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AI 본문요약
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문제 정의

  • 본 고찰에서는 본 연구실에서 개발한 인간 유래 지지세포를 이용한 인간 배아줄기세포의 배양에 관한 연구결과를 포함하여, 최근까지 수행된 다양한 인간 지지세포를 이용한 배양, 지지세포를 배제한 조건배양액 (conditioned media; CM)을 이용한 배양 및 성분이 알려진 배양액 개발 등 인간 배아줄기세포의 배양 조건의 개선을 위한 연구들에 관하여 살펴보고자 한다.
  • 이상에서와 같이 인간 배아줄기세포의 확립과 배양에 있어 사용된 다양한 지지세포 및 배양액에 관해 살펴보았다. 또한, 본 연구실에서 연구된 양수 세포를 지지세포로 이용한 인간 배아줄기세포 배양 결과에 관해 살펴보고, 그 밖의 다양한 인간 유래 지지세포, 지지세포를 배제한 배양 (feeder-free culture) 및 성분이 알려진 배양액 (defined media) 에관하여 살펴보았다.
본문요약 정보가 도움이 되었나요?

참고문헌 (58)

  1. Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, et al. Embryonic stem cell lines derived from human blastocysts. Science 1998; 282: 1145-7 

  2. Tosh D, Slack JM. How cells change their phenotype. Nat Rev Mol Cell Biol 2002; 3: 187-94 

  3. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defmed factors. Cell 2006; 126: 1-14 

  4. De Coppi P, Bartsch G Jr, Siddiqui MM, Xu T, Santos CC, Perin L, et al. Isolation of anmiotic stem cell lines with potential for therapy. Nat Biotechnol 2007; 25: 100-6 

  5. Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat Biotechnol 2000; 18: 399-404 

  6. Oh SK, Kim HS, Ahn HJ, Seol HW, Kim YY, Park YB, et al. Derivation and characterization of new human embryonic stem cell lines, SNUhES1, SNUhES2, and SNUhES3. Stem Cells 2005; 23: 211-9 

  7. Assady S, Maor G, Amit M, Itskovitz-Eldor J, Skorecki K, Tzukerman M. Insulin production by human embryonic stem cells. Diabetes 2001; 50: 1691-7 

  8. Carpenter MK, Inokuma MS, Denham J, Mujtaba T, Chiu CP, Rao MS. Enrichment of neurons and neural precursors from human embryonic stem cells. Exp Neurol 2001; 172: 383-97 

  9. Kehat I, Kenyagin-Karsenti D, Snir M, Segev H, Amit M, Gepstein A, et al. Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes. J Clin Invest 2001; 108: 407-14 

  10. Amit M, Margulets V, Segev H, Shariki K, Laevsky I, Coleman R, et al. Human feeder layers for human embryonic stem cells. Biol Reprod 2003; 68: 2150-6 

  11. Laslett AL, Filipczyk AA, Pera ME Characterization and culture of human embryonic stem cells. Trends Cardiovasc Med 2003; 13: 295-301 

  12. Prowse AB, McQuade LR, Bryant KJ, Marcal H, Gray PP. Identification of potential pluripotency determinants for human embryonic stem cells following proteomic analysis of human and mouse fibroblast conditioned media. J Proteome Res 2007; 10.1021/pr0702262 

  13. Kawano Y, Kypta R. Secreted antagonists of the Wnt signaling pathway. J Cell Sci 2003; 116: 2627-34 

  14. Sato N, Meijer L, Skaltsounis L, Greengard P, Brivanlou AH. Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor. Nat Med 2004; 10: 55-63 

  15. Canalis E, Economides AN, Gazzerro E. Bone morphogenetic proteins, their antagonists, and the skeleton. Endocr Rev 2003; 24: 218-35 

  16. Beattie GM, Lopez AD, Bucay N, Hinton A, Firpo MT, King CC, et al. Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers. Stem Cells 2005; 23: 489-95 

  17. Evans MJ, Kaufinan MH. Establishment in culture of pluripotential cells from mouse embryos. Nature 1981; 292: 154 -6 

  18. Williams R, Hilton D, Pease S, Wilson T, Stewart C, Gearing D, et al. Myeloid leukemia inhibitory factor maintains the developmental potential of embryonic stem cells. Nature 1988; 336: 684-7 

  19. Lim KWE, Bodnar A. Proteome analysis of conditioned medium from mouse embryonic fibroblast feeder layers which support the growth of human embryonic stem cell. Proteomics 2002; 2: 1197-203 

