미 랩온어칩상에서 구현되었다. 본 고 에서는 랩온어칩에 적용된 다양한 분리기술들 중 가장 많이 사용된 고전압을 이용한 분리 기법들의 간단한 원리와 랩온어칩에 적용한 예, 그리고 이외 랩온어칩에서 구현된 분리기법들을 정리하였다.
제안 방법
하나의 electrolyte# 이용하는 continuous system은 background electrolyte의 조성이 일정한 것과 모세관의 길이에 따라 변하는 것에 의하여 각각 kinetic process 와 steady-state process로 분류된다. 또한 시료가 두 개의 서로 다른 electrolyte 사이에서 이동하는 경우 discontinuous system으로 분류된다, 이하 각 방법에 대한 간단한 원리와 랩온어칩에 활용된 사례를 소개하였다.
후속연구
이외에도, laminar flow의 특성을 이용하거나, 막을 제작하거나, 추출기법을 활용하는 등의 다양한 시도가 있었다. 그러나, high-throughput, 이동형 장비를 지향하는 랩온어칩에서 고전압을 사용하는 경우 활용에 제 약을 가져올 수 있어, 용도에 맞는 적절한 분리기술의 개발 및 선택이 랩온어칩의 활용 가능성을 결정짓는 중요한 요인이 될 것으로 판단된다.
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