Wang Wei
(State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology)
,
Zhao Zhen-Jiang
(Shanghai Key Laboratory of Chemical Biology, Institute of Pesticides and Pharmarceuticals, East China University of Science and Technology)
,
Xu Yufang
(Shanghai Key Laboratory of Chemical Biology, Institute of Pesticides and Pharmarceuticals, East China University of Science and Technology)
,
Qian Xu hong
(Shanghai Key Laboratory of Chemical Biology, Institute of Pesticides and Pharmarceuticals, East China University of Science and Technology)
,
Zhong Jian-Jiang
(State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology)
A series of fluorine and hydroxyl containing jasmonate derivatives, which were chemically synthesized in our institute, were investigated for their effects on the biosynthesis and heterogeneity of ginsenosides in suspension cultures of Panax notoginseng cells. Com-pared to the control (without addit...
A series of fluorine and hydroxyl containing jasmonate derivatives, which were chemically synthesized in our institute, were investigated for their effects on the biosynthesis and heterogeneity of ginsenosides in suspension cultures of Panax notoginseng cells. Com-pared to the control (without addition of elicitors), $100{\mu}M$ of each of the jasmonate was added on day 4 to the suspension cultures of P. notoginseng cells. It was observed that, jasmonates greatly enhanced the ginsenoside content and the ratio of Rb group to Rg group (i.e. $(Rb_1\;+\;Rd)/(Rg_1\;+\;Re)$ in the P. notoginseng cells. Some of the synthetic jasmonates, such as pentafluoropropyl jasmonate (PFPJA), 2-hydroxyethyl jasmonate (HEJA) and 2-hydroxye-thoxyethyl jasmonate (HEEJA), could promote the ginsenoside content to $2.55\;\pm\;0.11,\;3.65\;\pm\;0.13\;and\;2.94\;\pm\;0.06$mg/100 mg DW, respectively, compared to that of $0.64\;\pm\;0.06$mg/100 mg DW for the control and $2.17\;\pm\;0.04$ mg/100 mg DW by the commercially available methyl jasmonate (MJA); and they could change the respective Rb:Rg ratio to $1.60\;\pm\;0.04,\;1.87\;\pm\;0.01\;and\;1.56\;\pm\;0.05$, compared to that of $0.47\;\pm\;0.01$ for the control and $1.42\;\pm\;0.06$ by MJA. The results suggest that suitable esterification of MJA with fluorine or hydroxyl group could in-crease the elicitation activity to induce plant secondary metabolism. The information obtained from this study is useful for hyper-production of heterogeneous products by plant cell cultures.
A series of fluorine and hydroxyl containing jasmonate derivatives, which were chemically synthesized in our institute, were investigated for their effects on the biosynthesis and heterogeneity of ginsenosides in suspension cultures of Panax notoginseng cells. Com-pared to the control (without addition of elicitors), $100{\mu}M$ of each of the jasmonate was added on day 4 to the suspension cultures of P. notoginseng cells. It was observed that, jasmonates greatly enhanced the ginsenoside content and the ratio of Rb group to Rg group (i.e. $(Rb_1\;+\;Rd)/(Rg_1\;+\;Re)$ in the P. notoginseng cells. Some of the synthetic jasmonates, such as pentafluoropropyl jasmonate (PFPJA), 2-hydroxyethyl jasmonate (HEJA) and 2-hydroxye-thoxyethyl jasmonate (HEEJA), could promote the ginsenoside content to $2.55\;\pm\;0.11,\;3.65\;\pm\;0.13\;and\;2.94\;\pm\;0.06$mg/100 mg DW, respectively, compared to that of $0.64\;\pm\;0.06$mg/100 mg DW for the control and $2.17\;\pm\;0.04$ mg/100 mg DW by the commercially available methyl jasmonate (MJA); and they could change the respective Rb:Rg ratio to $1.60\;\pm\;0.04,\;1.87\;\pm\;0.01\;and\;1.56\;\pm\;0.05$, compared to that of $0.47\;\pm\;0.01$ for the control and $1.42\;\pm\;0.06$ by MJA. The results suggest that suitable esterification of MJA with fluorine or hydroxyl group could in-crease the elicitation activity to induce plant secondary metabolism. The information obtained from this study is useful for hyper-production of heterogeneous products by plant cell cultures.
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대상 데이터
Ltd (Ibkyo, Japan). Silica gel (200~400 mesh, 60 A, for column chromatography) was obtained from Aldrich. Organic solvents were obtained from commercial suppliers and were of the highest purity available; they were dried over 3 A molecular sieves for at least 48 h prior to use.
데이터처리
Each experiment was done in triplicate and 3 different sets of experiments reproduced the same result. Data were analyzed by one-way analysis of variance (ANOVA). Means of an experiment were analyzed using Tukey's honestly significant difference multiple-comparison test with a family error rate of 0.
Data were analyzed by one-way analysis of variance (ANOVA). Means of an experiment were analyzed using Tukey's honestly significant difference multiple-comparison test with a family error rate of 0.05. All differences were significant unless otherwise indicated.
a, b, c. d, e, f, g, h Means with the same letter, all noted in a single column are not significantly different according to TukeyJs honestly significant difference multiple-comparison test with a family error rate of 0.05.
성능/효과
For the hydroxyl containing jasmonates studied, 2-hydroxyethyl jasmonate (HEJA) and 2 - hydroxy ethoxy ethyl jasmonate (HEEJA) could enhance ginsenoside biosynthesis and change the heterogeneity of different ginsenosides more efficiently than MJA. Among them, HEJA had the highest stimulatory activity on ginsenoside biosynthesis, and the content of total ginsenosides and the Rb/Rg ratio on day 13 increased to about 70 and 30%, respectively when compared to MJA. In cell cultures of T.
후속연구
In other facet, till date, little information is available about the detailed elicitation mechanism of synthetic jasmonates. The relationship between the chemical structure of jasmonate elicitors and their stimulating activity needs further investigation towards rational design and synthesis of more potent elicitors.
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