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논문 상세정보


Phytochelatin (PC) is involved in the detoxification of harmful, non-essential heavy metals and the homeostasis of essential heavy metals in plants. Its synthesis can be induced by either cadmium (Cd) or copper (Cu), and can form stable complexes with either element. This might suggest that PC has an important role in determining plant tolerance to both. However, this is not clearly apparent, as evidenced by a PC-deficient and Cd-sensitive Arabidopsis mutant (cad1-3) that shows no significant increase in its sensitivity to copper. Therefore, we investigated whether the mechanism for Cu tolerance differed from that for Cd by analyzing copper sensitivity in Cd-tolerant transgenics and Cd-sensitive mutants of Arabidopsis. Cadmium-tolerant transgenic plants that over-expressed A. thaliana phytochelatin synthase 1 (AtPCS1) were not tolerant of copper stress, thereby supporting the hypothesis that PC is not primarily involved in this tolerance mechanism. We also investigated Cu tolerance in cad2-1, a Cd-sensitive and glutathione (GSH)-deficient Arabidopsis mutant. Paradoxically, cad2-1 was more resistant to copper stress than were wild-type plants. This was likely due to the high level of cysteine present in that mutant. However, when the growth medium was supplemented with cysteine, the wild types also exhibited copper tolerance. Moreover, Saccharomyces cerevisiae that expressed AlPCSI showed tolerance to Cd but hypersensitivity to Cu. All these results indicate that PC is not a major factor in determining copper tolerance in plants.

참고문헌 (32)