  20. Martin MJ, Muotri A, Gage F, Varki A. Human embryonic stem cells express an immunogenic nonhuman sialic acid. Nat Med 2005; 11: 1-5 

  21. 김희선, 설혜원, 안희진, 오선경, 구승엽, 김석현 등. 양수세포를 이용한 인간 배아줄기세포의 배양. 대한불임학회지 2004; 31: 261-71 

  22. Prusa AR, Hengstschlager M. Amniotic fluid cells and human stem cell research: a new connection. Med Sci Monit 2002; 8(11):RA253-7 

  23. Prusa AR, Marton E, Rosner M, Bemaschek G, Hengstschlager M. Oct-4-expressing cells in human amniotic fluid: a new source for stem cell research? Hum Reprod 2003; 18(7): 1489 3-93 

  24. Richards M, Fong CY, Chan WK, Wong PC, Bongso A. Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat Biotechnol 2002; 20: 933-6 

  25. Richards M, Tan S, Fong CY, Biswas A, Chan WK., Bongso A. Comparative evaluation of various human feeders for pro- 3 longed undifferentiated growth of human embryonic stem cells. Stem Cells 2003; 21: 546-56 

  26. Hovatta O, Mikkola M, Gertow K, Stromberg AM, Inzunza J, Hreinsson J, et al. A culture system using human foreskin 3 fibroblasts as feeder cells allows production of human embryonic stem cells. Hum Reprod 2003; 18: 1404-9 

  27. Amit M, Shariki C, Margulets V, Itskovitz-Eldor J. Feeder layer and serum-free culture of human embryonic stem cells. Biol Reprod 2004; 70: 837-45 

  28. Cheng L, Hammond H, Ye Z, Zhan X, David G. Human adult marrow cells support prolonged expansion of human embryonicstem cells in culture. Stem Cells 2003; 21: 131-42 

  29. Genbacev O, Krtolica A, Zdravkovic T, Brunette E, Powell S, Nath A, et al. Serum-free derivation of human embryonic stem cell lines on human placental fibroblast feeders. Fertil Steril 2005; 83: 1517-29 

  30. Kim SJ, Song CH, Sung HJ, Yoo YD, Geum DH, Park SH, et al. Human placenta-derived feeders support prolonged undifferentiated propagation of a human embryonic stem cell line, SNUhES3: comparison with human bone marrowderived feeders. Stem Cells Dev 2007; 16: 421-8 

  31. Xu C, Jiang J, Sottile V, McWhir J, Lebkowski J, Carpenter MK. Immortalized fibroblast-like cells derived from human embryonic stem cells support undifferentiated cell growth. Stem Cells 2004; 22: 972-80 

  32. Stojkovic P, Lako M, Stewart R, Przyborski S, Armstrong L, Evans J, et al. An autogeneic feeder cell system that efficiently supports growth of undifferentiated human embryonic stem cells. Stem Cells 2005; 23: 306-14 

  33. Wang Q, Fang ZF, Jin F, Lu Y, Gai H, Sheng HZ. Derivation and growing human embryonic stem cells on feeders derived from themselves. Stem Cells 2005; 23: 1221-7 

  34. Xu C, Inokuma MS, Denham J, Golds K, Kundu P, Gold ill, et al. Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol 2001; 19: 971-4 

  35. Carpenter MK, Rosler ES, Fisk GJ, Brandenberger R, Ares X, Miura T, et a1. Properties of four human embryonic stem cell lines maintained in a feeder-free culture system. Dev Dyn 2004; 229: 243-58 

  36. Noaksson K, Zoric N, Zeng X, Rao MS, Hyllner J, Semb H, et al. Monitoring differentiation of human embryonic stem cells using real-time PCR. Stem Cells 2005; 23: 1460-7 

  37. Wang G, Zhang H, Zhao Y, Li J, Cai J, Wang P, et al. Noggin and bFGF cooperate to maintain the pluripotency of human embryonic stem cells in the absence of feeder layers. Biochem Biophys Res Commun 2005; 330: 934-42 

  38. Ellerstrom C, Strehl R, Moya K, Andersson K, Bergh C, Lundin K, et al. Derivation of axe no-free human embryonic stem cell line. Stem Cells 2006; 24: 2170-6 

