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제안 방법
- Identification of three isolates was examined by sequencing their partial ITS regions. The assay was accomplished by amplifying two ITS regions between 18S and 28S and one ITS region in 5.
- In conclusion, this study is based on the fundamental observation on the dollar spot and fungal pathogens. Unfortunately, in this study, use of limited sample numbers collected does not seem to provide a full understanding of the relationship between genetic diversity of pathogen population and their characteristics such as virulence and fungicide sensitivity.
- BLAST search through ITS sequencing is currently a common method for fungal or bacterial identification(Clarridge, 2004). In this experiment, ITS sequencing was accomplished by using two sequencing primers, which were constructed from two ITS regions between 18S and 28S, and also from one ITS region in 5.8S region(White et al., 1990). ITS regions from three isolates were investigated by the partial sequencing rather than the entire sequencing.
- Identification of three isolates was examined by sequencing their partial ITS regions. The assay was accomplished by amplifying two ITS regions between 18S and 28S and one ITS region in 5.8S region using PCR(White et al, 1990). For the sequences of ITS regions from three isolates, primers used were as f이 low; rrSl(5'-TCCGTAGGTGAACCTGCGG-3'), ITS4(5'-TCCTCCGCTTATTG ATATGC-3'), ITS2(5'-GCTGCGTTCTTCATCGATGC-3'), ITS3(5'-GCATCGATGAAGAA CGCAGC3).
- The assay was conducted to determine the sensitivity degree of three isolates, against propiconazole, a representative fungicide for dollar spot. Mycelia of Scbl, Sczl, and Scz2 were incubated for 24 and 48 hours on PDA plates containing various concentrations of propiconazole; 0.
- The objective of this study was to confirm a new collected isolate as S. homoeocarpa using molecular techniques, to investigate the genetic discrepancies between two isolates, Sczl and Scbl, revealing different host specificities, and to test the degree of sensitivity of two analogous strains, Sczl and Scz2, through the fungicide bioassay.
대상 데이터
- , USA). Both identification and alignment of the partial sequences were assessed by using NCBI BLAST(Basic Local Alignment Search Tool) through the intemet(httpe7www.ncbi.nlm.nih.goY/BLAS, I/). As a reference, 18S rDNA sequence of S.
- Infected stems and leaves of zoysiagrass were harvested at fairways of golf courses in the Gyeonggi province of Korea. The infected tissues cut into 5 mm length were surface-sterilized with 70 % ethanol for 1 minute twice then placed on the potato dextrose agar(PDA) medium at 25℃.
- 4 ㎕, for a total of 20 ㎕ reaction mixture. Twenty random primers(OPERON Technologies Inc., U.S.A.) were used for the analysis. Amplification reactions were carried out on the GeneAmp PCR System 2400(Perkin-Elmer) programmed for 5 min at 95 ℃ followed by 40 cycles of 1 min at 95 ℃ (denaturation), 1 min at 37 ℃ (annealing) and 2 min at 72 ℃ (extension) and a final stage of 10 min at 72 ℃.
데이터처리
- (1983) used. The data obtained from three repeated experiments were determined by one-way ANOVA test.
이론/모형
- 2). Based on the statistical analysis of data scoring bands on the gels scored as '1' to the presence and 'O' to the absence of a band, the unweighted pair-group method using arithmetic average (UPGMA) was used to construct the genetic similarity matrix(Table 2). Similarity matrix analysis using Jaccardes similarity showed that the datum was almost consistent with the result from ITS analysis.
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