Bacterial cellulose (BC), which is produced by some bacteria, has unique structural, functional, physical and chemical properties. Thus, the mass production of BC for industrial application has recently attracted considerable attention. To enhance BC production, two aspects have been considered, nam...
Bacterial cellulose (BC), which is produced by some bacteria, has unique structural, functional, physical and chemical properties. Thus, the mass production of BC for industrial application has recently attracted considerable attention. To enhance BC production, two aspects have been considered, namely, the engineering and genetic viewpoints. The former includes the reactor design, nutrient selection, process control and optimization; and the latter the cloning of the BC synthesis gene, and the genetic modification of the speculated genes for higher BC production. In this review, recent advances in BC production from the two viewpoints mentioned above are described, mainly using the bacterium Gluconacetobacter xylinus.
Bacterial cellulose (BC), which is produced by some bacteria, has unique structural, functional, physical and chemical properties. Thus, the mass production of BC for industrial application has recently attracted considerable attention. To enhance BC production, two aspects have been considered, namely, the engineering and genetic viewpoints. The former includes the reactor design, nutrient selection, process control and optimization; and the latter the cloning of the BC synthesis gene, and the genetic modification of the speculated genes for higher BC production. In this review, recent advances in BC production from the two viewpoints mentioned above are described, mainly using the bacterium Gluconacetobacter xylinus.
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제안 방법
Based on these previously obtained data, RSM was used to predict the levels of the four factors, fructose, corn steep liquor (CSL), dissolved oxygen (DO) and agar concentrations. A total of 27 experimental runs, with combinations of each factor, were carried out in a 10-L stirred-tank reactor, and a three-dimensional response surface generated to determine the effect of the factors and the optimum concentrations of each factor for maximum BC production. The predicted results showed an optimum BC production of 14.
The addition of water-soluble polysaccharides, like acetan and agar, enhanced BC productivity [40-42]. Based on these previously obtained data, RSM was used to predict the levels of the four factors, fructose, corn steep liquor (CSL), dissolved oxygen (DO) and agar concentrations. A total of 27 experimental runs, with combinations of each factor, were carried out in a 10-L stirred-tank reactor, and a three-dimensional response surface generated to determine the effect of the factors and the optimum concentrations of each factor for maximum BC production.
대상 데이터
intensively investigated BC production using a 50-L internal-loop airlift reactor [40, 45-47]. The reactor was equipped with a draft tube (134 mm inner diameter x 800 mm height), with a diameter ratio of the downcomer to the riser of 0.58. Air or oxygen-fortified air was supplied through the riser from gas spargers set at the bottom of the reactor, and the medium circulated by only the air pressure.
The energy consumption was compared between the airlift reactor supplied with both 0, 1% agar and oxygen- fortified air and a stirred-tank reactor. The same CSL- Fru medium and the strain A. xylinum BPR2001 were used in the two reactors. In the airlift reactor, the total energy was estimated as 43 kW with energy efficiency for 1 g/L BC production of 0.
성능/효과
A total of 27 experimental runs, with combinations of each factor, were carried out in a 10-L stirred-tank reactor, and a three-dimensional response surface generated to determine the effect of the factors and the optimum concentrations of each factor for maximum BC production. The predicted results showed an optimum BC production of 14.3 g/L under the condition of 4.99% fructose, 2.85% CSL, 28.3% DO and 0.38% agar concentrations. Under the optimized culture conditions, improvement in BC production, 14.
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