[논문]Effects of Non-saponin Red Ginseng Components on the Function of Brain Cells

Sohn, Eun-Hwa; Do, Hang; Kang, Nam-Sung; Jang, Seon-A; Park, Sul-Kyung; Lee, Hye-Rim; Rhee, Dong-Kwon; Pyo, Suhk-Neung

doi:10.5142/jgr.2008.32.1.062

Effects of Non-saponin Red Ginseng Components on the Function of Brain Cells 원문보기

Journal of ginseng research = 高麗人參學會誌, v.32 no.1, 2008년, pp.62 - 66

Sohn, Eun-Hwa (Department of Herbal Medicine Resource, Kangwon National University) , Do, Hang (College of Pharmacy, Sungkyunkwan University) , Kang, Nam-Sung (College of Pharmacy, Sungkyunkwan University) , Jang, Seon-A (Department of Herbal Medicine Resource, Kangwon National University) , Park, Sul-Kyung (Department of Herbal Medicine Resource, Kangwon National University) , Lee, Hye-Rim (College of Pharmacy, Sungkyunkwan University) , Rhee, Dong-Kwon (College of Pharmacy, Sungkyunkwan University) , Pyo, Suhk-Neung (College of Pharmacy, Sungkyunkwan University)

Abstract ▼ AI-Helper

Non-saponin gingseng fraction components (NSRG) have been known to have a variety of biological activity. However, the effects of these components on the function of brain cell have not been characterized in detail. In this study, we investigated the preventive effect of non-saponin red ginseng components on acrylamide (ACR)-induced suppression of neural cell adhesion molecule (NCAM), which is highly expressed in neuronal cells. The data showed that NSRG blocked the suppression of NCAM expression by ACR in neuroblastoma cells (SK-N-SH). In addition, NSRG significantly increased NCAM expression in ACR-nontreated neuroblastoma cells. NSRG treatment resulted in the increase of cell proliferation in a concentration-dependent manner. We also examined whether NSRG could modulate the NO production of astrocytes. When glioma cells (C6) were treated with various concentrations of NSRG (100-300 ug/ml) in the presence or absence of $IFN-{\gamma}$ for 24 hours, NO production was suppressed in $IFN-{\gamma}-$stimulated C6 cells. Taken together, these results demonstrate that treatment of brain cells with NSRG results in the enhancement of proliferation, the suppression of NO production and the protective effect on NCAM expression impaired by ACR. Thus, the present data suggest that NSRG has proliferative and neuroprotective effects and these effects could be useful in neuronal diseases.

주제어

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문제 정의

The aim of this study was to investigate the biological effects of NSRG on the expression of NCAM in neuron cells impaired by acrylamide (ACR) and IFN-y-induced NO production in glial cells.

대상 데이터

Six-year Korean red ginseng was obtained from the Korean Society of Ginseng. 100 g of 6-year red ginseng was extracted three times with 1000 ml of MeOH for 4 h under reflux.

데이터처리

The data is represented as a mean± S.E. The statistical difference between the groups was determined using a one-way analysis of variance (ANOVA) with a Student- Newman-Keuls test. A P value < 0.

이론/모형

SK-N-SH neuro blastoma cells (5x10⁴ cells/well) were treated with various concentrations of NSRG in the presence of acrylamide (3 mM) for 24 h. Cell proliferation was determined by MTT assay. Optical density was measured at 540 nm.
Cell proliferation was determined using a 3-(4, 5-dime- thylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay adapted from Mosmann (1983)8). Briefly, the cells were seeded in 96-well plates at a suitable densities of cells per well with various concentrations of NSRG for 24 h.

참고문헌 (15)

Nam, K.Y. : The comparative understanding between red ginseng and white ginsengs, processed ginseng (panax ginseng C.A. Mayer). Korea J. Ginseng Sci. 29, 1-18 (2005).

원문보기 상세보기
Okuda, H., Lee, S.D., Matsuura, Y., Zheng, Y., Sekiya, K., Takaku, T., Kameda, K., Hirose, K., Ohtani, K., Tanaka, O. and Sakata, T. : Biological activities of non-saponin compounds isolated from Korea Red Ginseng. Korea J. Ginseng Sci. 14 (2), 157-161 (1990).
Lee, S.D., Lee, K.S., Okuda, H. and Hwang, W.I. : Inhibitory effect of crude acidic polysaccharide of Korean ginseng on lipolutic action of toxohormone-L from cancerous ascites fluid. Korea J. Ginseng Sci. 14, 10-13 (1990).
Okuda, H. : Biological activities of non-saponin compounds isolated from Korean red ginseng. Proc. Int'l Ginseng Symp. on Korean Ginseng. p. 15-19 (1990).
Larina, L., Cho, B.G., Ten, L. and Park, H. : Isolation of Saponin-free Fraction from Ginseng (Panax Ginseng C.A. Meyer) and its Effects on the Function of Neutrophils. Soluble Non Saponin Constituents from Korean Red Ginseng (Panax ginseng C.A. Meyer): Immunomodulating Activities of Acidic Polysaccharide, Proc. of 99 Korea-Japan Ginseng Symp., p.191 (1999).
Lauralee Sherwood, Human Physiology. p. 136-137. Thomson Learning Publisher, Belmont (2004).
Suzuki, M., Angata, K., Nakayama, J. and Fukuda, M. : Polysialic acid and mucin type o-glycans on the neural cell adhesion molecule differentially regulate myoblast fusion. J. Biol. Chem. 278(49), 49459-49468 (2003).

상세보기
Mosmann, T. : Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 65, 55-63 (1983).

상세보기
Ding, A.H., Nathan, C.F. and Stuer, D.J. : Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J. Immunol. 141(7), 2407-2412 (1988).

상세보기
Whitton, P.S. : Inflammation as a causative factor in the aetiology of Parkinson's disease. Br. J. Pharmacol. 150(8), 963- 976 (2007).

상세보기
Akama, K.T. and Van Eldik, L.J. : Beta-amyloid stimulation of inducible nitric-oxide synthase in astrocytes is interleukin- 1beta- and tumor necrosis factor-alpha (TNFalpha)-dependent, and involves a TNF alpha receptor-associated factorand NFkappaB-inducing kinase-dependent signaling mechanism. J. Biol. Chem. 275(11), 7918-7924 (2000).

상세보기
Price, D.L. : New perspectives on Alzheimer's disease. Annu. Rev. Neurosci. 9, 489-512 (1996).

상세보기
Abbas, N., Bednar, I., Mix, E., Marie, S., Paterson, D., Ljungberg, A., Morris, C., Winblad, B., Nordberg, A. and Zhu, J. : Up-regulation of the inflammatory cytokines IFNgamma and IL-12 and down-regulation of IL-4 in cerebral cortex regions of APP(SWE) transgenic mice. J. Neuroimmunol. 126(1-2), 50-57 (2002).

상세보기
Shinoda, J. and Whittle, I.R. : Nitric oxide and glioma: a target for novel therapy ?. Br. J. Neurosurg. 15(3), 213-220 (2001).

상세보기
Park, K.M., Kim, Y.S., Jeong, T.C., Joe, C.H., Shin, H.J., Lee, Y.H., Nam, K.Y. and Park, J.D. : Nitric oxide is involved in the immunomodulating activities of acidic polysaccharide from Panax ginseng. Plana Med. 67, 122-126 (2001).

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