[논문][ <tex>$Ce^{4+}$</tex> ]-Stimulated Ion Fluxes Are Responsible for Apoptosis and Taxol Biosynthesis in Suspension Cultures of Taxus Cells

Li Jing-Chuan; Ge Zhi-Qiang; Yuan Ying-Jin

doi:10.1007/bf02932579

[ $Ce^{4+}$ ]-Stimulated Ion Fluxes Are Responsible for Apoptosis and Taxol Biosynthesis in Suspension Cultures of Taxus Cells 원문보기

Biotechnology and bioprocess engineering : Bbe, v.10 no.2, 2005년, pp.109 - 114

Li Jing-Chuan (Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University) , Ge Zhi-Qiang (Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University) , Yuan Ying-Jin (Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University)

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Ion fluxes across the plasma membrane activated by 1 mM $Ce^{4+}$, cell apoptosis and taxol biosynthesis in suspension cultures of Taxus cusp/data were studied. The extracellular pH sharply decreased upon the addition of 1 mM $Ce^{4+}$, then increased gradually and exceeded the initial pH value over a time period of 12 h. The extracellular $Ca^{2+}$ concentration decreased within the first 3 h after the addition of $Ce^{4+}$, then gradually decreased to one third of initial value in control at about 72 h and remained unchanged afterwards. Experiments with an ion channel blocker and a $Ca^{2+}$-channel blocker indicated that the dynamic changes in extracellular pH and the $Ca^{2+}$ concentration resulted from the $Ce^{4+}$-induced activation of W uptake and $Ca^{2+}$ influx across the plasma membrane via ion channels. A pretreatment of the ion channel blocker initiated $Ce^{4+}$-treated cells to undergo necrosis, and the prior addition of the $Ca^{2+}$-channel blocker inhibited $Ce^{4+}$-induced taxol biosynthesis and apoptosis. It is thus inferred that W uptake is necessary for cells to survive a $Ce^{4+}$-caused acidic environment and is one of the mechanisms of $Ce^{4+}$-induced apoptosis. Furthermore, the $Ca^{2+}$ influx across the plasma membrane mediated both the $Ce^{4+}$-induced apoptosis and taxol biosynthesis.

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Ion fluxes over the plasma membrane are one of the early elements of these receptor-mediated responses and they play an essential role in an oxidative burst, the activation of defense-related genes and the accumulation of phytoalexin, In addition, both secondary metabolite biosynthesis and cell apoptosis have been recognized as the late elements of plant defense responses to elicitors [10]. In this work, the effects of Ce4+ on ion (H+ and Ca2+) fluxes across the plasma membrane were investigated in suspension cultures of Taxus cells by analysis of the dynamical changes of extracellular pH and Ca2+ concentration. Experiments with an ion channel blocker and Camchannel blocker were also conducted to shed light on the mechanism of action of Ce4+ on Taxol biosynthesis and cell apoptosis.

참고문헌 (27)

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[ $Ce^{4+}$ ]-Stimulated Ion Fluxes Are Responsible for Apoptosis and Taxol Biosynthesis in Suspension Cultures of Taxus Cells 원문보기

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[ $Ce^{4+}$ ]-Stimulated Ion Fluxes Are Responsible for Apoptosis and Taxol Biosynthesis in Suspension Cultures of Taxus Cells 원문보기

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