$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Abstract

Many cloning vectors in which cDNAs can be inserted to the sense orientation have been developed. Uni-ZAP XR vector (Stratagene) should contain clones that are oriented to sense direction with respect to T3 RNA polymerase primer. Unexpectedly, large portions of cDNAs in Chinese cabbage cDNA library showed unusual insertions, antisense orientation and a hybrid of two different clones. Using two clones, 4H03 and 53-B10, derived from different cDNA libraries, we proposed and demonstrated the possibility of unusual-construct formation by in vitro translation and northern blot analysis. The 4H03 clone was inserted with inverse direction, and its transcript and translation product could be produced by T7 RNA polymerase, indicating that this clone is definitely inserted into inverse orientation. The 53-B10 that contains two independent genes was turned out to be a hybrid in which two genes are inserted to opposite direction each other. All unusual constructs might be due to the presence of small fragments of DNA, like adapter. However, the mechanism underlined the formation of unusual constructs is still remain to be solved.

저자의 다른 논문

참고문헌 (11)

  1. Baltimore D (1970) RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature 226: 1209-1211 
  2. Rougeon F, Kourilsky P, Mach B (1975) Insertion of a rabbit ${\beta}$ -globin gene sequence into an E. coli plasmid. Nucleic Acids Res 2: 2365-2378 
  3. Ross J, Aviv H, Scolnick E, Leder P (1972) In vitro synthesis of DNA complementary to purified rabbit globin mRNA (RNA-dependent DNA polymerase-reticulocyte-hemoglobindensity grdient centrifugation-oligo (dT) primer). Proc Natl Acad Sci 69: 264-268 
  4. Krug MS, Berger SL (1987) First-strand cDNA synthesis primed with oligo (dT). Method Enzymol 152: 316-325 
  5. Sambrook J, Russell DW (2001) Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor Press, Cold Spring Harbor, New York 
  6. Rabbius TH (1976) Bacterial cloning of plasm ids carrying copies of rabbit globin messenger RNA. Nature 260: 221-225 
  7. Kretz PL, Reid CH, Greener A, Short JM (1989) Effect of lambda packaging extract mer restriction activity on DNA cloning. Nucleic Acids Res 17: 5409 
  8. Temin HM, Mizutani S (1970) RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature 226: 1211-1213 
  9. Verma IM, Temple GF, Fan H, Baltimore D (1972) In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA. Nat New Biol 235: 163-167 
  10. Bank A, Terada M, Metafora S, Dow L, Marks PA (1972) In vitro synthesis of DNA components of human genes for globins. Nat New Biol 235: 167-169 
  11. Short JM, Fernandez JM, Sorge JA, Huse ED (1988) ${\lambda}$ ZAP: a bacteriophage ${\lambda}$expression vector with in vivo excision properties. Nucleic Acids Res 16: 7583-7600 

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음

원문보기

원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일