  39. Rajala K, Hakala H, Panula S, Avio S, Pihlajamaki H, Suuronen R, et al. Testing of nine different xeno-free culture media for human embryonic stem cell cultures. Hum Reprod 2007; 22: 1231-8 

  40. Amit M, Carpenter MK, Inokuma MS, Chiu CP, Harris CP, Waknitz MA, et al. Clonally derived human embryonic stem cell lines maintain plilripotency and proliferative potential for prolonged periods of culture. Dev Biol 2000; 227: 271-8 

  41. Koivisto H, Hyvarinen M, Stromberg AM, Inzunza J, Matilainen E, Mikkola M, et al. Cultures of human embryonic stem cells: serum replacement medium or serum-containing media and the effect of basic fibroblast growth factor. Reprod Biomed Online 2004; 9: 330-7 

  42. Li Y, Powell S, Brunette E, Lebkowski J, Mandalam R. Expansion of human embryonic stem cells in defmed serumfree medium devoid of animal-derived products. Biotechnol Bioeng 2005; 91: 688-98 

  43. Vallier L, Alexander M, Pedersen RA. Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells. J Cell Sci 2005; 118: 4495-509 

  44. Ludwig T, Levenstein M, Jones J, Berggren W, Mitchen E, Frane JL, et al. Derivation of human embryonic stem cells in defined conditions. Nat Biotechnol 2006; 24: 185-7 

  45. Ludwig TE, Bergendahl V, Levenstein ME, Yu J, Probasco MD, Thomson JA. Feeder-independent culture of human embryonic stem cells. Nat Met 2006; 3: 637-46 

  46. Klimanskaya I, Chung Y, Meisner L, Johnson J, West M, Lanza R. Human embryonic stem cells derived without feeder cells. Lancet 2005; 365: 1636-41 

  47. Stojkovic P, Lako M, Przyborski S, Stewart R, Armstrong L, Evans J, et al. Human-serum matrix supports undifferentiated growth of human embryonic stem cells. Stem Cells 2005; 23: 895-902 

  48. Lanzendorf SE, Boyd CA, Wright DL, Muasher S, Oehninger S, Hodgen GD. Use of human gametes obtained from anonymous donors for the production of human embryonic stem cell lines. Fertil Steril 2001; 76: 132-7 

  49. Amit M, Itskovitz-Eldor J. Differentiation and spontaneous differentiation of human embryonic stem cells. J Anat 2002; 200: 225-32 

  50. Mitalipova M, Calhoun J, Shin S, Wininger D, Schulz T, Noggle S, et al. Human embryonic stem cell lines derived from discarded embryos. Stem Cells 2003; 21: 521-6 

  51. Heins N, Englund MC, Sjoblom C, Dahl U, Tonning A, Bergh C, et al. Derivation, characterization, and differentiation of human embryonic stem cells. Stem Cells 2004; 22: 367-76. 

  52. Suss-Toby E, Gerecht-Nir S, Amit M, Manor D, ItskovitzEldor J. Derivation of a diploid human embryonic stem cell line from a mononuclear zygote. Hum Reprod 2004; 19: 670-5 

  53. Cowan CA, Klimanskaya I, McMahon J, Atienza J, Witmyer J, Zucker JP, et al. Derivation of embryonic stem-cell lines from blastocysts. N Engl J Med 2004; 350: 1353-6 

  54. Baharvand H, Ashtiani SK, Valojerdi MR, Shahverdi A, Taee A, Sabour D. Establishment and in vitro differentiation of a new embryonic stem cell line from human blastocyst. Differentiation 2004; 72: 224-9 

  55. Stojkovic M, Lako M, Stojkovic P, Stewart R, Przyborski S, Armstrong L, et al. Derivation of human embryonic stem cells from day-8 blastocysts recovered after three-step in vitro culture. Stem Cells 2004; 22: 790-7 

  56. Strelchenko N, Verlinsky O, Kukharenko V, Verlinsky Y. Morula-derived human embryonic stem cells. Repro Biomed Online 2004; 9: 623-9 

  57. Li T, Zhou CQ, Mai QY, Zhuang GL. Establishment of human embryonic stem cell line from gamete donors. Chin Med J (Engl) 2005; 118: 116-22 

  58. Inzunza J, Gertow K, Stromberg MA, Matilainen E, Blennow E, Skottman H, et al. Derivation of human embryonic stem cell lines in serum replacement medium using postnatal human fibroblasts as feeder cells. Stem Cells 2005; 23: 544-9. 

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