In this comparative study, the effects of temperature, pH, and bactericides on the growth of Erwinia pyrifoliae and Erwinia amylovora were investigated. The maximum temperature for the growth of both Erwinia species was estimated to be $36{\circ}C$. The maximum specific growth rates of E....
In this comparative study, the effects of temperature, pH, and bactericides on the growth of Erwinia pyrifoliae and Erwinia amylovora were investigated. The maximum temperature for the growth of both Erwinia species was estimated to be $36{\circ}C$. The maximum specific growth rates of E. pyrifoliae and E. amylovora were observed at $27{\circ}C$ and $28{\circ}C$, respectively, and no significant growth differences were shown at their optimum temperatures. However, at lower temperatures ranging from 12-$21{\circ}C$, E. pyrifoliae showed higher growth rates with doubling times shorter than those of E. amylovora. Distinct growth rates at these temperatures revealed that E. pyrifoliae is more cold-tolerant than E. amylovora. The optimum pH for the growth of both pathogens was 7.5 and growth was not seen at pH ${\le}$ 5.0 and ${\ge}$ 10.0. These results showed that the effect of pH on the growth of E. pyrifoliae and E. amylovora was similar. Minimum inhibitory concentrations (MICs) of copper sulfate, oxolinic acid, streptomycin, and tetracycline, which inhibited growth of E. pyrifoliae and E. amylovora, were determined. The strains of both pathogens were able to grow at 0.08-0.32 mM copper sulfate, but not at higher concentrations. However, none of the tested strains grew in the presence of oxolinic acid (0.001 mM), streptomycin (0.1 mM), and tetracycline (0.01 mM) concentrations. These results suggested that all strains of both Erwinia species were sensitive to tested bactericides and indicated no occurrence of resistant strains of E. pyrifoliae in Korea.
In this comparative study, the effects of temperature, pH, and bactericides on the growth of Erwinia pyrifoliae and Erwinia amylovora were investigated. The maximum temperature for the growth of both Erwinia species was estimated to be $36{\circ}C$. The maximum specific growth rates of E. pyrifoliae and E. amylovora were observed at $27{\circ}C$ and $28{\circ}C$, respectively, and no significant growth differences were shown at their optimum temperatures. However, at lower temperatures ranging from 12-$21{\circ}C$, E. pyrifoliae showed higher growth rates with doubling times shorter than those of E. amylovora. Distinct growth rates at these temperatures revealed that E. pyrifoliae is more cold-tolerant than E. amylovora. The optimum pH for the growth of both pathogens was 7.5 and growth was not seen at pH ${\le}$ 5.0 and ${\ge}$ 10.0. These results showed that the effect of pH on the growth of E. pyrifoliae and E. amylovora was similar. Minimum inhibitory concentrations (MICs) of copper sulfate, oxolinic acid, streptomycin, and tetracycline, which inhibited growth of E. pyrifoliae and E. amylovora, were determined. The strains of both pathogens were able to grow at 0.08-0.32 mM copper sulfate, but not at higher concentrations. However, none of the tested strains grew in the presence of oxolinic acid (0.001 mM), streptomycin (0.1 mM), and tetracycline (0.01 mM) concentrations. These results suggested that all strains of both Erwinia species were sensitive to tested bactericides and indicated no occurrence of resistant strains of E. pyrifoliae in Korea.
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제안 방법
pyrifoliae. In this investigation, we evaluated the sensitivity of E. pyrifoliae to copper sulfate, oxolinic acid, streptomycin, and tetracycline for the selection of effective bactericides against this pathogen.
MICs were determined to be the concentrations at which tested bactericides inhibited in vitro growth of theses pathogens. The MGY agar was prepared, cooled to 50℃, and amended with filter-sterilized copper sulfate to final concentrations of 0.01, 0.02, 0.04, 0.08, 0.16, 0.32, 0.64, and 1.2 mM; oxolinic acid to 0.001, 0.002, 0.004, 0.008, and 0.016 mM; streptomycin to 0.1, 0.2, 0.4, 0.8, 1.60, and 3.20 mM; and tetracycline to 0.01, 0.02, 0.04, 0.08, 0.16, 0.32, and 0.64 mM. All strains were cultured overnight and O.
성능/효과
1). Based on these results, it was concluded that E. pyrifoliae is more c이d-tolerant than E. amylovora. Regarding the epidemic survey of shoot blight disease, E.
