[논문]Differential Functional Expression of Clotrimazole-sensitive <tex>$Ca^{2+}$</tex>-activated <tex>$K^+$</tex> Current in Bal-17 and WEHI-231 Murine B Lymphocytes

Zheng, Haifeng; Ko, Jae-Hong; Nam, Joo-Hyun; Earm, Yung-E; Kim, Sung-Joon

Differential Functional Expression of Clotrimazole-sensitive $Ca^{2+}$-activated $K^+$ Current in Bal-17 and WEHI-231 Murine B Lymphocytes 원문보기

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, v.10 no.1, 2006년, pp.19 - 24

Zheng, Haifeng (Department of Physiology and Biophysics, Seoul National University College of Medicine) , Ko, Jae-Hong (Department of Physiology and Biophysics, Seoul National University College of Medicine) , Nam, Joo-Hyun (Department of Physiology and Biophysics, Seoul National University College of Medicine) , Earm, Yung-E (Department of Physiology and Biophysics, Seoul National University College of Medicine) , Kim, Sung-Joon (Department of Physiology and Biophysics, Seoul National University College of Medicine)

Abstract ▼ AI-Helper

The intermediate conductance $Ca^{2+}-activated$ $K^+$ channels (SK4, IKCa1) are present in lymphocytes, and their membrane expression is upregulated by various immunological stimuli. In this study, the activity of SK4 was compared between Bal-17 and WEHI-231 cell lines which represent mature and immature stages of murine B lymphocytes, respectively. The whole-cell patch clamp with high-$Ca^{2+}$ ($0.8{\mu}M$) KCl pipette solution revealed a voltage-independent $K^+$ current that was blocked by clotrimazole (1 mM), an SK4 blocker. The expression of mRNAs for SK4 was confirmed in both Bal-17 and WEHI-231 cells. The density of clotrimazole-sensitive SK4 current was significantly larger in Bal-17 than WEHI-231 cells ($-11.4{\pm}3.1$ Vs. $-5.7{\pm}1.15$ pA/pF). Also, the chronic stimulation of B cell receptors (BCR) by BCR-ligation (anti-IgM Ab, $3{\mu}g$/ml, 8∼12 h) significantly upregulated the amplitude of clotrimazolesensitive current from $-11.4{\pm}3.1$ to $-53.1{\pm}8.6$ pA/pF in Bal-17 cells. In WEHI-231 cells, the effect of BCR-ligation was significantly small ($-5.7{\pm}1.15$ to $-9.0{\pm}1.00$ pA/pF). The differential expression and regulation by BCR-ligation might reflect functional changes in the maturation of B lymphocytes.

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RNAs were extracted from Bal-17 and WEHI-231 cells by guanidinium acid-phenol-chloroform method. RNA quanti fication was assessed at 260 nm.

참고문헌 (13)

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