[논문]Assessment on Antioxidant Properties of Oplopanax elatus Nakai in vitro

Kim, Jun-Heong; Eom, Seok-Hyun; Lee, Han-Shin; Kim, Jae-Kwang; Yu, Chang-Yeon; Kwon, Yong-Soo; Lee, Ju-Kyong; Kim, Myong-Jo

Assessment on Antioxidant Properties of Oplopanax elatus Nakai in vitro 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.15 no.2, 2007년, pp.112 - 119

Kim, Jun-Heong (Bioherb Research Institute, Kangwon National University) , Eom, Seok-Hyun (College of Bioscience & Biotechnology, Kangwon Natl. Univ.) , Lee, Han-Shin (College of Bioscience & Biotechnology, Kangwon Natl. Univ.) , Kim, Jae-Kwang (National Institute of Agricultural Biotechnology, RDA) , Yu, Chang-Yeon (Bioherb Research Institute, Kangwon National University) , Kwon, Yong-Soo (College of Phamacy, Kangwon National University) , Lee, Ju-Kyong (Bioherb Research Institute, Kangwon National University) , Kim, Myong-Jo (Bioherb Research Institute, Kangwon National University)

Abstract ▼ AI-Helper

Effects of Oplopanax elatus have known to various pharmaceutical therapies. However, chemical prosperities in the plant are rarely investigated. In order to detect biological activity, we evaluated the antioxidant activity of five fractions from methanolic extracts in each part of O. elatus. Also, contents of polyphenols and polysaccharides were measured. Five fractions were of sub-fractions using n-hexane, ethyl-acetate, n-butanol, and water from methanolic extracts. Ethylacetate and n-butanol fractions from stem and root exhibited strong antioxidant activity and high total phenolics content. On the HPLC analysis, ten free phenolics, including p-hydroxybenzoic acid, chlorogenic acid, caffeic acid, syringic acid, p-cou maric acid, ferulic acid, naringin, hesperidin, quercetin and trans-cinnamic acid, were identified from the fractions and were shown to different quantitative proportions. Furthermore, ethylacetate and n-butanol fraction had the highest amount of chlorogenic acids, one of the cinnamic acid derivatives possessing pharmacological properties. These results indicated that the fractions of O. elatus, as well as methanolic extracts, could be used as natural antioxidative ingredients.

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문제 정의

elatus and determine principal phenolic phytochemicals. This study may be helpful to understand pharmaceutical behaviour of the plant extract.

제안 방법

In this paper, we evaluate the possible antioxidant activity of extracts and solvent fractions from leae stem, root in O. elatus and determine principal phenolic phytochemicals. This study may be helpful to understand pharmaceutical behaviour of the plant extract.

대상 데이터

Chemicals used in this study were purchased from Sigma (Sigma Chemical Co., St. Louis, MO, USA), with the exception of reagent-grade solvents. Methanol, w-hexane, EtOAc, BuOH, and ethanol were purchased from Dongyang (Korea).
Louis, MO, USA), with the exception of reagent-grade solvents. Methanol, w-hexane, EtOAc, BuOH, and ethanol were purchased from Dongyang (Korea). All chemicals and reagents were of analytical grade.
, Korea). The HPLC system consists of a SP930D pump, a MIDAS auto-sampler, a UV730D ultraviolet-visible detector and a CTS30 column oven. Peak area was calculated using Yonglin autochro-3000 vl.
elatus were collected from Hwa-ak mountain, Gyeonggi province, Korea in April, 2004. The plants were identified by Dr. K. Heo (Department of Plant Science Program, Faculty of Bio-resource Engineering, Kangwon National University). Voucher specimens of these plants were deposited in The Herbarium of Kangwon Herbarium of Kangwon National University.
Heo (Department of Plant Science Program, Faculty of Bio-resource Engineering, Kangwon National University). Voucher specimens of these plants were deposited in The Herbarium of Kangwon Herbarium of Kangwon National University. Dried samples of O.

이론/모형

The FTC method reported by Inatani (Inatani et al., 1983) was used in this study. A mixture of 0.
After acidic hydrolysis, the liquidsolid mixture was diluted to 50 mE with distilled water. The supernatant was separated by sedimentation, and the polysaccharide in the supernatant assayed according to the carbazole reaction method of Zhang (Zhang et al., 1996).

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