This study was performed to offer effective basic data for selection and improvement of Korean dairy cattle through identifying distributional properties among candidate genes (bovine butyrophilin, signal transducers and activators of transcription 5a, and prolactin hormone). In this study, polymorp...
This study was performed to offer effective basic data for selection and improvement of Korean dairy cattle through identifying distributional properties among candidate genes (bovine butyrophilin, signal transducers and activators of transcription 5a, and prolactin hormone). In this study, polymorphisms of candidate genes were identified and the relationships between loci and production traits of each gene were analyzed using frozen semen of Holstein bulls (19 proven and 77 candidates). In butyrophilin (BTN) locus, polymorphisms information contents (PIC) value of BTN2 (0.372) was higher than those of others (BTN1; 0.155, BTN3; 0.254, BTN4; 0.169). As a result of analysis of genotyping STAT5a, using single stranded conformational polymorphism (SSCP) method and microsatellite locus, PIC values were 0.189 and 0.457, respectively. And PIC value of prolactin hormone gene was 0.176. In the relationships between genotypes and production traits, BTN3 was associated with 305-day production traits (p<0.05). PTAs for B allele were such as 110.43, 88.28 and 75.25 in BTN1, 3, 4 and these values were higher than those of A allele, but in the case of BTN2, A allele with 154.19 was higher than that of B allele. The results obtained from using candidate genes may be used as an useful index for the genetic improvement of dairy cattle population in Korea, and further studies are needed.
This study was performed to offer effective basic data for selection and improvement of Korean dairy cattle through identifying distributional properties among candidate genes (bovine butyrophilin, signal transducers and activators of transcription 5a, and prolactin hormone). In this study, polymorphisms of candidate genes were identified and the relationships between loci and production traits of each gene were analyzed using frozen semen of Holstein bulls (19 proven and 77 candidates). In butyrophilin (BTN) locus, polymorphisms information contents (PIC) value of BTN2 (0.372) was higher than those of others (BTN1; 0.155, BTN3; 0.254, BTN4; 0.169). As a result of analysis of genotyping STAT5a, using single stranded conformational polymorphism (SSCP) method and microsatellite locus, PIC values were 0.189 and 0.457, respectively. And PIC value of prolactin hormone gene was 0.176. In the relationships between genotypes and production traits, BTN3 was associated with 305-day production traits (p<0.05). PTAs for B allele were such as 110.43, 88.28 and 75.25 in BTN1, 3, 4 and these values were higher than those of A allele, but in the case of BTN2, A allele with 154.19 was higher than that of B allele. The results obtained from using candidate genes may be used as an useful index for the genetic improvement of dairy cattle population in Korea, and further studies are needed.
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문제 정의
This study was performed to offer effective basic data for selection and improvement of Korean dairy cattle through analyzing genotypes and identifying distributional properties among candidate genes. And this study was also carried out to examine the possibility for discovering QTLs with candidate genes and for development of marker assist selection.
This study was performed to offer effective basic data for selection and improvement of Korean dairy cattle through analyzing genotypes and identifying distributional properties among candidate genes. And this study was also carried out to examine the possibility for discovering QTLs with candidate genes and for development of marker assist selection.
제안 방법
In this study, we performed two steps, firstly, we designed oligonucleotide primer pairs of BTN1, 2, 3, 4 and STAT5a SSCP, STAT5a microsatellite locus, and also prolactin gene with a view to detect the polymorphic region. And we examined the relationship between polymorphic regions with production traits data in Korean proven and young bulls.
대상 데이터
This study was performed using 19 Holstein proven and 77 candidate bulls’ (total 96 Holstein bulls) frozen semen from Dairy Cattle Improvement Center, National Agricultural Cooperation Federation.
This study was performed using 19 Holstein proven and 77 candidate bulls’ (total 96 Holstein bulls) frozen semen from Dairy Cattle Improvement Center, National Agricultural Cooperation Federation.
이론/모형
Polymorphisms Information Content (PIC) : Expected PIC value of each locus was calculated by using the method of Botstein et al. (1980).
Single stranded conformational polymorphism (SSCP) : Electrophoresis was performed for 5 h at 13 W in denatured polyacrylamide gel. The DNA bands were visualized by silver staining method.
성능/효과
PTA values of production traits (milk, fat, protein and SNF yield) of B allele in BTN1, 3 and 4 were higher than those of A allele however, in the case of BTN2, A allele was higher than B allele. PTA values of B allele and 114 bp allele in STAT5a gene were higher, and prolactin gene was not associated with 305-day production traits and PTA values of production traits (Tables 6 and 7).
Predicted transmitting ability (PTA) : After obtaining the predicted transmitting abilities, genotypes effects of B allele being such as 110.43, 88.28 and 75.25 in BTN1, 3, and 4 were higher than those of A allele, but in the case of BTN2, A allele with 154.19 was higher than this ofB allele. And 114 bp allele (12 repeated TG sequences) in STAT5a microsatellite was higher than others (Table 7).
Predicted transmitting ability (PTA) : After obtaining the predicted transmitting abilities, genotypes effects of B allele being such as 110.43, 88.28 and 75.25 in BTN1, 3, and 4 were higher than those of A allele, but in the case of BTN2, A allele with 154.19 was higher than this ofB allele. And 114 bp allele (12 repeated TG sequences) in STAT5a microsatellite was higher than others (Table 7).
후속연구
In this satudy, butyrophilin gene could be an important marker for dairy cattle breeding, considering the fact that the gene was not only associated with production traits, but also with disease traits. Then further studies about functions ofBTN and STAT5a gene in dairy cattle must be performed, and then BTN and STAT5a gene will be useful genetic marker for MAS. Therefore, the wide and various researches should be performed in candidate genes such as butyrophilin and STAT5a for the genetic improvement of dairy cattle, and be proposed as an useful index for selection improvement.
In this satudy, butyrophilin gene could be an important marker for dairy cattle breeding, considering the fact that the gene was not only associated with production traits, but also with disease traits. Then further studies about functions ofBTN and STAT5a gene in dairy cattle must be performed, and then BTN and STAT5a gene will be useful genetic marker for MAS. Therefore, the wide and various researches should be performed in candidate genes such as butyrophilin and STAT5a for the genetic improvement of dairy cattle, and be proposed as an useful index for selection improvement.
참고문헌 (19)
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