Pre-Sowing Seed Treatment: Effect of Deep Sea Water Priming on Germination of Wild Vegetables; Gondalbi (Cirsium setidens), Jandae (Adenophora triphylla var. Joponica Hara) and Deoduck (Codonopsis lanceolata Trautv.)원문보기
This experiment was conducted on wild vegetables; Gondalbi (Cirsium setidens), Deoduck (Codonopsis lanceolata Trautv.), and Jandae (Adenophora triphylla var. Joponica Hara) seed to study whether priming with deep sea water results in enhancement of seed germination and identify the optimum concentra...
This experiment was conducted on wild vegetables; Gondalbi (Cirsium setidens), Deoduck (Codonopsis lanceolata Trautv.), and Jandae (Adenophora triphylla var. Joponica Hara) seed to study whether priming with deep sea water results in enhancement of seed germination and identify the optimum concentration of the priming solution, and duration of priming. Seeds were primed with 5 various concentrations (5%, 10%, 15%, 20% and 30%) of deep sea water (DSW) in 12 hours, 24 hours, and 48 hours at $24^{\circ}C$. Since Jandae had seed dormancy, it was kept for four weeks in refrigerator at $2^{\circ}C$ after priming treatment. In Deoduck, 5 percentage DSW priming significantly improved the early germination percentage, radicle length, and plumule emergence percentage. Among the priming period of treatments, 24 hours priming showed better performance in this treatment whereas, in Jandae, 12 hours priming with 10 percentages DSW significantly improved the germination percentage and germination rate. This treatment had increased the final germination percentage by 54%, 15% and 40% compared with control, plain water and $KNO_3$ priming respectively. But in Gondalbi, priming did not improve the germination of seed. However, among the priming treatments, 12 hours priming with 3% $KNO_3$ and 20% DSW gave better performance. In both the wild vegetables; Deoduck and Jandae, priming in deep sea water had improved the germination percentage and germination rate as compare to plain water, $KNO_3$, and without priming treatment. Hence the best seed priming treatment on Deoduck and Jandae are 24 hours with 5% DSW and 12 hours with 10% DSW respectively.
This experiment was conducted on wild vegetables; Gondalbi (Cirsium setidens), Deoduck (Codonopsis lanceolata Trautv.), and Jandae (Adenophora triphylla var. Joponica Hara) seed to study whether priming with deep sea water results in enhancement of seed germination and identify the optimum concentration of the priming solution, and duration of priming. Seeds were primed with 5 various concentrations (5%, 10%, 15%, 20% and 30%) of deep sea water (DSW) in 12 hours, 24 hours, and 48 hours at $24^{\circ}C$. Since Jandae had seed dormancy, it was kept for four weeks in refrigerator at $2^{\circ}C$ after priming treatment. In Deoduck, 5 percentage DSW priming significantly improved the early germination percentage, radicle length, and plumule emergence percentage. Among the priming period of treatments, 24 hours priming showed better performance in this treatment whereas, in Jandae, 12 hours priming with 10 percentages DSW significantly improved the germination percentage and germination rate. This treatment had increased the final germination percentage by 54%, 15% and 40% compared with control, plain water and $KNO_3$ priming respectively. But in Gondalbi, priming did not improve the germination of seed. However, among the priming treatments, 12 hours priming with 3% $KNO_3$ and 20% DSW gave better performance. In both the wild vegetables; Deoduck and Jandae, priming in deep sea water had improved the germination percentage and germination rate as compare to plain water, $KNO_3$, and without priming treatment. Hence the best seed priming treatment on Deoduck and Jandae are 24 hours with 5% DSW and 12 hours with 10% DSW respectively.
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문제 정의
The objective of this experiment was to study whether priming with deep sea water results in enhancement of seed germination and identi fy the suitable concentration and period of priming. The optimization of seed priming techniques becomes very important to seed priming.
제안 방법
30 seeds were used in each treatment. In addition to plain water and 3% KNCh, required concentrations of the Deep Sea \Vhter (5%, 10%, 15% 20% and 30%) were prepared and seeds were folly immersed in pri ming media at a temperature of 25 °C for duration of 12, 24 and 48 hours after the first batch, respectively, so that all the seed was removed from media at the same time. After the priming, seeds were rinsed with dis tilled water.
대상 데이터
30 seeds were used in each treatment. In addition to plain water and 3% KNCh, required concentrations of the Deep Sea \Vhter (5%, 10%, 15% 20% and 30%) were prepared and seeds were folly immersed in pri ming media at a temperature of 25 °C for duration of 12, 24 and 48 hours after the first batch, respectively, so that all the seed was removed from media at the same time.
of Kangwon National University in 2006. Gondalbi seeds (Cirsium setidens), Deoduck (Codonopsis lanceolata Trautv.) and Jandae (Adenophora triphylla var. Joponica Hara) was used in the experiment. Following pre-sowing seed treatments were included.
데이터처리
The data collected was analyzed using the Fisher's analy sis of variance technique under completely randomized de sign (CRD) with MSTAT software program and the treat ment means were compared by Least Significant Difference (LSD) test at 0.05 probability level (Steel and Torri, 1984).
이론/모형
Ten seeds per petridish were sown and the experi ment was replicated thrice. This experiment was laid out in fac torial Con^letely Randomized Design (CRD). Germination of seeds was evaluated on 55 millimeter diameter Whatman no.
성능/효과
The 5% DSW gave significantly highest percentage of germi nation, radicle length (24mM) and plumule percentage (57%) in 24 hours priming. 53% of germination was counted in early days (5DAS) where as it was only 7%, 33% and 30% in KNO3, plain water priming and without priming respectively. As far as the final germina tion percentage is concerned, highest germination (87%) was ob tained in this treatment whereas it was 43%, 77% and 67% in KNO3, plain water priming and without priming respectively.
2) was recorded in this treatment (Table 3). As far as the final germination percentage is concerned, this 10% DSW treatment also gave the highest germination (87%) whereas KNO3, plain water and without priming had 47%, 73% and 33%respectively. Thisresult supportsthe finding ofFly and Heydecker (1981) who had found that priming improved the germination ofParsley seed.
Among the three duration ofpriming, 12 hours ofpriming had significantly enhanced the germination percentage and germination rate. Highest germination percentage; 48%, 59%, 62% and 62% wasrecorded on 5DAS, 7DAS, 9DAS and 11 DAS, respectively whereas it was only 34%, 44%, 50% and 51 % in 24 hours priming, and 37%, 46%, 52%, and 52% in 48 hours priming on above mentioned days respectively. Similarly, the significantly highest gemination rate (3.
2). The 5% DSW gave significantly highest percentage of germi nation, radicle length (24mM) and plumule percentage (57%) in 24 hours priming. 53% of germination was counted in early days (5DAS) where as it was only 7%, 33% and 30% in KNO3, plain water priming and without priming respectively.
The effect ofprimingtreatment was highly significant on germina-tion percentage and germination rate. The highest percentage ofgermination on all the recorded days was noticed in 15% DSW treatment followed by 3% KNO& Hence, deep sea water priming improved the germination percentage and rate (Fig. 3).
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