0. The most favorable pH was found at 7.5 for both pathogens, which showed optim니rm growth with 아doubling times of 1.47 ±0.03 h, 1.49 ± 0.08 h, and 1.44 ±0.07 h in E. pyrifoliae WT3, E. pyrifoliae Ep 16, and E. amylovora ATCC15580, respectively (Table 3, Fig. 2). These results showed similar effects of pH on the growth of these two pathogens, alth이jgh some variation was observed between acidic (pH 5.
참고문헌 (19)
Ark, P. A. 1937. Variability in the fire blight organism, Erwinia amylovora. Phytopathology 27: 1-28
Beer, S. V., Kim, J. H., Zumoff, C. H., Bogdanove, A. J., Laby, R. J., Gustafson, H. L., Momol, T., Aldwinckle, H. S., Tanii, A. and Tamura, O. 1996. Characterization of bacteria that cause 'bacterial shoot blight of pear' in Japan. Acta Hortic. 411: 179-181
Begot, C., Desnier, I., Daudin, J. D., Labadie, J. C. and Lebert, A. 1996. Recommendations for calculating growth parameters by optical density measurements. J. Microbiol. Methods 25:225-232
Billing, E., Baker, L. A. E., Crosse, J. E. and Garrett, C. M. E. 1961. Characteristics of English isolates of Erwinia amylovora. J. Appl. Bacteriol. 24: 195-211
Bonn, W G and Van der Zwet, T. 2000. Distribution and economic importance of fire blight. In Vanneste J. L. (ed), Fire blight: The disease and its causative agent, Erwinia amylovora. CABI publishing, UK, pp 37-53
Ingraham, J. L., Maloe, O. and Neidhardt, F. C. 1983. Growth of bacterial cell. Sinauer Associates Inc. Massachusetts, USA
Jock, S., Jacob, T., Kim, W-S., Hilgebrand, M., Vosberg, H. P. and Geider, K. 2003. Instability of short-sequence DNA repeats of pear pathogenic Erwinia strains from Japan and Erwinia amylovora fruit tree and raspberry strains. Mol. Genet. Genomics 268:739-749
Kim, W-S., Gardan, L., Rhim, S. L. and Geider, K. 1999. Erwinia pyrifoliae sp. nov., a noval pathogen affecting Asian pear trees (Pyrus pyrifoliae Nakai). Intl. J. Sys. Bacteriol. 49:899-906
Kim, W-S., Jock, S., Paulin, J. P., Rhim, S.-L. and Geider, K. 2001. Molecular detection and differentiation of Erwinia pyrifoliae and Host range analysis of the Asian pear pathogen. Plant Dis. 85:1183-1188
Maxson-Stein, K., McGhee, G. C., Smith, J. J., Jones, A. L. and Sudin, G. W. 2003. Genetic analysis of a pathogenic Erwinia sp. isolated from pear in Japan. Phytopathology 93: 1393-1399
Mc Manus, P. S., and Jones, A. L. 1994. Epidemiology and genetic analysis of streptomycin-resistant Erwinia amylovora from Michigan and evaluation of oxytetracycline for control. Phytopathology. 84:627-633
Mizuno, A., Sato, S., Kaeai, A. and Nishiyama, K. 2000. Taxonomic position of the causal pathogen of bacterial shoot blight of pear. J. Gen. Plant. Pathol. 66:48-58
Pirt, S. J. 1975. Principles of microbe and cell cultivation. Blackwell, Oxford, UK
Rhim, S.-L., Voelkisch, B., Gardan, L., Paulin, J. P., Langlotz, C., Kim, W-S. and Geider, K. 1999. An Erwinia species, different from E. amylovora cause a necrotic disease of Asian pear trees. Plant Pathol. 48:514-520
Shrestha, R., Koo, J. H., Park, D. H., Hwang, I., Hur, J. H. and Lim, C. K. 2003. Erwinia pyrifoliae, a causal endemic pathogen of shoot blight of Asian pear tree in Korea. Plant Pathol. J. 19:204-300
Shrestha, R., Tsuchiya, K., Baek, S. J., Bae, H. N., Hwang, I., Hur, J. H. and Lim, C. K. 2005. Identification of dspEF. hrpW, and hrpN loci and characterization of the $hrpN_{Ep}$ gene in Erwinia pyrifoliae. J. Gen. Plant Pathol. 71 (in press)
Young, J. M., Leuketina, R. C. and Marshal, A. M. 1977. The effects on temperature on growth in vitro of Pseudomonas syringae and Xanthomonas pruni. J. Appl. Bacteriol. 42:345-354
Van der Zwet, T. and Keil, H. L. 1979. Fire blight - A bacterial disease of rosaceous plants. United States Department of Agriculture Handbook 510, Washington DC, USA
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