선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- Table 2. Oligonucleotide primers used for PCR in this study.
- PCR amplifications were done in a 20 圍 PCR PreMix (Bioneer Co., Korea) containing 10 mM Tris-HCl, 40 mM KCl, 1.5 mM MgCh, 250 “M dNTP, 1 unit of Taq polymerase. Amplifications were carried out in a PCR machine (ASTEC PC802) using an initial denaturation at 95 t for 5 min followed by 30 cycles (TNF-a, COX-1 : 35 cycles) of denaturation for 60 sec at 95 t, annealing for 60 sec at 52 t (COX-1, COX-2 : 55 t) and extension for 60 sec at 72 t.
대상 데이터
- Louis, MO). Dulbecco's Modified Eagle's Medium (DMEM) containing L-glutamine (200 mg/L) and FBS were purchased from Hyclone (Logan, UT). TNF ELISA kit (BD OptEIATM Rat TNF ELISA Set), IL-4 ELISA kit (BD OptEIATM Rat IL-4 ELISA Set) were purchased from BD Biosciences (Franklin Lakes, NJ).
데이터처리
- experiments. Comparisons between two groups were analyzed using Student’s t-test. P values less than 0.
이론/모형
- Cell viability was evaluated by MTT assay. Data represent the mean 士 SE of three independent experiments.
- TNF- a (A) and IL-4 (B) concentration was measured from cell supernatants using ELISA method. Vertical bars represent as the mean 士 SE from 4 wells.
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