• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보


A DNA vaccine methodology using eukaryote expression vectors to produce immunizing proteins in the vaccinated hosts is a novel approach to the development of vaccine and immuno-therapeutics, and it has achieved considerable success over several infectious diseases and various cancers. To further enhance its efficiency, attempts were made to develop novel plasmid vectors containing multiple immunostimulatory CpG motifs, for rapid and strong immune response. First, a 2.9 kb compact plasmid vector (pVAC), containing CMV promoter, polycloning site, BGH poly(A) terminator, ampicillin resistance gene and pBR322 origin was constructed. A pVAC-hEPO was also constructed, which contained a human erythropoietin gene, for evaluating the transfection efficiency of naked plasmid DNA both in vitro and in vivo. To examine the adjuvant effect of multi-CpG motifs on naked plasmid DNA, 22 and 44 enriched and unmethylated CpG motifs were introduced into pVAC to generate pVAC-ISS1 and pVAC-ISS2, respectively. $100{\mu}g$ of pSecTagB, pVAC, pVAC-ISS1 or pVAC-ISS2 were each injected intramuscularly into the tibilias anterior muscle of Balb/c mice. The level of interleukin-6 induced in the mice injected with pVAC-ISS1 and pVAC-ISS2 were significantly elevated after 12 hours, which were almost 2 and 2.5 times higher than that in the mice injected with pSecTagB, respectively. These results suggest that DNA vaccine plasmids with enriched CpG motifs can induce rapid secretion of interleukin-6 by lymphocytes. In conclusion, these vectors can contribute to the development of adjuvant-free DNA vaccinations against infectious diseases and various cancers.

저자의 다른 논문

참고문헌 (22)

  1. Herrera, A. B., E. G. Rodriguez, T. Hernandez, B. Sandez, and C. A. Duarte (2000) A family of compact plasmid vectors for DNA immunization in humans. Biochem Biophys. Res. Commun. 279: 548-551 
  2. Tripathy, S. K., E. C. Svensson, H. B. Black, E. Goldwasser, M. Margalith, P. M. Hobart, and J. M. Leiden (1996) Long-term expression of erythropoietin in the systemic circulation of mice after intramuscular injection of a plasmid DNA vector. Proc. Natl. Acad. Sci. USA 93: 10876-10880 
  3. Mennuni, C., F. Calvaruso, I. Zampaglione, G. Rizzuto, D. Rinaudo, E. Dammassa, G. Ciliberto, E. Fattori, and N. La Monica (2002) Hyaluronidase increases electrogene transfer efficiency in skeletal muscle. Hum. Gene Ther. 13: 355-365 
  4. Y. Perrie, P. M. Frederik, and G. Gregoriadis (2001) Liposome- mediated DNA vaccination: The effect of vesicle composition. Vaccine 19: 3301-3310 
  5. Lindsay, W., R. Fernando, Z. Jie, and E. H. Daniel (1999) DNA immunization: Mechanistic studies. Vaccine 17: 1612-1619 
  6. Nicole, A., R. Doria, and L. H. Nancy (2003) DNA vaccine strategies: Candidates for immune modulation and immunization regimens. Methods 31: 207-216 
  7. Benjamin, L. E. and B. Franklin (1999) Regulation of the erythropoietin gene. Blood 94: 1864-1877 
  8. Klinman, D. M., G. Yamshchikov, and Y. Ishgatsubo (1997) Contribution of CpG motifs to the immunogenicity of DNA vaccines. J. Immunol. 158: 3635-3639 
  9. Harriet, L. and C. A. T. Celia (1997) DNA vaccines. Semin. Immunol. 9: 271-283 
  10. Krieg, A. M., A. K. Yi, S. Matson, T. J Waldschmidt, G. A. Bishop, R. Teasdale, G. A. Koretzky, and D. M. Klinman (1995) CpG motifs in bacterial DNA trigger direct B-cell activation. Nature 374: 546-549 
  11. Yoshtsugu, K., X. Ke-Qin, O. Takaki, H. Kenji, O. Tomohiro, S. Kaori, O. Tomomi, H. Yuka, J. Nao, N. Masatoshi, K. Dennis, and O. Kenji (2002) Adjuvant effect of multi- CpG motifs on an HIV-1 DNA vaccine. Vaccine 20: 2857- 2865 
  12. Kendall, R. G. (2001) Erythropoietin. Clin. Lab. Haematol. 23: 71-80 
  13. Lee, H. S., J. H. Jeong, J. H. Lee, and T. G. Park (2001) Enhancing transfection efficiency using polyethylene glycol grafted polyethylenimine and fusogenic peptide. Biotechnol. Bioprocess Eng. 6: 269-273 
  14. Ronald, K. S. (2000) The role of CpG motifs in immunostimulation and gene therapy. Adv. Drug Deliv. Rev. 44: 119-134 
  15. Mir, L. M., M. F. Bureau, J. Gehl, R. Rangara, D. Rouy, J. M. Caillaud, P. Delaere, D. Branellec, B. Schwartz, and D. Scherman (1999) High-efficiency gene transfer into skeletal muscle mediated by electric pulses. Proc. Natl. Acad. Sci. USA 96: 4262-4267 
  16. Hele, T., C. Maripat, R. Mark, and R. Eyal (1998) Gene vaccination: Plasmid DNA is more than just a blueprint. Immunol. Today 19: 89-97 
  17. Yi, A. K., D. M. Klinman, T. L. Martin, S. Matson, and A. Krieg (1996) Rapid immune activation by CpG motifs in bacterial DNA: Systemic induction of IL-6 transcription through an antioxidant-sensitive pathway. J. Immunol. 157: 5394-5402 
  18. Duchen, L. W., B. J. Excell, R. Patel, and B. Smith (1974) Changes in motor end-plates resulting from muscle fiber necrosis and regeneration: A light and electron microscopic study of the effects of the depolarizing fraction (cardiotoxin) of Dendroaspis jamesoni venom. J. Neurol. Sci. 21: 391-417 
  19. Klinman, D. M., J. Conover, J. M. Leiden, A. S. Rosenberg, and J. M. Sechler(1999) Safe and effective regulation of hematocrit by gene gun administration of an erythropoietin- encoding DNA plasmid. Hum. Gene Ther. 10: 659-665 
  20. Kim, Y. J., T. W. Kim, H. S. Chung, I. C. Kwon, and S. Y. Jeong (2001) Counterion effects on transfection activity of cationic lipid emulsion. Biotechnol. Bioprocess Eng. 6: 279-283 
  21. Shin, S., T. Fumihiko, X. Ke-Qin, I. Norihisa, and O. Kenji (2003) Technical issues in construction of nucleic acid vaccines. Methods 31: 199-206 
  22. Klinman, D. M. and A. K. Yi (1996) CpG motifs present in bacterial DNA rapidly induce lymphocytes to secrete interleukin 6, interleukin 12, and interferon $\gamma$. Proc. Natl. Acad. Sci. USA 93: 2879-2883 

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음


원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일