목적 : 본 논문에서는 경락을 추적하기 위한 피내(皮內) 알시안 블루(Alcian blue) 염색 방법을 기술한다. 방법 : 1% 알시안 블루 용액을 31게이지 바늘이 달린 0.5mL 인슐린 주사기를 사용하여 경혈 지점에 피내 주사한 후 실체 현미경하에서 수술, 관찰하였다. 면역조직화학적 방법을 사용하여 해당 조직을 레이저 공초점주사현미경으로 관찰하였다. 결과 : 알시안 블루로 염색되고 피하로 이어지는 실 모양 구조물을 관찰하였다. 이 조직 내에서 특징적인 막대모양 핵 배열 및 $1-2{\mu}m$크기의 데옥시리보핵산 입자가 관찰되었다. 또한 경혈 조직 내에서 풍부한 모세혈관총 및 말초신경 말단, 그리고 약 $300{\mu}m$크기의 소체형 구조물을 확인하였다. 결론 : 경혈 지점의 피내에서 발견된 특징적 실 모양 구조물 및 소체형 구조물은 각각 표층 봉한관 및 소체로 판단된다.
목적 : 본 논문에서는 경락을 추적하기 위한 피내(皮內) 알시안 블루(Alcian blue) 염색 방법을 기술한다. 방법 : 1% 알시안 블루 용액을 31게이지 바늘이 달린 0.5mL 인슐린 주사기를 사용하여 경혈 지점에 피내 주사한 후 실체 현미경하에서 수술, 관찰하였다. 면역조직화학적 방법을 사용하여 해당 조직을 레이저 공초점주사현미경으로 관찰하였다. 결과 : 알시안 블루로 염색되고 피하로 이어지는 실 모양 구조물을 관찰하였다. 이 조직 내에서 특징적인 막대모양 핵 배열 및 $1-2{\mu}m$크기의 데옥시리보핵산 입자가 관찰되었다. 또한 경혈 조직 내에서 풍부한 모세혈관총 및 말초신경 말단, 그리고 약 $300{\mu}m$크기의 소체형 구조물을 확인하였다. 결론 : 경혈 지점의 피내에서 발견된 특징적 실 모양 구조물 및 소체형 구조물은 각각 표층 봉한관 및 소체로 판단된다.
Objective In this article, we report on the intradermal Alcian blue staining method for tracing the meridians of acupuncture. Methods 1% Alcian blue solution was injected into acupoints by using a 0.5mL insulin syringe with a 31-gauge needle, then the skin was incised and was observed under a stereo...
Objective In this article, we report on the intradermal Alcian blue staining method for tracing the meridians of acupuncture. Methods 1% Alcian blue solution was injected into acupoints by using a 0.5mL insulin syringe with a 31-gauge needle, then the skin was incised and was observed under a stereoscopic microscope. The specimens were examined by using immunohistochemical methods and were observed under a confocal laser scanning microscope. Results A threadlike structure, which was visualized with Alcian blue, existed in dermis layer and proceeded to hypodermis. In this structure, characteristic alignments of rod- shaped nuclei and $1-2{\mu}m$ sized DNA granules were observed. Furthermore, abundant blood capillary plexuses, peripheral nerve endings, and a corpuscle-like structure(about $300{\mu}m$ in diameter) were visualized in the skin tissues of acupoints. Conclusion It was concluded that the specific threadlike and corpuscle-like structures corresponded to superficial Bonghan duct and corpuscle, respectively.
Objective In this article, we report on the intradermal Alcian blue staining method for tracing the meridians of acupuncture. Methods 1% Alcian blue solution was injected into acupoints by using a 0.5mL insulin syringe with a 31-gauge needle, then the skin was incised and was observed under a stereoscopic microscope. The specimens were examined by using immunohistochemical methods and were observed under a confocal laser scanning microscope. Results A threadlike structure, which was visualized with Alcian blue, existed in dermis layer and proceeded to hypodermis. In this structure, characteristic alignments of rod- shaped nuclei and $1-2{\mu}m$ sized DNA granules were observed. Furthermore, abundant blood capillary plexuses, peripheral nerve endings, and a corpuscle-like structure(about $300{\mu}m$ in diameter) were visualized in the skin tissues of acupoints. Conclusion It was concluded that the specific threadlike and corpuscle-like structures corresponded to superficial Bonghan duct and corpuscle, respectively.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
The samples were fixed in 4% paraformaldehyde (PFA) solution for 1 hour, washed with phosphate buffered solution(PBS), and then stained with DAPI solution(SlowFade Gold, Invitrogen, Oregon, USA) for 10minutes in a dark place. After staining, the samples were covered by a coverslip and examined under a phase contrast microscope (BX51, Olympus, Japan) at the excitation wavelength of 350nm for detection of DAPI fluorescence(emission wavelength: 470nm) from cell nuclei.
5g/kg) administered intraperitoneally. All experimental procedures including surgery were performed under deep anesthesia. The animals were kept in the sternal recumbency position.
To stain blood vessels, sections were incubated with mouse monoclonal RECA-1 antibody(diluted 1:50, 1 day, Abcam, UK ). Visualization was performed by using Alexa Flour 488-conjugated goat anti-mouse IgG(H+L)(diluted 1:500, 1 day, Invitrogen, USA). All incubations of thick sections with primary and secondary antibodies were carried out at 4℃.
