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Determination of Water Content in Skin by using a FT Near Infrared Spectrometer 원문보기

Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea, v.28 no.4, 2005년, pp.458 - 462  

Suh Eun-Jung (College of Pharmacy, Dongduk Women's University) ,  Woo Young-Ah (College of Pharmacy, Dongduk Women's University) ,  Kim Hyo-Jin (College of Pharmacy, Dongduk Women's University)

Abstract AI-Helper 아이콘AI-Helper

The water content of skin was determined using a FT near infrared (NIR) spectrometer. NIR diffuse reflectance spectra were collected from hairless mouse, in vitro, and from human inner arm, in vivo. It was found that the variation of NIR absorbance band 1450 nm from OH vibration of water and 1940 nm...

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제안 방법

  • The prediction set consisted of samples that were not used for the calibration set. Each developed NIR model was eval니ated as the standard errors of calibration (SEC) for the calibration set and the standard errors of prediction (SEP) for the prediction set.
  • In this study, the rapid and nondestructive method for determination of the water content in SC was developed by using a FT NIR spectrometer with a fiber-optics probe.
  • All of spectra were separated in to two sets for the development of PLS modeling and the evaluation of the PLS models. PLS regression was performed in the same method as used for the hairless mouse skin moisture calibration, Three regions, 1130-1830 nm, 1200-1670 nm, and 1380-1600 nm, were used to find a robust model. Also, to enhance the spectral features, derivative tech-niques were used.
  • The samples were divided into a calibration set for modeling and a prediction set for the evaluation of the developed model. The prediction set consisted of samples that were not used for the calibration set.
  • In vitro or in vivo, they showed the good correlation between water content and NIR absorbance in addition to advantages of NIR analysis over other measurements (Walling and Dabney, 1989; Martin, 1998). These studies were performed with conventional NIR instruments with integrating sphere or moving grating, which are hard to move and handle for practical use. According to recent ov이view on MR spectroscopy of skin (Heise, 2000; Kumar and Schmitt, 1997), diffuse reflectance spectra were recorded with the use of optical fiber probe and were valuably used for clinical diagnostics.

대상 데이터

  • Dorsal skin from two hairless mice (8 weeks) was used. The epidermis parts were separated from the dermal tissues and were cut into 8 pieces.
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참고문헌 (19)

  1. Caspers, P. J., Lucassen, G. W., Carter, E. A., Bruining, H. A., and Puppels, G. J., In-vivo confocal raman microscopy of the skin: Non-invasive determination of molecular concentration profiles. J. Invest. Dermatol., 116, 434-442 (2001) 

  2. Courage W., Hardware and measuring principle: Corneometer, In Elsner, P., Berardesca, E., and Maibach, H. I. (Eds.). Bioengineering of the skin: Water and the Stratum Corneum. CRC press, Boca Raton, Chapter 14, (1994) 

  3. Egawa, M., Fukuhara, T., Takahashi, M., and Ozaki, Y., Determining Water Content in Human Nails with a Portable Near- Infrared Spectrometer. Appl. Spectrosc., 57, 473-478 (2003) 

  4. Heise, H. M., Clinical applications of near- and mid- infrared spectroscopy, In Gremlich, H. U., and Yan, B. (Eds.). Infrared and raman spectroscopy of biological materials. Marcel Dekker, New York, (2000) 

  5. Kumar, G. and Shmitt, J. M., Optimal probe geometry for nearinfrared spectroscopy of biological tissue. Appl. Optics, 36, 2286-2293 (1997) 

  6. Libnau, F. O., Kvalheim, O. M., Christy, A. A., and Toft, J., Infrared spectra of water in the NIR and MIR region. Vib. Spectrosc., 7, 243-254 (1994) 

  7. Lucassen, G. W., Veen, G. N. A., and Jansen, J. A. J., Band analysis of hydrated human skin stratum corneum attenuated total reflectance fourier transform intrared spectra in vivo. J. Biomedical Optics, 3, 267-280 (1998) 

  8. Martin, K. A., Direct measurement of moisture in skin by NIR spectroscopy. J. Soc. Cosmet. Chem., 44, 249-261 (1993) 

  9. Martin, K., In vivo measurements of water in skin by nearinfrared reflectance. Appl. Spectrosc., 52, 1001-1007 (1998) 

  10. Martinsen, O. G., Grimnes, S., and Karlsen, J., Electrical methods for skin moisture assessment. Skin Pharmacol., 8, 237-245 (1995) 

  11. Obata, M. and Tagami, H., A rapid in vitro test to assess skin moisturizers. J. Soc. Cosmet. Chem., 41, 235-241 (1990) 

  12. Potts, R. O., Stratum corneum hydration : Experimental techniques and interpretations of results. J. Soc. Cosmet. Chem., 37, 9-33 (1986) 

  13. Prasch, T. H., Knubel, G., Schmidt-Fonk, K., Ortanderl, S., Nieveler, S., and Forster, T. H., Infrared spectroscopy of the skin: influencing the stratum corneum with cosmetic products. Int. J. Cos. Sci., 22, 371-383 (2000) 

  14. Rigal, J., Losch, M. J., Bazin, R., Camus, C., Sturelle, C., Descamps, V., and Leveque, J. L., Near-infrared spectroscopy: A new approach to the characterization of dry skin. J. Soc. Cosmet. Chem., 44, 197-209 (1993) 

  15. Salter, D. C., Examination of stratum corneum hydration state by electrical methods. Curr. Probl. Dermatol., 26, 38-47 (1998) 

  16. Sowa, M. G., Payette, J. R., and Mantsch, H. H., Near-infrared spectroscopic assessment of tissue hydration following surgery. J. Surg. Res., 86, 62-69 (1999) 

  17. Tagami, H., Quantitative measurements of water concentration of the stratum corneum in vivo by high-frequency current. Acta Derm. Venerol. Suppl. (Stockh.), 185, 29-33 (1994) 

  18. Walling, P. L. and Dabney, J. M., Moisture in skin by nearinfrared reflectance spectroscopy. J. Soc. Cosmet. Chem., 40, 151-171 (1989) 

  19. Wichrowski, K., Sore, G., and Khaiat, A., Use of infrared spectroscopy for in vivo measurement of the stratum corneum moisturization after application of cosmetic preparations. Int. J. Cos. Sci., 17, 1-11 (1995) 

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