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Changes in Serum Protein Profile, Cholesterol and Blood Glucose during Endotoxic Shock in Buffalo Calves Supplemented with Vitamin E and Selenium 원문보기

Asian-Australasian journal of animal sciences, v.18 no.2, 2005년, pp.192 - 196  

Sharma, Neeraj (Department of Veterinary Biochemistry, College of Veterinary Science) ,  Singha, S.P.S. (Department of Veterinary Biochemistry, College of Veterinary Science) ,  Ahuja, C.S. (Department of Veterinary Biochemistry, College of Veterinary Science)

Abstract ▼ AI-Helper 아이콘AI-Helper

A study was conducted to monitor the changes in serum protein profile, cholesterol and blood glucose during endotoxic shock in buffalo calves and also to assess the role of prophylactic supplementation of vitamin E and selenium in alleviating the endotoxic effects. Fifteen male buffalo calves (6-8 m...

주제어

#Endotoxin   #Vit   #E and Se   #Serum Biochemical Changes   #Buffalo Calves  

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제안 방법

  • Fifteen male buffalo calves (aged 6-8 months) selected for this study were procured from the local market and were maintained as per standard management and feeding conditions practiced at the dairy farm, P. A. U. Ludhiana.
  • Therefore this study was planned to monitor the changes in serum protein profile, cholesterol and blood glucose before and after induction of endotoxic shock in buffalo cal.,es with/without prophylactic supplementation of vitamin E and selenium.
  • Damage to liver during endotoxic shock, thus, may results in alterations in the profiles of these metabolites in the blood. Therefore this study was planned to monitor the changes in serum protein profile, cholesterol and blood glucose before and after induction of endotoxic shock in buffalo calves with/without prophylactic supplementation of vitamin E and selenium.

이론/모형

  • , 1951). SDS-PAGE used for the separation of different protein fractions was done by the method of Laemmli (1970). For statistical analysis the methods detailed by Snedecor and Cochran (1976) were followed.
  • Blood glucose was estimated by the method of Folin and Wu (1920). Serum cholesterol and total proteins were analyzed by the modified method of Zak (1957) and biuret method of Reinhold (1953), respectively. Serum albumin was determined by chemistry analyzer (RA-50, Miles India Ltd.
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참고문헌 (25)

  1. Bhullar, P. 1998. Physiological studies on antioxidant status in different age groups of buffaloes. M. V. Sc. Thesis, Punjab Agricultural University, Ludhiana, India 

  2. Constable, P. D., K. Yvorchuck and G. S. Jeans. 1993. The clinical use of hypertonic solution in food animals with haemorrhagic and endotoxic shock. Agriculture Practice 14:6-11 

  3. Engel, F. L., M. G. Winton and C. N. H. Long. 1943. Biochemical studies on shock. The metabolism of amino acids and carbohydrates during haemorrhagic shock in rats. J. Expt Med. 77:397-410. 

  4. Folin, O. and H. Wu. 1920. A system of blood analysis: Supplement 1A simplified and improved method for determination of sugar. J. Biol. Chem. 41:367-374. 

  5. Gruys, E., M. J. Obwolon and J. M. Mathilda. 1994. Diagnostic significance of major acute phase proteins in veterinary clinical chemistry. Vet. Bull. 64:1009-1018. 

  6. Heinrich, P. C., J. V. Castell and T. Andus. 1990. Interleukin-6 and the acute phase response. Biochem. J. 265:621-636. 

  7. Kaneko, J. J. 1997. Clinical Biochemistry of Domestic Animals. 5th Edn. pp. 120-138. Academic Press, New York, USA. 

  8. Kumar, R. 1989. Hormonal and biochemical profile during developmental and reproductive stages in buffalo calves. M. V. Sc. thesis, Punjab Agricultural University, Ludhiana, India. 

  9. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277:680-685. 

  10. Lowry, O. H., N. J. Rosenbrough, A. L. Farr and R. J. Randall. 1951. Protein measurement with Folin-phenol reagent. J. Biol Chem. 193:265-275. 

  11. Mayes, P. A. 1996. Structure and functions of lipid soluble vitamins. In: Harper’s Biochemistry. 24th Edn. pp. 614-624. 

  12. Appleton and Lange, Stamford, Connecticut, USA. Naylor, J. M. and D. S. Kronfeld. 1985. In vivo studies on hypoglycemia and lactic acidosis in endotoxic shock. Am. J. Physiol. 248:309-316. 

  13. Paul, B. S. and V. P. Vadlamudi. 1974. Anote on some normal biochemical constituents of blood of Murrah buffalo calves. Ind. J. Anim. Sci. 44:273-275. 

  14. Philips, R. W., L. G. Chandrasena, M. S. Hand, D. Giesecke, G. Dirksen and M. Stangassinger. 1981. Metabolic effects of endotoxin in calves and pigs. Proceedings IV International Conference in Farm Animals, pp. 111-115. Munchen, Germany. 

  15. Portoles, M. T., M. Catala, A. Auton and R. Pagani. 1996. Hepatic response to oxidative stress induced by E. coli endotoxin: glutathione as an index of acute phase during endotoxic shock. Mol. and Cell. Biochem. 159:115-121. 

  16. Reinhold, J. G. 1953. Total protein, albumin and globulin. In: Standard Methods of Clinical Chemistry. Vol. I, pp. 88. Academic Press, New York, USA. 

  17. Sakaguchi, S., N. Kanda, C. C. Hsu and O. Sakaguchi. 1981a. Lipid peroxide formation and membrane damage in endotoxin poisoned mice. Microbiology and Immunology 25 (3):229-244. 

  18. Sammalkorpi, K. T., V. V. Valtonen and L. P. J. Maury. 1990. Lipoproteins and acute phase response during acute infection. Interrelationships between C-reactive protein and serum amyloid-A protein and lipoproteins. Animals Medicine 22:397-401. 

  19. Sandhu, T. S. and S. P. S. Singha. 2003. Effect of prophylactic supplementation of vitamin E and Se on antioxidant enzymes during endotoxic shock in buffalo calves. Asian-Aust. J. Anim. Sci. 16(11):1577-1582. 

  20. Singh, D. V. 2000. Physiological and pharmacological studies in bovine endotoxic shock and its treatment. Ph. D. dissertation, Punjab Agricultural University, Ludhiana, India. 

  21. Singh, D. V. and S. P. S. Sodhi. 1992. Effect of shock on certain biochemical profiles of blood in calves. Ind. J. Anim. Sci. 62:1031-1036. 

  22. Singh, J. 1979. An investigation into pathophysiology and treatment of shock in bovines. Ph. D. dissertation, Punjab Agricultural University, Ludhiana, India. 

  23. Singh, R. 1998. Studies on antioxidant status of normal cycling, delayed pubertal and anestrous buffaloes. Ph. D. dissertation, Punjab Agricultural University, Ludhiana, India. 

  24. Snedecor, G. W. and W. G. Cochran. 1976. Statistical Methods. Iowa State University Press, Iowa, USA. 

  25. Zak, B. 1957. Simple and rapid microtechnique for serum total cholesterol. Am. J. Clin. Path. 27:583. 

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