선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- 2 at 4℃) for 2 h, following two times washing with distilled water. Samples were serially dehydrated in ethanol (30, 50, 70, 80, 90, 100, 100, 100%), treated with isoamyl acetate for 15 min two times, critical point-dried in liquid CO2 using the Balzers CPD 010 (Balzers Instruments, Liechtenstein), mounted on aluminum stubs, and sputter-coated with gold and palladium using the Polaron SEM Coating Unit E5100 (Thermo VG Scientific, Beverly, MA, USA).
- The PCR products were cloned using a pGEM-T cloning kit (Promega, Madison, WI, USA) according to the manufacture’s instruction. The cloned fragment was directly sequenced using a Taq DyeDeoxy terminator Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and analyzed with an ABI PRISM 3700 DNA sequencer (Applied Biosystems, Foster City, CA, USA). The internal sequences of the PCR products were determined using four internal primers: p510r 5'-TAT TAC CGC GGC TGC TG-3', p364f 5'-GGC AGC AGT GGG GAA TAT TG3', p783f 5'-TAG ATA CCC TGG TAG TCC AC-3', and p1037f 5'-TCG TCA GCT CGT GTC GTG AG-3'.
- parasitica isolated in Korea showed a wide genetic variation that could reduce the effectiveness of biological control. Therefore, in the study attempts were made to isolate bacteria showing antifungal activity for biological control against C. parasitica.
이론/모형
- Phylogenetic position of strain Ta24 within the genus Streptomyces. The branching pattern was generated by the neighbor-joining method. The value at the inter nodes indicates the level of bootstrao support with 1000 replication.
- The internal sequences of the PCR products were determined using four internal primers: p510r 5'-TAT TAC CGC GGC TGC TG-3', p364f 5'-GGC AGC AGT GGG GAA TAT TG3', p783f 5'-TAG ATA CCC TGG TAG TCC AC-3', and p1037f 5'-TCG TCA GCT CGT GTC GTG AG-3'. The determined sequences were analyzed by the homology search of the BLAST program at National Center for Biotechnology Information and aligned with ClustalW software.
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