[논문]In vitro Conservation of Coleus forskohlii- an Endangered Medicinal Plant

Rajasekharan P.E.; Ambika S.R.; Ganeshan S.

In vitro Conservation of Coleus forskohlii- an Endangered Medicinal Plant 원문보기

Journal of plant biotechnology, v.7 no.2, 2005년, pp.135 - 141

Rajasekharan P.E. (In Vitro Conservation and Cryopreservation Laboratory, Division of Plant Genetic Resources, Indian Institute of Horticultural Research, Hessaraghatta Lake) , Ambika S.R. (Physiology laboratory, Department of Botany, Bangalore University) , Ganeshan S. (In Vitro Conservation and Cryopreservation Laboratory, Division of Plant Genetic Resources, Indian Institute of Horticultural Research, Hessaraghatta Lake)

Abstract ▼ AI-Helper

Protocols for in vitro conservation was developed for Coleus forskohlii. Plants maintained both in field served as explant source. Shoot tips and single node cuttings were used to optimize protocols for in vitro multiplication. MS basal medium supplemented with $0.54\;{\mu}M$ naphthalene acetic acid (NAA) and $8.87\;{\mu}M$ benzy-ladenine (BA) induced multiple shoots in shoot tips and nodes. Shoot multiplication was amplified with a gradual decrease of BA concentration, leading to its final omission after 4 months. Concomitant rooting on multiplication media enabled successful establishment extra vitrum. For in vitro conservation studies, experiments were carried out with 2-3 week maintained in vitro plants under standard and reduced culture conditions (SCC, RCC). In vitro plants could be successfully conserved in full strength MS medium (FMS) under SCC for 6 months without subculture with full potential to regenerate, producing viable shoots and nodes. The root production remained unaffected due to conservation, showing high rooting activity in mannitol and low temperature treatments. Preset low temperature (15 and $10^{\circ}C$) and reduction in media constituents does not appear to favour conservation, although the former accomplished conservation levels equal to (FMS) under SCC.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

forskohlii for 12 to 20 months at 25 ℃ without requiring any sub culturing, with no apparent differences observed when explants were regenerated without growth regulators. These studies however, are deficient in terms of a detailed analysis of the parameters that affect conservation, which has been carried o_니t in this study. From the results obtained, one can conclude that maximum conservation gain with extended storage duration of 6 months has been obtained with treatments involving FMS+ SCC, lowering the temperature or reducing media constituents appear_옹less gainful for conservation.

대상 데이터

15 ml of molten medium dispensed into sterile 25 X 150 cm cult_니re tubes. For each treatment, 10 replicates were used and all the experiments were repeated thrice. The cultures were incubated at 25 ± 2 ℃ with 50 to 55 % RH and a 16 h photoperiod, provided by cool * Philips' white fluorescent tubes with an intensity of 31.

데이터처리

The data were analyzed using factori_기CRD and ANOVA was performed. (Compton 1996).

성능/효과

4) Reducing subculture frequency and economize on material and humaneso_니rces. 5) parameters to guide as conservation indices were identified for recording observation; to estimate growth reduction and conservation gain among the treatments ind_니ced. These parameters were chosen as per IPGRI gnid_이ines (CIAT/ IPGRI 1994), modified to suit the species.
e to the fact that roots are the targeted part used in medicine. After 6 months, there has been a significant increase in root number in FMS+ SCC+ M treatment compared to other conservation treatments investigated. The results substantiate that the conservation treatments does not reduce root production, but a substantial increase in root number in mannitol treatment co_니Id be due to associated stress factors.
These studies however, are deficient in terms of a detailed analysis of the parameters that affect conservation, which has been carried o_니t in this study. From the results obtained, one can conclude that maximum conservation gain with extended storage duration of 6 months has been obtained with treatments involving FMS+ SCC, lowering the temperature or reducing media constituents appear_옹less gainful for conservation.

참고문헌 (28)

Anonymous (1999) The key role of forestry sector in conserving India's medicinal plants conceptual and operational features. FRLHT, Bangalore
Ashmore SE (1997) Status report on the development and application of in vitro techniques for the conservation and use of plant genetic resources. IPGRI, Rome
Bhat SR, Chandel KPS (1993) In Vitro conservation of Musa germ plasm. Effects of mannitol and temperature on growth and storage. J Horticultural Sci 68 841-846

