Yoon, Soon-Seek
(National Veterinary Research and Quarantine Service)
,
Park, J.W.
(National Veterinary Research and Quarantine Service)
,
Jean, Y.H.
(National Veterinary Research and Quarantine Service)
,
Kim, H.J.
(National Veterinary Research and Quarantine Service)
,
Han, B.
(College of Veterinary Medicine, Seoul National University)
,
Han, H.R.
(College of Veterinary Medicine, Seoul National University)
The integral relationship between the occurrence of lymphocyte nuclear pockets (LNPs) and BLV-infection was examined in Holstein-Friesian dairy cattle in Korea. Transmission electron microscope (TEM) was used to detect LNP in peripheral blood lymphocytes. Morphologically, the membranes of LNP were c...
The integral relationship between the occurrence of lymphocyte nuclear pockets (LNPs) and BLV-infection was examined in Holstein-Friesian dairy cattle in Korea. Transmission electron microscope (TEM) was used to detect LNP in peripheral blood lymphocytes. Morphologically, the membranes of LNP were composed of two layers of double nuclear membrane. The full thickness of LNP membranes including inner and outer nuclear membrane was 60 to 70 nm. LNP prevalence was different according to the bovine leukemia virus (BLV) infection status; in BLV-seropositive cattle, LNP prevalence was 48.4% and in BLV-seronegative cattle prevalence was 5.9%. Moreover, even in seropositive animals, leukemic group was the highest at 70% positive among the groups, followed by suspect group (42.4%) and aleukemic group (23.1%). Consequently, the numbers of LNP were increased in proportion to increase of the numbers of leukocytes among BLV-seropositive cattle. The numbers of LNP per lymphocyte were increased in BLVseropositive cattle compared with seronegative cattle. The mean numbers of LNP per 100-lymphocytes were 0.35, 0.77, 1.64 and 4.7 in BLV-seronegative, BLV-seropositive aleukemic, suspect and leukemic groups, respectively. Thus, it is reasonable that LNP test can be used as the one of the diagnostic criteria of BLV infection.
The integral relationship between the occurrence of lymphocyte nuclear pockets (LNPs) and BLV-infection was examined in Holstein-Friesian dairy cattle in Korea. Transmission electron microscope (TEM) was used to detect LNP in peripheral blood lymphocytes. Morphologically, the membranes of LNP were composed of two layers of double nuclear membrane. The full thickness of LNP membranes including inner and outer nuclear membrane was 60 to 70 nm. LNP prevalence was different according to the bovine leukemia virus (BLV) infection status; in BLV-seropositive cattle, LNP prevalence was 48.4% and in BLV-seronegative cattle prevalence was 5.9%. Moreover, even in seropositive animals, leukemic group was the highest at 70% positive among the groups, followed by suspect group (42.4%) and aleukemic group (23.1%). Consequently, the numbers of LNP were increased in proportion to increase of the numbers of leukocytes among BLV-seropositive cattle. The numbers of LNP per lymphocyte were increased in BLVseropositive cattle compared with seronegative cattle. The mean numbers of LNP per 100-lymphocytes were 0.35, 0.77, 1.64 and 4.7 in BLV-seronegative, BLV-seropositive aleukemic, suspect and leukemic groups, respectively. Thus, it is reasonable that LNP test can be used as the one of the diagnostic criteria of BLV infection.
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대상 데이터
Ninety-eight Holstein-Friesian dairy cattle were used in this study; BLV-noninfected (34 heads) and naturally infected (64 heads). They reared in Chungnam-Do, Chungbuk-Do and Kyeonggi-Do of Korea.
Ninety-eight Holstein-Friesian dairy cattle were used in this study; BLV-noninfected (34 heads) and naturally infected (64 heads). They reared in Chungnam-Do, Chungbuk-Do and Kyeonggi-Do of Korea. All examined cattle were female and overthree years old.
성능/효과
As the result ofLNP test, there was a difference on LNP expression between BLV-seropositive and -seronegative Holstein-Friesian dairy cattle, and also among the seropositive groups, the proportion of LNP-positive cattle was increased in proportion with the increase of the leukocyte number in peripheral blood. Therefore, method of LNP detection may be used as a compensatory test to PCR for the early diagnosis of the BLV infection.
As the result ofLNP test, there was a difference on LNP expression between BLV-seropositive and -seronegative Holstein-Friesian dairy cattle, and also among the seropositive groups, the proportion of LNP-positive cattle was increased in proportion with the increase of the leukocyte number in peripheral blood. Therefore, method of LNP detection may be used as a compensatory test to PCR for the early diagnosis of the BLV infection.
There was a prominent difference in LNP expression between BLV-seropositive and -seronegative group (Table 1), and it also showed that the number of lymphocytes with LNP increased particularly in proportion to the increase of the number of leukocyte in peripheral blood (Table 2). In the group 1, the rate of LNP suspect cattle was high, while the rate of LNP positive cattle was low (Table 1).
There was a prominent difference in LNP expression between BLV-seropositive and -seronegative group (Table 1), and it also showed that the number of lymphocytes with LNP increased particularly in proportion to the increase of the number of leukocyte in peripheral blood (Table 2). In the group 1, the rate of LNP suspect cattle was high, while the rate of LNP positive cattle was low (Table 1).
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