[논문]Effect of Supplementary Dietary L-carnitine and Yeast Chromium on Lipid Metabolism of Laying Hens

Du, Rong; Qin, Jian; Wang, Jundong; Pang, Quanhai; Zhang, Chunshan; Jiang, Junfang

doi:10.5713/ajas.2005.235

Effect of Supplementary Dietary L-carnitine and Yeast Chromium on Lipid Metabolism of Laying Hens 원문보기

Asian-Australasian journal of animal sciences, v.18 no.2, 2005년, pp.235 - 240

Du, Rong (Working at research for Doctor Degree in China Agricultural University) , Qin, Jian (College of Animal Science and Veterinary Medicine, Shanxi Agricultural University) , Wang, Jundong (College of Animal Science and Veterinary Medicine, Shanxi Agricultural University) , Pang, Quanhai (College of Animal Science and Veterinary Medicine, Shanxi Agricultural University) , Zhang, Chunshan (College of Animal Science and Veterinary Medicine, Shanxi Agricultural University) , Jiang, Junfang (College of Animal Science and Veterinary Medicine, Shanxi Agricultural University)

Abstract ▼ AI-Helper

Two hundred and eighty-eight 21-week-old Hyline Brown laying hens were randomly allotted to 9 treatments, 32 birds for each treatment. A 3${\times}$3 (chromium${\times}$L-carnitine) factorial experiment was designed to investigate the single and interactive effects of adding yeast chromium (0, 400 and 600 ${\mu}g/kg$) and L-carnitine (0, 50 and 100 mg/kg) to corn-soybean diets on lipid metabolism of laying hens for 7 weeks. The results showed that 600 ${\mu}g/kg$ chromium or 100 mg/kg L-carnitine had significant effects on most indices of lipid metabolism (p<0.05 or 0.01). There were significant interactions on the concentration of liver triglycerides, egg yolk cholesterol, abdominal fat percentage between chromium and L-carnitine (p=0.0003-0.0500). Adding 400 ${\mu}g/kg$ chromium and 100 mg/kg Lcarnitine simultaneously was the best for reducing egg yolk cholesterol and adding 400 ${\mu}g/kg$ chromium and 50 mg/kg L-carnitine at the same time was the best for reducing abdominal fat percentage. There was no side effect on production performance of laying hens while chromium or (and) L-carnitine reduced liver lipid, abdominal fat and egg yolk cholesterol.

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제안 방법

, 2004) showed that there were significant interactions on the lipid metabolism of broiler chickens between Cr and L-carnitine. The stage (transition to highproduction) in which the lipid metabolism changes clearly was selected, investigate the and the experiment was conducted to single and interactive effects of Cr and Lcarnitine on lipid metabolism of laying hens.
The ways of determination of total cholesterol (TC), triaclyglycerol (TG), High (Low) density lipoprotein cholesterol (HDL-C, LDL-C), Apolipoprotein AI or B (APOAI and APOB), Glucose (GLu) were respectively CHOD-PAP, GPO-PAP, PAT-Mg2+ (PVS), transmission turbidimetry and GOD-PAP.
The ways of determination of total cholesterol (TC), triaclyglycerol (TG), High (Low) density lipoprotein cholesterol (HDL-C, LDL-C), Apolipoprotein AI or B (APOAI and APOB), Glucose (GLu) were respectively CHOD-PAP, GPO-PAP, PAT-Mg2- (PVS), transmission turbidimetry and GOD-PAP.

대상 데이터

Two hundreds and eighty-eight 21-week-old Hyline Brown laying hens were randomly allocated to 9 treatments, 32 birds for each treatment (4 replicates). A 3x3 (Crxcarnitine) factorial experiment was designed, in which Cr (0, 400 and 600 µg/kg) and L-carnitine (0, 50 and 100 mg/kg) were supplemented.

데이터처리

Data were analyzed by F value using two factors, 3x3 levels procedures of SAS statistical software, and then Duncan’s Multiple Range Test for Variable was carried out, with the standard probabilities of 0.01 and 0.05.
Data were analyzed by F value using two factors, 3x3 levels procedures of SAS statistical software, and then Duncan’s Multiple Range Test for Variable was carried out, with the standard probabilities of 0.01 and 0.05.