  1. Clemens S, Kim EJ, Neumann D, Schroeder JI (1999) Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast. EMBO J 18: 3325-3333 
  2. de Miranda JR, Thomas MA, Thurman OA, Tomsett AB (1990) Metallothionein genes from the flowering plant Mimulus guttatus. FEBS Lett 260: 277-280 
  3. Ha S-B, Smith AP, Howden R, Dietrich WM, Bugg S, OConell MJ, Goldsbrough PB, Cobbett CS (1999) Phytochelatin synthase genes from Arabidopsis and the yeast, Schizosaccharomyces pombe. Plant Cell 11 : 1153-1164 
  4. Howden R, Cobbett CS (1992) Cadmium-sensitive mutants of Arabidopsis thaliana. Plant Physiol 99: 100-107 
  5. Lee S, Korban SS (2002) Transcriptional regulation of Arabidopsis thaliana phytochelatin synthase (AtPCS0) by cadmium during early stages of plant development. Planta 215: 689-693 
  6. Rauser WE (1999) Structure and function of metal chelators produced by plants: The case for organic acids, amino acids, phytin and metallothioneins. Cell Biochem Biophysics 32: 19-48 
  7. Vatamaniuk OK, Mari S, Lu Y-P, Rea PA (2000) Mechanism of heavy metal ion activation of phytochelatin (PC) synthase. J Biol Chem 275: 31451-31459 
  8. Cobbett CS, May MJ, Howden R, Rolls B (1998) The glutathione-deficient, cadmium-sensitive mutant, cad2-7, of Arabidopsis thaliana is deficient in $\gamma$-glutamylcysteine synthetase. Plant J 16: 73-78 
  9. van Vliet C, Anderson CR, Cobbett CS (1995) Copper-sensitive mutant of Arabidopsis thaliana. Plant Physioll 09: 871-878 
  10. de Vos CHRD, Vonk MJ, Vooijs R, Schat H (1992) Glutathione depletion due to copper induced phytochelatin synthesis causes oxidative stress in Silene cucubalus. Plant Physiol 98: 853-858 
  11. Grill E, Loffler 5, Winnacker E-L, Zenk MH (1989) Phytochelatins, the heavy-metal-binding peptides of plants, are synthesized from glutathione by a specific $\gamma$-glutamylcysteine dipeptidyl trans peptidase (phytochelatin synthase). Proc Natl Acad Sci USA 86: 6838-6842 
  12. Lee S, Petro D, Moon JS. Ko T-S, Goldsbrough PS, Korban SS (2003b) Higher levels of ectopic expression of Arabidopsis phytochelatin synthase do not lead to increased cadmium tolerance and accumulation. Plant Physiol Biochem 41: 903-910 
  13. Murashige T, Skoog T (1962) A revised medium for growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-479 
  14. Rauser WE (1990) Phytochelatins. Annu Rev Biochem 59: 61-86 
  15. Lee S, Moon JS, Ko T-S, Petro D, Goldsbrough PB, Korban SS (2003a) Overexpression of Arabidopsis phytochelatin synthase paradoxically leads to hypersensitivity to cadmium stress. Plant Physiol 131: 656-663 
  16. Murphy A, Taiz L (1995) Comparison of metallothionein gene expression and non-protein thiols in ten Arabidopsis ecotypes. Plant Physiol 109: 945-954 
  17. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, Ed 2. Cold Spring Harbor Laboratory Press, NY 
  18. Cobbett CS, Goldsbrough P (2002) Phytochelatins and metallothioneins: Roles in heavy metal detoxification and homeostasis. Annu Rev Plant Biol 53: 159-182 
  19. Lee S, Moon JS, Domier LL, Korban SS (2002) Molecular characterization of phytochelatin synthase expression in transgenic Arabidopsis. Plant Physiol Biochem 40: 727-733 
  20. Howden R, Goldsbrough PS, Anderson CR, Cobbett CS (1995) Cadmium-sensitive, cad7 mutants of Arabidopsis thaliana are phytochelatin deficient. Plant Physiol 107: 1059-1066 
  21. Zenk MH (1996) Heavy metal detoxification in higher plants: A review. Gene 179: 21-30 
  22. Grill E, Winnacker EL, Zenk MH (1987) Phytochelatins, a class of heavy-metal-binding peptides from plants, are functionally analogous to metallothioneins. Proc Natl Acad Sci USA 84: 439-443 
  23. Cobbett CS (2000b) Phytochelatin biosynthesis and function in heavy-metal detoxification. Curr Opin Plant Biol 3:211-216 
  24. Cobbett CS (2000a) Phytochelatins and their roles in heavy metal detoxification. Plant Physiol 123: 825-832 
  25. Hartley-Whitaker J, Ainsworth G, Meharg M (2001) Copper- and arsenate-induced oxidative stress in Holcus lanatus L. clones with differential sensitivity. Plant Cell Environ 24: 713-722 
  26. Scarano G, Morelli E (1998) Polarographic behavior of metal phytochelatin complexes. Electroanalysis 10: 3943 
  27. Vatamaniuk OK, Mari S, Lu Y-P Rea PA (1999) AtPCS1, a phytochelatin synthase from Arabidopsis: Isolation and in vitro reconstitution. Proc Natl Acad Sci USA 96: 7110-7115 
  28. Clemens S (2001) Molecular mechanisms of plant metal tolerance and homeostasis. Planta 212: 475-486 
  29. Oven M, Page JE, Zenk MH, Kutchan TM (2002) Molecular characterization of the homo phytochelatin synthase of soybean Glycine max. J Biol Chem 277: 4747-4754 
  30. Sandrine S-M, Stephan C, Patrick C, Catherine L-P, DoanTrung L, Gilles P (2003) Enhanced toxic metal accumulation in engineered bacterial cells expressing Arabidopsis thaliana phytochelatin synthase. Appl Environ Microbiol 69: 490-494 
  31. van Hoof NALM, Hassinen VH, Hakvoort HWJ, Ballintijn KF, Schat H, Verkleij JAC, Ernst WHOE, Karenlamp; SO, Tervanhauta AI (2001) Enhanced copper tolerance in Silene vulgaris (Moench) garcke populations from copper mine is associated with increased transcript levels of a 2b-type metallothionein gene. Plant Physiol 126: 1519-1526 
  32. Grill E, Winnacker EL, Zenk MH (1985) Phytochelatins: The principal heavy-metal complexing peptides of higher plants. Science 230: 674-676 

이 논문을 인용한 문헌 (7)

  1. 2007. "" Journal of plant biology = 식물학회지, 50(2): 220~223 
  2. 2008. "" Journal of plant biology = 식물학회지, 51(3): 192~201 
  3. Lee, Sang-Man 2009. "Heterologous Expression of Fission Yeast Heavy Metal Transporter, SpHMT-1, Confer Tolerance to Cadmium in Cytosolic Phytochelatin-Deficient Saccharomyces cerevisiae" 생명과학회지 = Journal of life science, 19(12): 1685~1689 
  4. Yoon, Ha-Im ; Kim, Jang-Eok ; Shin, Jae-Ho ; Kim, Jeong-Hoe ; Lee, Sang-Man 2009. "Effect of Phytochelatin Synthase Expression on Degradation of Fungicide Tolclofos-methyl in Mutant Plant and Transformed yeast" 한국환경농학회지 = Korean journal of environmental agriculture, 28(4): 409~411 
  5. 2010. "" Journal of the Korean Society for Applied Biological Chemistry, 53(5): 647~651 
  6. 2010. "" Journal of the Korean Society for Applied Biological Chemistry, 53(1): 94~96 
  7. 2011. "" Journal of the Korean Society for Applied Biological Chemistry, 54(5): 802~805 


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