대상 데이터
Male Wistar rats of 10 -12weeks old were used in this study. The animals were housed in a temperature-controlled environment(23℃) with 60% relative humidity with a 12-h light/dark cycle.
Two hours after injection, the dorsal skin was incised, from the LSP region to the scapula region, with operation knives and scissors. The stained parts of the skin were observed under a stereoscopic microscope(SZX 12, Olympus, Japan), and images were taken by a digital camera(Nikon, Japan) and a CCD camera(DP 70, Olympus, Japan). After observation, the Ab-stained regions were sampled for histologial analyses.
성능/효과
(c) Strong stainability with Alcian blue is known to be a general characteristic of BH ducts andcorpuscles(Fig. 1): it was reported that lymphatic and organ-surface thread like structures(BH ducts and corpuscles) have stronger affinity and stainability to Alcian blue compared with the other tissues. This means there exist abundant mucopolysaccarides(hyaluronans) in the threadlike structures, since the Alcian bule is known to form reversible electrostatic bonds between the cationic dye and the anionic sites on the polysaccarides18).
It was concluded that the threadlike structure was the superficial BH duct on the basis of characteristic alignments of rod-shaped nuclei and the existence of 1-2μm sized DNA granules in the tissue.
참고문헌 (25)
Sekhon GS. Acupuncture channels-myth or reality. Chin. Med. J. 1982;95(12):912-914
Deadman P, Al-Khafaji M, Baker K. A manual of acupuncture. The Journal of Chinese Medicine, East Sussex, 2001
Kim BH. On the Kyungrak system. J. Acad. Med. Sci. DPR Kor. 1963;90:1-35
Tiberiu R, Gheorghe G, Popescu I. Do meridians of acupuncture exist? A radioactive tracer study of the bladder meridian. Am. J. Acupunc. 1981;9(3):251-256
Lazorthes Y, Esquerr'e J-P, Simon J, Guiraud G, Guiraud R. Acupuncture meridians and radiotracers. Pain 1990;40:109-112
Lee B-C, Baik KY, Johng H-M, Nam TJ, Lee J, Sung B, Choi C, Park W-M, Park E-S, Park D-H, Yoon Y-S, Soh K-S. Acridine orange staining method to reveal characteristic features of intravascular threadlike structure. Anat. Rec. 2004;278B:27-30
Baik KY, Lee J, Lee B-C, Johng H-M, Nam TJ, Sung B, Cho S, Soh K-S Acupuncture meridian and intravascular Bonghan duct. Key 0Eng. Mater. 2005;277-279:125-129
Lee B-C, Yoo JS, Baik KY, Kim KW, Soh K-S. Novel threadlike structures(Bonghan ducts) inside lymphatic vessels of rabbits visualized with a Janus Green B staining method. Anat. Rec. 2005;286B:1-7
Johng H-M, Yoo JS, Yoon T-J, Shin H-S, Lee B-C, Lee C, Choi C, Sohn Y, Lee J-K, Soh KS. Use of magnetic nanoparticles to visualize threadlike structures inside lymphatic vessels of rats. eCAM 2007;4(1):77-82
Yoo JS, Johng H-M, Yoon T-J, Shin H-S, Lee B-C, Lee C, Ahn BS, Kang D-I, Lee J-K, Soh K-S. In vivo fluorescnec imaging of threadlike tissues(Bonghan ducts) inside lymphatic vessels with nanoparticles. Curr. Appl. Phys. 2006;7:342-348
Shin HS, Johng HM, Lee BC, Cho SI, Baik KY, Yoo JS, Soh KS. Feulgen reaction study of novel threadlike structures(Bonghan ducts) on the surface of mammalian organs. Anat. Rec. 2005;284B(1):35-40
Lee B-C, Yoo JS, Ogay V, Kim KW, Dobberste in H, Soh K-S, Chang B-S. Electron microscopic study of novel threadlike structures on the surfaces of mammalian organs. Microsc. Res. Tech. 2007;70:34-43
Yoo JS, Kim MS, Sung B, Lee BC, Soh KS, Lee SH, Kim YJ, Dobberstein H. Cribriform structure with channels in the acupuncture meridian-like system on the organ surfaces of rabbits. Acupunc. Electro-Therp. Res. 2007;32(1/2): 130-132
Soh K-S, Hong S, Hong J-Y, Lee B-C, Yoo JS. Immunohistochemical characterization of intravascular Bonghan duct. Microcirculation 2006;13:166
Sung B, Kim M-S, Lee B-C, Yoo JS, Lee S-H, Kim Y-J, Kim K-W, Soh K-S. Measurement of flow speed in the channels of novel threadlike structures on the surfaces of mammalian organs. Naturwissenschaften 2008;95:117-124
Ripellino JA, Klinger MM, Margolis RU, Margolis RK. The hyaluronic acid binding region as a specific probe for the localization of hyaluronic acid tissue section. Application to chickembryo and rat brain. J. Histochem. Cytochem. 1985;33:1060-1066
Ogay V, Baik KY, Lee BC, Soh K-S. Characterization of DNA-containing granules flowing through the meridian-like system on the internal organs of rabbits. Acupunc. Electro-Therap. Res. 2006;31:13-31
Kwon J, Baik KY, Lee B-C, Soh K-S, Lee NJ, Kang CJ. Scanning probe microscopy study of microcells from the organ surface Bonghan corpuscle. Appl. Phys. Lett. 2007;90:173903
※ AI-Helper는 부적절한 답변을 할 수 있습니다.