성능/효과

At the end of experiment, adding Cr or (and) L-carnitine did not affect production performance of laying hens (p>0.05), but mean egg weight and egg mass tended to increase although daily percent lay tended to reduce (Table 2)
Compared with the control group, the level of 400, 600 μg/kg Cr resulted in decrease ofliver TG by 16.55% and 13.28% (p<0.05), decrease ofliver TC by 17.65% and 12.16% (p<0.05), decrease ofyolk TC by 12.67% and 12.11% (p<0.05), respectively.
Compared with the control group, the level of 400, 600 μg/kg Cr resulted in increase of serum FFA by 23.35% (p<0.01) and 9.11% (p<0.05), increase of serum HDL-C by 51.81% and 22.89% (p<0.05), increase of serum APOAI by 47.11% (p<0.01) and 19.94% (p<0.05); decrease of serum APOB by 29.01% and 45.43% (p<0.01), respectively.
Adding chromium or L-carnitine singly (n=8) : Adding Cr or L-carnitine alone had significant effects on the contents of liver TG, liver TC, yolk TC and abdominal fat weight or percentage (AFW or AFP). Compared with the control group, the level of 400, 600 μg/kg Cr resulted in decrease ofliver TG by 16.55% and 13.28% (p<0.05), decrease ofliver TC by 17.65% and 12.16% (p<0.05), decrease ofyolk TC by 12.67% and 12.11% (p<0.05), respectively. Only 600 μg/kg Cr significantly reduced AFP by 28.
Adding chromium or L-carnitine single (n=8) : Adding Cr alone had significant effects on the contents of serum FFA, HDL-C, APOAI and APOB. Compared with the control group, the level of 400, 600 μg/kg Cr resulted in increase of serum FFA by 23.35% (p<0.01) and 9.11% (p<0.05), increase of serum HDL-C by 51.81% and 22.89% (p<0.05), increase of serum APOAI by 47.11% (p<0.01) and 19.94% (p<0.05); decrease of serum APOB by 29.01% and 45.43% (p<0.01), respectively. Only 600 μg/kg Cr significantly reduced serum TG and LDL-C, respectively by 31.
Compared with the control group, the level of 50 and 100 mg/kg L-carnitine increased serum FFA by 10.85% and 9.85% (p<0.05), increased serum HDL-C by 45.78% (p<0.05) and 110.8% (p<0.01), decreased serum APOB by 21.58% and 15.73% (p<0.05).
Compared with the control group, the level of 50 and 100 ㎎/㎏ L-carnitine increased serum FFA by 10.85% and 9.85% (p<0.05), increased serum HDL-C by 45.78% (p<0.05) and 110.8% (p<0.01), decreased serum APOB by 21.58% and 15.73% (p<0.05).
Our results showed that 600 μg/kg Cr had significant effects on abdominal fat, liver TG, serum TG, and serum FFA of laying hens at the end of experiment.
Our results showed that 600 μg/kg Cr had significant effects on abdominal fat, liver TG, serum TG, and serum FFA of laying hens at the end of experiment. The mechanism may be as follows: Cr, as a component of glucose tolerance factor (GTF), enhances the function of insulin, increasing intake of serum TG into tissue, promoting the transformation of energy, supplying more ATP to tissue, inhibiting decomposition of cAMP (Bian, 1997), promoting the phosphorylation of hormone sensitive lipase (HSL) (Ecan, 1992) and reducing the combination of insulin with adipocytes (Liu, 1990).
Adding 100 mg/kg L-carnitine reduced abdominal fat, liver TG, serum TG, APOB and LDL-C concentrations and increased serum FFA concentrations in our experiment. The results showed that L-carnitine promoted decomposition of triglyceride in fatty tissue and inhibited synthesis of liver triglyceride by the feedback control of ^-oxidation (offatty acid) increasing.
Adding 100 mg/kg L-carnitine reduced abdominal fat, liver TG, serum TG, APOB and LDL-C concentrations and increased serum FFA concentrations in our experiment. The results showed that L-carnitine promoted decomposition of triglyceride in fatty tissue and inhibited synthesis of liver triglyceride by the feedback control of ^-oxidation (offatty acid) increasing.
Yolk TC was not highly related to serum TC according to the results in our test: yolk TC reduced significantly with the supplemention of 600 μg/kg Cr, but serum TC had only declining tendency.
Yolk TC was not highly related to serum TC according to the results in our test: yolk TC reduced significantly with the supplemention of 600 μg/kg Cr, but serum TC had only declining tendency. The reason may be that the content of egg TC depends on lipoprotein concentration in yolk but not serum TC concentration (Griffin, 1992).

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