[논문]Antithrombotic Compounds form the Leaves of Ligularia stenocephala M.

Yoon, Min-Ho; Cho, Chong-Woon; Lee, Jae-Won; Kim, Young-Shin; An, Gil-Hwan; Lim, Chi-Hwan

Antithrombotic Compounds form the Leaves of Ligularia stenocephala M. 원문보기

Natural product sciences, v.14 no.1, 2008년, pp.62 - 67

Yoon, Min-Ho (Department of Bio Environmental Chemistry, College of Agriculture, Chungnam National University) , Cho, Chong-Woon (Department of Bio Environmental Chemistry, College of Agriculture, Chungnam National University) , Lee, Jae-Won (Department of Bio Environmental Chemistry, College of Agriculture, Chungnam National University) , Kim, Young-Shin (Department of Bio Environmental Chemistry, College of Agriculture, Chungnam National University) , An, Gil-Hwan (Department of Food Science and Technology, College of Agriculture, Chungnam National University) , Lim, Chi-Hwan (Department of Bio Environmental Chemistry, College of Agriculture, Chungnam National University)

Abstract ▼ AI-Helper

The leaf extract of Ligularia stenocephala showed the highest anti-platelet aggregating activity in large numbers of edible and herbal plants. The active fraction fractionationed from L. stenocephala extract by using preparative HPLC inhibited the platelet aggregation up to above 80% and its blood coagulating time (PT and APTT) also showed similar effect to aspirin (0.2 ${\mu}g/mL$), known as an anti-thrombus compound. Two antithrombus active compounds were purified and identified as 3,5-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid, respectively, on the basis of NMR and FAB-MS spectroscopic data. Two active compounds has not only antiplatelet aggregating activity, but also has anticoagulating activity.

주제어

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문제 정의

The authors have isolated two phenylpropanoid compounds from the EtOAc-soluble fraction of the leaves of L stenocephala and tested the antithrombus activity. This paper describes the structural elucidation of two active compounds (1 - 2) and their biological activity.

제안 방법

Mass spectra were measured by using a Jeol JMS-BX 303 spectrometer in the FAB mode using glycerol matrix with polyethylene glycol as internal standard. The and 13C-NMR spectra were determined on a Vhrian UNITY 300 (300 MHz for iH-NMR and 75 MHz for 13C-NMR) in CD3OD, using the tetramethylsilane (TMS) signal as an internal standard. Analytical silica gel TLC (Kiesel gel 60 F254, 0.

이론/모형

Estimation of antiplatelete aggregating activity (modified smear method) - Modified thin smear method was performed for measuring antiplatelete aggregating activity (Yun-Choi et al.f 1985). The test samples were dissolved in saline, while H₂O-insoluble samples were first dissolved in EtOH and then diluted in saline to give 1% of the final EtOH concentration.

참고문헌 (19)

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원문보기 상세보기
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