[논문]BIOCHEMICAL MODEL AND MECHANISM FOR ACINETOBACTER NITRITE INHIBITION

Lee, Chan-Won; Weon, Seung-Yeon

doi:10.4491/eer.2005.10.1.022

BIOCHEMICAL MODEL AND MECHANISM FOR ACINETOBACTER NITRITE INHIBITION 원문보기

Environmental engineering research, v.10 no.1, 2005년, pp.22 - 30

Lee, Chan-Won , Weon, Seung-Yeon

Abstract ▼ AI-Helper

Nitrite accumulation is not unusual in batch processes such as sequencing batch reactor (SBR) with high-strength of ammonium or nitrate wastewaters. A possible mechanism of nitrite inhibition on Acinetobacter was depicted in a biochemical model, which the protonated species, nitrous acid form of nitrite, affects proton relating transport at the proton-pumping site crossing the cell membrane under unlimited carbon and phosphorus conditions. This effect exerts inhibition of phosphorylation under aerobic condition and yields low APT/ADP ratio, consequently decrease poly-P synthesis and phosphorus uptake from outside the cell in the model.

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

Although several microorganisms have been involved in EBPR processes, Acinetobacter species would be one of microorganisms to be always detected in the activated sludge of EBPR plants. The purpose of this research was to study the mechanism of nitrite inhibition to Acinetobater under aerobic condition to get better understandings for a relevant condition of nitrite build-up relating to biological phosphorus removal.

가설 설정

3. Poly-P synthesis pathway identified in a variety of organisms is via phosphorylation of the poly-P by ATP. Regulation of this pathway is via ATP/ADP ratio, therefore, inhibition of ATP synthesis yields low ATP/ ADP ratio, consequently decrease poly-P synthesis and phosphorus uptake as appeared in the model.

제안 방법

To investigate the effects of nitrite and phosphate on cell growth, the concentrations of nitrite were varied in different experiments. Biomass in terms of absorbance, pH, and temperature were monitored throughout each experiment.
25 g/L for unlimited carbon source. The biomass concentration measurements were made by spectrophotometer absorbance at a wavelength of 550 nm and were converted to cell biomass concentration using the cell biomass - spectrophotometer absorbance relationship. The experimental data has been well acceptably described with a linear model exhibiting a correlation coefficient of 0.
To determine the values of specific growth rate (p), inhibition tests were performed with various nitrite concentrations and the same initial phosphate concentration of 18.3 mg/L, and the data of In Xt/Xo was plotted against time for various nitrite concentrations. A typical specific growth rate (0 of Acinetobacter for various nitrite and FNA concentration is shown in Figure 5.

대상 데이터

Acinetobacter sp. (ATCC 11171) was used in this study.
The experimental set-up is consisted of eight batch reactors. The reactors were autoclaved and covered with lid but were not air tight.

이론/모형

2 mm pore size glass fiber filter (Whatman GF/C) as a prefilter. The concentration of nitrite (NO2-N) and ortho-phosphate (PO4-P) were determined using the Colorimetric (HACH NitriVer 2) and Ascorbic Acid methods, re- spectivelyl0). All analysis was in duplicate.

성능/효과

4. Thus, the results obtained in this study indicates that nitrite accumulate and pH is very important for biological phosphorus removal, specially when treating high strength of ammonium wastewater, because of nitrite inhibitory effect on poly-P bacteria and pH dependency of nitrite inhibitory form.
The results obtained in this study indicates that nitrite accumulate and pH is very important for biological phosphorus removal, specially when treating high strength of ammonium wastewater, because of nitrite inhibitory effect on poly-P bacteria and pH dependency of nitrite inhibitory form.

참고문헌 (29)

Fuhs, G. W. and Chen, M., 'Microbiological basis of phosphate removal in the activated sludge process for the treatment of wastewater,' Microb. Ecol., 2, 119-138 (1975)

상세보기
Ohtake, H., Takahashi, K., Tsuzuki, Y., and Toda, K., 'Uptake and release of phosphate by a pure culture of Acinetobacter calcoaceticus,' Water Res., 19, 1587-1594 (1985)

상세보기
Mino, T., van Loosdrecht, M. C. M., and Heijnen, J. J., 'Microbiology and biochemistry of the enhanced biological phosphate removal process,' Water Res., 32, 3193-3207 (1998)

상세보기
Auling, G., Pilz, F., Busse, H.-J., Karrasch, S., Streichan, M., and Schon, G., 'Analysis of the polyphosphate-accumulating microflora in phosphorus eliminating,anaerobic-aerobic activated sludge systems by using diaminopropane as a biomarker for rapid estimation of acinetobacter spp, Appl. Environ. Microbiol., 57, 3685-3692 (1991)
Jenkins, D. and Tandoi, V., 'The applied microbiology of enhanced biological phosphate removal accomplishment and needs,' Water Res., 25, 1471-1478 (1991)

상세보기
Zafiri, C., Kornaros, M., and Lyberatos, G., 'Kinetic modeling of biological phosphorus removal with a pure culture of acinetobacter sp. under aerobic, anaerobic and transient operating conditions,' Water Res., 33, 2769-2788 (1999)

상세보기
Wilderer, P. A, Jones, W. L., and Dau, U., 'Competition in denitrification systems affecting reduction rate and accumulation of nitrite,' Water Res., 21, 239-245 (1987)

상세보기
Meinhold, J., Arnold, E., and Issaacs, S., 'Effect of nitrite on anoxic phosphate uptake in biological phosphorus removal activated sludge,' Water Res., 33, 1871-1883 (1999)

상세보기
Weon, S.-Y., Lee, C.-W., Lee, S.-I., and Koopman, B., 'Nitrite inhibition of aerobic growth of Acinetobacter sp,' Water Res., 36, 4471-4476 (2002)

