[논문]Expression of Cytokines in Radiation Injured Brain at Acute Phase

Lee, Jang-Bo; Kim, Min-Ho; Chung, Yong-Gu; Park, Jung-Yul

Expression of Cytokines in Radiation Injured Brain at Acute Phase 원문보기

Journal of Korean Neurosurgical Society = 대한신경외과학회지, v.42 no.3, 2007년, pp.200 - 204

Lee, Jang-Bo (Department of Neurosurgery College of Medicine, Korea University) , Kim, Min-Ho (Department of Neurosurgery College of Medicine, Korea University) , Chung, Yong-Gu (Department of Neurosurgery College of Medicine, Korea University) , Park, Jung-Yul (Department of Neurosurgery College of Medicine, Korea University)

Abstract ▼ AI-Helper

Objective : Radiation therapy is an important treatment for brain tumor. However, serious complications such as radiation necrosis can occur and it may be secondary to the expression of acute phase genes, like cytokines. In particular, inflammatory cytokines (IL-$1{\beta}$, TNF-${\alpha}$) and other immunomodulatory cytokines (TNF-${\alpha}$, TGF-${\beta}1$) might be changed after irradiation (high single dose irradiation). Although it has been reported that IL-1 level is remarkably elevated within 8 week after the irradiation to the rat brain. the change of cytokines levels at acute phase (within 24 hours) has not been reported. In the present study, we examined TNF-${\alpha}$, TGF-${\beta}1$, and IL-$1{\beta}$ levels in acute phase to clarify the early effect of cytokines on the radiation-induced brain damage. Methods : Fifty Sprague-Dawley rats were used and these were divided into irradiation group and control group. After a burr-hole trephination on the right parietal area using a drill, a single 10Gy was irradiated at the trephined site. Their forebrains were extirpated at 30 min, 2 hr, 8 hr, 12 hr and 24 hr, respectively and examined for the expression of TNF-${\alpha}$, TGF-${\beta}1$, and IL-$1{\beta}$. Results : The expression of TNF-${\alpha}$ and TGF-${\beta}1$ were decreased until 12 hr after irradiation but elevated thereafter. The expression of IL-1 was peak at 8 hr and then decreased until 12 hr but elevated after this time window. The present study indicated that expression of cytokines (TNF-${\alpha}$, TGF-${\beta}1$ and IL-$1{\beta}$) were increased at 24 hr after the irradiation to the rat brain. IL-$1{\beta}$ level, on the other hand. reached peak at 8 hr after radiation injury. Conclusion : These findings indicate that IL-1, among various cytokines, may have a more important role in the inflammatory reaction by radiation injury at acute phase and provide some clues for better understanding of the pathogenesis of radiation injury.

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문제 정의

Therefore, if mechanism of cytokines in brain tissue after radiotherapy can be revealed, it may provide an important clue to prevent a complication through regulating expression of cytokines. Hence, the aim of this study was to elucidate the expression of various cytokines (e.g., IL-1炒 TNF-cr and TGF-J01) at acute phase in normal rat brain after irradiation.

제안 방법

In this study, the expression of cytokines (TNF-a, TGF- B' and IL-1 仞 was compared with that of dififerent periods by ELISA assay to evaluate the expression of cytokines of acute stage (30 min-24 hr) after irradiation in normal rat brain. The cytokine expression of radiation group was higher than those of the control group in almost times.
Twenty-five rats were divided into 5 groups, and their forebrains were extirpated at 30 min, 2 hour, 8 hour, 12 hour, and 24 hour after irradiation. In the control group, 25 non-irradiated rats also underwent forebrain extirpation at the same time intervals as the irradiation group.

대상 데이터

Fifty female Sprague-Dawley rats weighing 200-300 g were used. The rats were given water and food ad libitum until the experiment was conducted.

데이터처리

The expressions of IL-1/3, TNF-a and TGF-/J1 from each group were statistically analyzed using the Duncan method of the one-way ANOVA procedure of SPSS (2004). For each group, the mean and SD for IL-1/3, TNF-tz and TGFel were calculated.

성능/효과

20) reported that TNF-a injected rats before radiation showed higher survival rates than those without protection efifect of hematopoetic system. This study has also shown that TNF-a- expression in the irradiated rat brain was higher than those of the control group except for 8 hr and 12 hr(/><0.05). These results surest that TNF-a play an important r시e in the irradiated brain by regulating physiological radiation resistance and restoration.

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