목 적: 미성숙 난자의 체외성숙에 있어 난구세포의 양상 및 미성숙 난자의 크기가 영향 인자로 작용하는지를 알아보고자 하였다. 연구방법: 체외수정을 위한 과배란유도 후 난자채취를 시행한 21명의 환자로부터 41개의 난포 단계의 난자를 얻었다. Denudation 이전의 난구세포의 모양에 따라 dispersed 난구세포와 compacted 난구세포로 나누었다. Denudation 이후 투명대를 포함하는 난자외직경 (outer oocyte diameter)과 투명대를 포함하지 않는 난자내직경 (inner oocyte diameter)을 측정하고 체외성숙 배양액에서 체외성숙을 시도하였다. 난자의 성숙은 제1극체가 나온 경우로 정하였다. 성숙이 확인된 난자는 ICSI 방법을 이용하여 수정시키고 다음날 두 개의 전핵이 뚜렷이 보이는 경우에 수정된 것으로 간주하였다. 결 과: 전체적인 체외성숙률은 56.1%, 수정률은 73.9%이었다. Dispersed 난구세포 난자 중에서 성숙된 난자 비율 (91.3%)은 비성숙 난자 비율 (55.6%)보다 유의하게 높았다 (p=0.023). 성숙 또는 비성숙 난자에서 난자외경($155.7{\mu}m$ vs. $152.4{\mu}m$, NS)과 내경 ($114.3{\mu}m$ vs. $113.4{\mu}m$, NS) 및 투명대 두께 ($41.3{\mu}m$ vs. $39.1{\mu}m$, NS)는 차이가 없었다. 체외성숙률은 dispersed 난구세포 난자에서 compacted 난구세포 난자보다 의미있게 높았으나 (67.7% vs. 20.0%, p=0.044), 수정률에 있어 두 군간의 유의미한 차이는 없었다. 난자의 외경, 내경 및 투명대 두께에 따른 체외성숙률 및 수정률의 차이가 없었다. 결 론: Dispersed 난구세포를 가진 미성숙 난자가 compacted 난구세포를 가졌던 난자에 비하여 더 높은 체외수정능을 가진다는 결과는 dispersed 난구세포가 미성숙 난자의 체외성숙 예측을 위한 하나의 지표로 사용될 수 있음을 시사한다.
목 적: 미성숙 난자의 체외성숙에 있어 난구세포의 양상 및 미성숙 난자의 크기가 영향 인자로 작용하는지를 알아보고자 하였다. 연구방법: 체외수정을 위한 과배란유도 후 난자채취를 시행한 21명의 환자로부터 41개의 난포 단계의 난자를 얻었다. Denudation 이전의 난구세포의 모양에 따라 dispersed 난구세포와 compacted 난구세포로 나누었다. Denudation 이후 투명대를 포함하는 난자외직경 (outer oocyte diameter)과 투명대를 포함하지 않는 난자내직경 (inner oocyte diameter)을 측정하고 체외성숙 배양액에서 체외성숙을 시도하였다. 난자의 성숙은 제1극체가 나온 경우로 정하였다. 성숙이 확인된 난자는 ICSI 방법을 이용하여 수정시키고 다음날 두 개의 전핵이 뚜렷이 보이는 경우에 수정된 것으로 간주하였다. 결 과: 전체적인 체외성숙률은 56.1%, 수정률은 73.9%이었다. Dispersed 난구세포 난자 중에서 성숙된 난자 비율 (91.3%)은 비성숙 난자 비율 (55.6%)보다 유의하게 높았다 (p=0.023). 성숙 또는 비성숙 난자에서 난자외경($155.7{\mu}m$ vs. $152.4{\mu}m$, NS)과 내경 ($114.3{\mu}m$ vs. $113.4{\mu}m$, NS) 및 투명대 두께 ($41.3{\mu}m$ vs. $39.1{\mu}m$, NS)는 차이가 없었다. 체외성숙률은 dispersed 난구세포 난자에서 compacted 난구세포 난자보다 의미있게 높았으나 (67.7% vs. 20.0%, p=0.044), 수정률에 있어 두 군간의 유의미한 차이는 없었다. 난자의 외경, 내경 및 투명대 두께에 따른 체외성숙률 및 수정률의 차이가 없었다. 결 론: Dispersed 난구세포를 가진 미성숙 난자가 compacted 난구세포를 가졌던 난자에 비하여 더 높은 체외수정능을 가진다는 결과는 dispersed 난구세포가 미성숙 난자의 체외성숙 예측을 위한 하나의 지표로 사용될 수 있음을 시사한다.
Objective: This study was performed to investigate whether cumulus morphology and oocyte diameter influence on in vitro maturation (IVM) of human germinal vesicle (GV) stage oocytes obtained from stimulated in vitro fertilization (IVF) cycles. Methods: Forty-one GV stage oocytes were obtained from 2...
