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Identification of Recombinant Subtilisins

Journal of microbiology and biotechnology v.15 no.1 , 2005년, pp.35 - 39  
CHOI , NACK-SHICK (Proteome Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, Department of Microbiology, Chungnam National University ) ;  YOO, KI-HYUN ( Proteome Research Laboratory, Korea Research Institute of Bioscience and Biotechnology ) ;  YOON, KAB-SEOG ( Proteome Research Laboratory, Korea Research Institute of Bioscience and Biotechnology ) ;  CHANG, KYU-TAE ( Primate Research Laboratory, Korea Research Institute of Bioscience and Biotechnology ) ;  MAENG, PIL-JAE ( Department of Microbiology, Chungnam National University ) ;  KIM, SEUNG-HO ( Proteome Research Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Abstract

To identify the activity of recombinant subtilisins (subtilisin BPN' and subtilisin Carlsberg), three different zymography methods, SDS-fibrin zymography (SDS-FZ), reverse fibrin zymography (RFZ), and isoelectric focusingfibrin zymography (IEF-FZ), were used. The recombinant subtilisins BPN' and Carlsberg did not migrate into the electrophoretic field based on a Laemmli buffer system, instead forming a "binding mode" at the top part of the separating gels with the SDS-FZ and RFZ techniques. Yet, this problem was resolved when using IEF-FZ with a pH range from 3 to 10. In addition, all these methods enabled the activity of a recombinant pro-subtilisin DJ-4 to be detected without a refolding pathway.

주제어

#Bacillus   #binding mode   #isoelectric focusing   #fibrin zymography   #recombinant subtilisin  

저자의 다른 논문

Yoon, Kab-Seog(7) Chang, Kyu-Tae(11) Maeng, Pil Jae(26) Kim, Seung-Ho(16)

참고문헌 (28)

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이 논문을 인용한 문헌 (8)

  1. 2005. "" Journal of microbiology and biotechnology, 15(1): 72~79 
  2. 2006. "" Journal of microbiology and biotechnology, 16(3): 457~464 
  3. 2006. "" Journal of microbiology and biotechnology, 16(5): 804~807 
  4. 2007. "" Journal of microbiology and biotechnology, 17(9): 1469~1476 
  5. 2007. "" Journal of microbiology and biotechnology, 17(5): 761~768 
  6. 2008. "" Journal of microbiology and biotechnology, 18(6): 1076~1080 
  7. 2010. "" Journal of microbiology and biotechnology, 20(2): 370~374 
  8. 2010. "" Journal of the Korean Society for Applied Biological Chemistry, 53(1): 56~61 

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