In order to understand the molecular basis of chicken heterosis in reproduction traits, mRNA differential display (DDRT-PCR) methods were used to analyze the differential gene expression of ovary tissue between hybrids and their parental lines in a 4${\times}$4 diallel cross, involving 4 ...
In order to understand the molecular basis of chicken heterosis in reproduction traits, mRNA differential display (DDRT-PCR) methods were used to analyze the differential gene expression of ovary tissue between hybrids and their parental lines in a 4${\times}$4 diallel cross, involving 4 chicken breeds, which were White Plymouth Rock (E), CAU Brown (D), Silkies (C) and White Leghorn (A). Total of 331 differential displayed cDNA bands from 1,161 were displayed in the 4${\times}$4 diallel cross combinations with 30 pairs of primers, which shows the differences of gene expression between hybrids and their parental lines were very obvious in quantity and quality. Seven types of differential expression patterns were found: Co-dominance expressed pattern (T1), under-expression of parental fragments in hybrids (T2), over-expression of parental fragments in hybrids (T3), hybrid-absence expressed pattern (T4), single parentspecific expressed pattern (T5), dominant expression fragments of single parent in hybrids (T6), hybrid-specific expressed pattern (T7). Correlation analysis indicated that there were significant correlations between the pattern of T3 and the heterosis percentage of egg number of 32-week and 42-week old chickens(p<0.01), while there were negative significant correlations between the pattern of T7 and the heterosis percentage of egg number of 32-week and 42 week-old birds (p<0.01).
In order to understand the molecular basis of chicken heterosis in reproduction traits, mRNA differential display (DDRT-PCR) methods were used to analyze the differential gene expression of ovary tissue between hybrids and their parental lines in a 4${\times}$4 diallel cross, involving 4 chicken breeds, which were White Plymouth Rock (E), CAU Brown (D), Silkies (C) and White Leghorn (A). Total of 331 differential displayed cDNA bands from 1,161 were displayed in the 4${\times}$4 diallel cross combinations with 30 pairs of primers, which shows the differences of gene expression between hybrids and their parental lines were very obvious in quantity and quality. Seven types of differential expression patterns were found: Co-dominance expressed pattern (T1), under-expression of parental fragments in hybrids (T2), over-expression of parental fragments in hybrids (T3), hybrid-absence expressed pattern (T4), single parentspecific expressed pattern (T5), dominant expression fragments of single parent in hybrids (T6), hybrid-specific expressed pattern (T7). Correlation analysis indicated that there were significant correlations between the pattern of T3 and the heterosis percentage of egg number of 32-week and 42-week old chickens(p<0.01), while there were negative significant correlations between the pattern of T7 and the heterosis percentage of egg number of 32-week and 42 week-old birds (p<0.01).
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제안 방법
4x4 diallel cross involving 4 chicken breeds, which were White Plymouth Rock (EE), CAU-Brown (DD), Silkies (CC) and White Leghorn (AA), and the 12 hybrids, which were ED, DE, DC, CD, DA, AD, AC, CA, AE, EA, CE and EC were used in this study. CAU-Brown is a Brown-egg breed which was high yield and cultivated by researchers in China Agricultural University in 1991.
In the study reported here, differential gene expression patterns in 32 week-old ovary of 12 hybrids relative to their parental lines were compared by means of DDRT-PCR. In addition the correlation analyses of various differential gene expression patterns with the performance and heterosis of reproduction traits were evaluated in order to provide more documentation on the molecular mechanism of chicken reproduction traits heterosis.
성능/효과
The percentage of various patterns of differentially displayed cDNA fragments is as following. Co-dominance expressed pattern (T1 = 12.69%), Under-expression of parental fragments in hybrids (T2 = 13.29%), Over-expression of parental fragments in hybrids (T3 = 13.29%), Hybrid- absence expressed pattern (T4 = 6.34%), Single parent-specific expressed pattern (T5 = 22.66%), Dominant expression fragments of single parent in hybrids (T6 = 18.73%), Hybrid-specific expressed pattern (T7 = 12.99%). In them, the quantitative difference include T1, T2 and T3; as the qualitative difference include T4, T5, T6 and T7 (Table 2), electrophoresis profiles of seven kinds of patterns were in Figure 2.
Correlation analysis showed that: there is significant correlation between the pattern of T3 and the heterosis percentage of egg number of 32-week and 42-week old (p<0.01), while there are negative significant correlation between the pattern of T7 and the heterosis percentage of egg number of 32-week and 42 week-old (p<0.01).
Correlation analysis showed that: there is significant correlation between the pattern of T3 and the heterosis percentage of egg number of 32-week and 42-week old (p<0.01), while there are negative significant correlation between the pattern of T7 and the heterosis percentage of egg number of 32-week and 42 week-old (p<0.01).
From the 30 primer combinations, a total of 1,631 bands were displayed, and 71.18% (1,161 out of 1,631) can be repeated in duplicate DDRT-PCR amplification, in which 331 cDNA bands (28.51%) were found to be polymorphic between 12 hybrids and their parents. For each of the 30 primer combinations, an average of 11.
From the 30 primer combinations, a total of 1,631 bands were displayed, and 71.18% (1,161 out of 1,631) can be repeated in duplicate DDRT-PCR amplification, in which 331 cDNA bands (28.51%) were found to be polymorphic between 12 hybrids and their parents. For each of the 30 primer combinations, an average of 11.
Results in the study revealed that hybrid F1 specific expression genes appeared to restrain the heterosis forming of egg number traits; while Over-expression of parental genes in hybrid F1 appeared to reinforce the heterosis forming of egg number traits.
후속연구
In the study reported here, differential gene expression patterns in 32 week-old ovary of 12 hybrids relative to their parental lines were compared by means of DDRT-PCR. In addition the correlation analyses of various differential gene expression patterns with the performance and heterosis of reproduction traits were evaluated in order to provide more documentation on the molecular mechanism of chicken reproduction traits heterosis.
In the study reported here, differential gene expression patterns in 32 week-old ovary of 12 hybrids relative to their parental lines were compared by means of DDRT-PCR. In addition the correlation analyses of various differential gene expression patterns with the performance and heterosis of reproduction traits were evaluated in order to provide more documentation on the molecular mechanism of chicken reproduction traits heterosis.
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