상세보기
Chandel KPS and Sharma N (1992) Conservation of diversity in medicinal plants species through in vitro technology. Abstr Symposium on Frontiers in Plant Biotechnology, Biotechnology center IARI, New Delhi
Chandel KPS, Shukla G, Sharma N (1996) Biodiversity in medicinal and aromatic plants in India. NBPGR, New Delhi, India
Compton ME (1996). Statistical analysis of plant tissue culture data. In: Trigeano RN, Gray DJ (eds), Plant Tissue Culture Concepts and Laboratory Exercises. CRC Press, Boca Raton
Engelmann F (1997) In vitro conservation methods. In: FordLloyd BV, Newbury HJ, Callow JA (eds), Biotechnology and Plant Genetic Resources Conservation and use. CABI, Oxon, UK
Grout B (1995) Genetic Preservation of Plant Cells in vitro. Springer Verlag Laboratory Manual, Springer Verlag, Berlin
Gupta R (1988) Genetic resources of Indian Medicinal Plants J Plant Genet Resources 1:90-102
IPGRI/CIAT(1994) Establishment and operation of pilot in vitro active genebank. Report of a IPGRI/CIAT collaborative project using Cassava (Manihot esculenta Cranz) as a model IPGRI, Rome
Jarrret RL, Gawell N (1991) Chemical and environmental growth regeneration in sweet potato (Ipomoea batatus Lam.) in vitro. Plant Cell Tiss Org Cui 25: 153-160
Kozaki T (1991) Micropropagation under photoautotrophic conditions In: Debergh PE, Zimmerman RHZ (eds), Micropropagation Technology and application. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp447-469
Kubota C, Kozai T (1995) Low temperature storage of transplants at the light compensation point Air temperature and light intensity for growth suppression and quality preservation. Scientia Hort 64: 1-9

상세보기
Mandai BB, Tyagi RK, Pandey R, Sharma N, Agrawal A (2000) Conservation of germplasm of agri-horticultural crops at NBPGR- An over view. In: Razdan MK, Cocking EC (eds) Conservation of PGR in vitro. Vol. 2, Application and Limitations. Oxford and IBH Publishing Co, Pvt Ltd, New Delhi
McCowen BH (2000) Recalcitrance of woody and herbaceous perennial plants: Dealing with genetic predeterminism. In Vitro Cell Dev Bioi-Plant 36: 14

상세보기
Murashige T, Skoog F(1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473-497

상세보기
Pruski K, Kozai T, Lewis T, Astatkie T, Nowak J (2000) Sucrose and light effects on in vitro cultures of potato, chokecherry and sakatoon berry during low temperature storage Plant Cell tiss Org Cult 63: 215-221
Rajasekharan PE, Ganeshan S (2002) Conservation of medicinal plant biodiversity - an Indian perspective. J Med Aroma Plant Sci 24: 132-147
Rajora OP, Mossier A (2000) Challenges and opportunities for conservation of forest genetic resources Euphytica 118: 197-21
Roca WM, Chavez R, Martin ML, Arias DI, Mafia G, Reyes R (1989). In vitro methods of germ plasm conservation. Genome 31:813-817

상세보기
Sen J, Sharma AK (1991) In vitro propagation of Coleus forskohlii Briq. For forkohlin synthesis. Plant Cell Rep 9:696-698

상세보기
Sharma N, Chandel KPS (1995) In vitro conservation of threatened plants of Medicinal Importance. Indian Journal of Plant Genetic Resources 8: 107-112
Sharma N, Chandel KPS, Srivatava VK (1991). In vitro propagation of Coleus forskohlii Briq. A threatened medicinal plant Plant Cell Rep 10:67-70

상세보기
Sharma N, Chandel KPS (1992) Effects of ascorbic acid on axillary shoot induction on Tylophora indica (Burm. f.) Merill. Plant Cell Tiss Org cult 29: 109-113

상세보기
Suryanarayanan M, Pai JS (1998) Studies in micropropagation of Coleus forskohlii J Medicinal Aromatic Plant Sci 20:379-382
Vishwakarmakarma RA, Tyagi BR Ahmed B, Hussain A (1988) Variations in forkohlin content in roots of Coleus forkohlii. Planta Med 54: 471-472

상세보기
Wilson SB, Rajapakse NC, Young RE (2000). Media composition and light affect storability and poststorage recovery of micropropagated Hosta plants. Hort Sci 35: 1159-1162
Yanagihara H, Sakata R, Shoyanna Y, Murakami H (1995) Relationship between the content of forskohlin and Growth environment in clonally propagated Coleus forskohlii Briq. Biotronics 24:1

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

In vitro Conservation of Coleus forskohlii- an Endangered Medicinal Plant 원문보기

Abstract ▼ AI-Helper

주제어

AI 본문요약
AI-Helper

제안 방법

대상 데이터

데이터처리

성능/효과

참고문헌 (28)

이 논문을 인용한 문헌

관련 콘텐츠

원문 보기

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

In vitro Conservation of Coleus forskohlii- an Endangered Medicinal Plant 원문보기

Abstract ▼ AI-Helper

주제어

AI 본문요약 엑셀 다운로드 AI-Helper

제안 방법

대상 데이터

데이터처리

성능/효과

참고문헌 (28)

이 논문을 인용한 문헌

관련 콘텐츠

원문 보기

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

AI 본문요약
AI-Helper