• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보


Microbacterium laevaniformans levansucrase synthesized various hetero-oligosaccharides by transferring fructosyl residue from sucrose to various saccharides as acceptors. The acceptor specificity test showed that reducing saccharides were more favorable acceptors than nonreducing saccharides. The transfructosylated product, fructosyl galactose, was produced in the presence of D-galactose as an acceptor. The chemical structure of the resulting fructosyl galactose was analyzed by yeast invertase and NMR, and identified as O-$\alpha$-D-galactosyl-(1${\to}$2)-$\beta$-D-fructofuranoside. These results indicate that the main transfructosylation activity of the enzyme is to make nonreducing transferred products via a transfer of fructosyl residue to acceptor molecules having reducing group. When nonreducing sugars, such as methyl $\alpha$-D-glucoside and methyl $\alpha$-D-galactoside, were used as an acceptor, the transfer product was also formed in spite of the reducing group blocked with methyl group. The fact that no transfer product was formed with sugar alcohols as acceptors was suggested to be due to marked conformational difference of acceptors.

참고문헌 (18)

  1. Charnbert, R., G. Treboul, and R. Dedonder. 1974. Kinetic studies of levan sucrase of Bacillus subtilis. Eur. J. Biochem. 41: 285- 300 
  2. Fujita, K., N. Kuwahara, T. Tanimoto, K. Koizumi, M. Iizuka, N. Munamiura, K. Furuichi, and S. Kitahata. 1994. Chemical structures of hetero-oligosaccharides produced by Arthrobacter sp. K-1 $\beta$-fructofuranosidase. Biosci. Biotech. Biochem. 58: 239- 243 
  3. Hendry, G. A. F. and R. K. Wallace. 1993. The origin, distribution and evolutionary significance of fructans, pp. 119- 139. In: Suzuki M, Chatterton N. J. (eds.). Science and Technology of Fructans. Boca Raton: CRC Press 
  4. Kim, M. G., C. H. Kim, J. S. Lee, K. B. Song, and S. K. Rhee. 2000. Synthesis of methyl $\beta-D-fructoside$ catalyzed by levan sucrase from Rahnella aquatilis. Enz: Microb. Tech. 27: 646- 651 
  5. Ohtsuka, K., S. Hino, T. Fukushima, O. Ozawa, T. Kanematsu, and T. Uchida. 1992. Characterization of levansucrase from Rahnella aquatilis JCM-1683. Biosci. Biotechnol. Biochem. 56: 1373- 1377 
  6. Park, J. M., S. Y. Kwon, K. B. Song, J. W. Kwak, S. B. Lee, Y. W. Nam, J. S. Shin, Y. I. Park, S. K. Rhee, and K. H. Paek. 1999. Transgenic tobacco plants expressing the bacterial levansucrase gene show enhanced tolerance to osmotic stress. J. Microbiol. Biotechnol. 9: 213- 218 
  7. Pollock, C. J. and A. J. Cairns. 1991. Fructan metabolism in grasses and cereals. Ann. Rev. Plant Physiol. Plant Mol. Biol. 42: 77- 101 
  8. Song, E. K., H. Kim, H. K. Sung, and J. Cha. 2002. Cloning and characterization of a levanbiohydrolase from Microbacterium laevaniformans ATCC 15953. Gene 291: 45- 55 
  9. Tanaka, T., S. Yamamoto, and T. Yamamoto. 1981. Structures of hetero-oligosaccharides synthesized by levansucrase. J. Biochem. 90: 521-526 
  10. Kim, H., H.-E. Park, M.-J. Kim, H. G. Lee, J.-Y. Yang, and J. Cha. 2003. Enzymatic characterization of a recombinant levan sucrase from Rahnella aquatilis ATCC 15552. J. Microbiol. Biotechnol. 13: 230- 235 
  11. Park, H.-E., N. H. Park, M.-J. Kim, T. H. Lee, H. G. Lee, J.-Y. Yang, and J. Cha. 2003. Enzymatic synthesis offructosyl oligosaccharides by levansucrase from Microbacterium laevaniformans ATCC 15953. Enzyme Microb. Tech. 32: 820- 827 
  12. Meng, G. and K. Futterer. 2003. Structural framework of fructosyl transfer in Bacillus subtilis levansucrase. Nat. Struct. Biol. 10: 935- 941 
  13. Ananthalakshmy, V. K and P. Gunasekaran. 1999. Overproduction of levan in Zymomonas mobilis by using cloned sacB gene. Enzyme Microb. Technol. 25: 109- 115 
  14. Yanase, H., M. Iwata, R. Nakahigashi, K. Kita, and K. Tonomura. 1992. Purification, crystallization, and properties of the extracellular levan sucrase from Zymomonas mobilis. Biosci. Biotechnol. Biochem. 56: 1335- 1337 
  15. Vijn. I. and S. Smeekens. 1999. Fructan: More than a reserve carbohydrate? Plant Physiol. 120: 351- 359 
  16. Cairns, A. J. 1993. Evidence for the de novo synthesis of fructan by enzymes from higher plants; a reappraisal of the SST/FFT model. New Phytologist 123: 15- 24 
  17. Fujita, K., K. Hara, H. Hashimoto, and S. Kitahata. 1990. Purification and some properties of $\beta-fructofuranosidase$ I from Arthrobacter sp. K-1. Agric. Biol. Chem. 54: 913- 919 
  18. Chambert, R. and G. Gonzy1-Treboul. 1976. Levansucrase of Bacillus subtilis: Kinetic and thermodynamic aspects of transfructosylation processes. Eur. J. Biochem. 62: 55- 64 

이 논문을 인용한 문헌 (4)

  1. 2007. "" Journal of microbiology and biotechnology, 17(11): 1751~1757 
  2. 2007. "" Journal of microbiology and biotechnology, 17(5): 792~799 
  3. 2008. "" Journal of microbiology and biotechnology, 18(6): 1064~1069 
  4. 2009. "" Journal of microbiology and biotechnology, 19(10): 1153~1160 


원문 PDF 다운로드

  • ScienceON :
  • KCI :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일