상세보기
American Public Health Association; American Water Works Association; and Water Environment Federation., Standard Methods for the Examination of Water and Wastewater. 19th Ed., Washington, D.C (1995)
Wentzel, M. C., Lotter, L. H., Loewenthal, R. E., Marais, G. v. R., 'Metabolic behavior of Acinetobacter spp. In enhanced biological phosphorus removal: a biochemical model,' Water SA., 12, 209-224 (1986)
Lotter, L. H., 'The role of bacterial phosphate metabolism in enhanced phosphorus removal from the activated sludge process,' Water Sci. Technol., 17, 127-138 (1984)
Kuba, T, W., van Loosdrecht, M.C.M., and Heijnen J. J., 'Effect of nitrate on phosphorus release in biological phosphorus removal systems,' Water Sci. Technol., 30, 263-269 (1994)

상세보기
Barker, P. S. and Dold P. L., 'Denitrification behavior in biological excess phosphorus removal activated sludge systems,' Water Res., 30, 769-780 (1996)

상세보기
Comeau, Y, Hall, K. J., Hancock, R. E. W., and Oldham, W. K., 'Biochemical model for enhanced biological phosphorus removal,' Water Res., 20, 1511-1521 (1986)

상세보기
Kern-Jespersen, J. P. and Henze, M., 'Biological phosphorus uptake under anoxic and aerobic conditions,' Water Res., 27, 617-624 (1993)

상세보기
Kuba, T., van Loosdrecht, M. eM., and Heijnen, J. J., 'Effect of cyclic oxygen exposure on the activity of denitrifying phosphorus removing bacteria,' Water Sci. Technol., 34, 33-40 (1996)

상세보기
Vlekke, G. J. K., Comeau, Y., and Oldham, W. K., 'Biological phosphorus removal from wastewater with oxygen nitrate in sequencing batch reactors,' Environmental Technology Letters 9, 791-796 (1988)

상세보기
Anthonisen, A. C., Loehr, R. C., Prakasam, T. B. S., and Srinarh, E. G., 'Inhibition of nitrification by ammonia and nitrous acid,' J. Water Pollut. Control Fed., 48, 835-852 (1976)

상세보기
Balmelle, B., Nguyen, K M., Caodeville, B., Cornier, J. C, and Deguin, A, 'Study of factors controlling nitrite build-up in biological process for water nitrification,' Water Sci. Technol., 26, 1017-1025 (1992)
Hanaki, K., Wantawin, C., and Ohgaki, S., 'Nitrification at low levels of dissolved oxygen with and without organic loading in a suspended-growth reactor,' Water Res., 24, 297-302 (1990)

상세보기
Bode, H., Seyfried, C., and Kraft, A., 'High-rate denitrification of concentrated nitrate wastewater,' Water Sci. Technol., 19, 163-174 (1987)

상세보기
Abeling, U. and Seyfried, C., 'Anaerobic-aerobic treatment of high-strength ammonium wastewater-nitrogen removal via nitrite,' Water Sci. Technol., 26, 1007-1015 (1992)
Glass, C., Silverstein, J., and Oh, J., 'Inhibition of denitrification in activated sludge by nitrite. Water Environ. Res., 69, 1086-1093 (1997)

상세보기
Sijbesma, W. F. H., Almeida, J. S., Reis, M. A. M., and Santos, H., 'Uncoupling effect of nitrite during denitrification by Pseudomonas fluorescens: An in Vivo PNMR study,' Biotechnol. Bioeng., 52, 176182 (1996)
Kim, H. J. and Pagilla, K. R., 'Competitive growth of Nocardia and acinetobacter under anaerobic/aerobic bath operation,' Water Res., 34, 2667-2674 (2000)

상세보기
Comeau, Y, Oldham, W. K., and Hall, K. J., 'Dynamics of carbon reserves in biological dephosphatation of wastewater,' Proceeding of an IAWPRC specialized conference in Rome on biological phosphate removal from wastewater. pp. 39-55 (1987)
Yarbrough, J. M., Rake, J. B., and Eagon, R. G., 'Bacterial inhibition effects of nitrite : inhibition of active transport, but not of group translocation, and of intracellular enzymes,' Appl. Environ. Microbiol., 39, 831-834 (1980)
Thomsen, J. K., Geest, T., and Cox, R. P., 'Mass Spectrometric studies of the effect of pH on the accumulation of intermediates in denitrification by paracoccus denitrificans,' Appl. Environ. Mocrobiol., 60, 2, 536 (1994)

원문 URL 링크

DOI : 10.4491/eer.2005.10.1.022 [무료]
대한환경공학회 : 저널
DBPia : 저널
AccessON : 저널

*원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다.

오픈액세스(OA) 유형

BRONZE

출판사/학술단체 등이 한시적으로 특별한 프로모션 또는 일정기간 경과 후 접근을 허용하여, 출판사/학술단체 등의 사이트에서 이용 가능한 논문

내보내기 메뉴

내보내기 구분

파일저장
인쇄
메일전송

구성항목

기본정보
상세정보

관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관

저장형식

Text(ASCII format)
Excel format
RefWorks Direct Export
RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley

메일정보

받는사람 (필수): @
보내는사람 (선택): @
제목
내용: KISTI 검색결과 이메일 서비스

안내

총 건의 자료가 검색되었습니다.

다운받으실 자료의 인덱스를 입력하세요. (1-10,000)

검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다.

데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요)

다운로드 파일은 UTF-8 형태로 저장됩니다.
파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오.

Text(ASCII format)
Excel format

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

AI-Helper ※ AI-Helper는 을 사용합니다.

AI-Helper

안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

연합인증