Objective: This study was performed to investigate whether cumulus morphology and oocyte diameter influence on in vitro maturation (IVM) of human germinal vesicle (GV) stage oocytes obtained from stimulated in vitro fertilization (IVF) cycles. Methods: Forty-one GV stage oocytes were obtained from 21 patients who received ovarian hyperstimulation and IVF. According to cumulus morphology before denudation, GV oocytes were classified into oocytes with dispersed cumulus cells (CCs) or compacted CCs. The diameters of denuded oocytes, both including and excluding the zona pellucida, were measured. All oocytes were cultured in commercial IVM medium. Maturation was defined as extrusion of the first polar body and the matured oocytes were inseminated by ICSI method. Results: Overall maturation and fertilization rate were 56.1% and 73.9%. Matured oocytes had significantly higher proportion of oocytes with dispersed CCs compared to oocytes failed to mature (91.3% vs. 55.6%, p=0.023). There were no significant differences of oocytes outer ($155.7{\mu}m$ vs. $152.4{\mu}m$, NS), inner ($114.3{\mu}m$ vs. $113.4{\mu}m$, NS) diameters and zona thicknesses ($41.3{\mu}m$ vs. $39.1{\mu}m$, NS) between matured and not-matured oocytes. In-vitro maturation rate of oocytes with dispersed CCs was significantly higher than which of oocytes with compacted CCs (67.7% vs. 20.0%, p=0.044). Oocyte diameters (outer and inner) and thicknesses were not related with maturational competence. Conclusion: Our results suggest that in vitro maturational competence of GV stage oocytes obtained from stimulated IVF cycles is closely associated with the cumulus morphology but not oocyte diameter.
Objective: This study was performed to investigate whether cumulus morphology and oocyte diameter influence on in vitro maturation (IVM) of human germinal vesicle (GV) stage oocytes obtained from stimulated in vitro fertilization (IVF) cycles. Methods: Forty-one GV stage oocytes were obtained from 21 patients who received ovarian hyperstimulation and IVF. According to cumulus morphology before denudation, GV oocytes were classified into oocytes with dispersed cumulus cells (CCs) or compacted CCs. The diameters of denuded oocytes, both including and excluding the zona pellucida, were measured. All oocytes were cultured in commercial IVM medium. Maturation was defined as extrusion of the first polar body and the matured oocytes were inseminated by ICSI method. Results: Overall maturation and fertilization rate were 56.1% and 73.9%. Matured oocytes had significantly higher proportion of oocytes with dispersed CCs compared to oocytes failed to mature (91.3% vs. 55.6%, p=0.023). There were no significant differences of oocytes outer ($155.7{\mu}m$ vs. $152.4{\mu}m$, NS), inner ($114.3{\mu}m$ vs. $113.4{\mu}m$, NS) diameters and zona thicknesses ($41.3{\mu}m$ vs. $39.1{\mu}m$, NS) between matured and not-matured oocytes. In-vitro maturation rate of oocytes with dispersed CCs was significantly higher than which of oocytes with compacted CCs (67.7% vs. 20.0%, p=0.044). Oocyte diameters (outer and inner) and thicknesses were not related with maturational competence. Conclusion: Our results suggest that in vitro maturational competence of GV stage oocytes obtained from stimulated IVF cycles is closely associated with the cumulus morphology but not oocyte diameter.
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제안 방법
In this report, we collected human GV stage oocytes from stimulated IVF cycles and prospectively investigated a relationship between cumulus morphology as well as oocyte diameter and their in vitro maturational competence.
데이터처리
The Student's t test was used to compare parametrical variables between two groups and Chi-square test was used for the comparison of two proportions.
성능/효과
11 found mRNA expression of the LH receptor in the CCs of COCs obtained from unstimulated cycles; the CCs expressing the LH receptors were mainly dispersed, and no or very few LH receptors could be detected in compacted and sparse CCs. From the findings, it can be postulated that oocytes with dispersed CCs, even though not matured despite stimulation, are in more advanced stage of development than those with compacted CCs.
In conclusion, the cumulus morphology of GV stage oocyte obtained from stimulated IVF cycle was closely associated with their in vitro maturational competence after denudation. This finding might be helpful in predicting in vitro maturational potential of immature oocytes from stimulation cycles.
후속연구
In the present study, we did not evaluate the qualities of in vitro matured metaphase II oocytes with regards to their developmental competence. For providing more useful information, further study about the developmental competence of in vitro matured oocytes would be needed. Human metaphase II oocytes have distinct morphological characteristics that may be indicative of quality and thus could be used to predict embryo quality and implantation potential.
Selection of GV stage oocytes with dispersed CCs could be one of strategies to overcome lower developmental potential of human immature oocyte. Further fundamental researches are needed with regards to the relevance of cumulus type and its biological interaction with oocytes to achieve better developmental competence of immature oocytes.
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