Metacercariae of Acanthoparyphium marilae Yamaguti, 1934 (Digenea: Echinostomatidae) were discovered in an intertidal clam, Mactra veneriformis, in a southwestern coastal area of the Republic of Korea. A total of 128 metacercariae were detected from 10 clams examined. They were round, 320 m in avera...
Metacercariae of Acanthoparyphium marilae Yamaguti, 1934 (Digenea: Echinostomatidae) were discovered in an intertidal clam, Mactra veneriformis, in a southwestern coastal area of the Republic of Korea. A total of 128 metacercariae were detected from 10 clams examined. They were round, 320 m in average diameter, with 23 collar spines. They were fed experimentally to chicks, and 10 days later adult flukes were obtained. The adults were morphologically characterized by the head collar with a single row of 23 dorsally uninterrupted spines, without special end group spines, a round ventral sucker, 2 round and tandem testes, and vitellaria extending at lateral fields from the posterior extremity not beyond the middle level of the posterior testis. The most characteristic feature of this species was the limited distribution of vitellaria, which differs from Acanthoparyphium tyosenense Yamaguti, 1939, the metacercariae of which are encysted in the same mollusk species. This is the first report in which the metacercariae of this species were detected, and the intertidal bivalve, M. veneriformis, has been identified as a second intermediate host for A. marilae.
Metacercariae of Acanthoparyphium marilae Yamaguti, 1934 (Digenea: Echinostomatidae) were discovered in an intertidal clam, Mactra veneriformis, in a southwestern coastal area of the Republic of Korea. A total of 128 metacercariae were detected from 10 clams examined. They were round, 320 m in average diameter, with 23 collar spines. They were fed experimentally to chicks, and 10 days later adult flukes were obtained. The adults were morphologically characterized by the head collar with a single row of 23 dorsally uninterrupted spines, without special end group spines, a round ventral sucker, 2 round and tandem testes, and vitellaria extending at lateral fields from the posterior extremity not beyond the middle level of the posterior testis. The most characteristic feature of this species was the limited distribution of vitellaria, which differs from Acanthoparyphium tyosenense Yamaguti, 1939, the metacercariae of which are encysted in the same mollusk species. This is the first report in which the metacercariae of this species were detected, and the intertidal bivalve, M. veneriformis, has been identified as a second intermediate host for A. marilae.
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가설 설정
Description: Body elongated, tapering anteriorly, and rather elliptical at the posterior end. Head collar armed with 23 spines arranged in a single row, without ventral corner spines.
제안 방법
A hundred metacercariae were administered orally to 5 experimental chicks (3-day-old, Gallus domesticus), and the chicks were killed on day 10 post-infection (PI). Their small intestines were resected, longitudinally opened in a Petri dish containing saline, and examined for worms using a stereomicroscope. The adult flukes collected were then fixed in 10% neutral formalin under a cover glass pressure and stained with Semichon’s acetocarmine.
대상 데이터
The metacercariae of A. marilae, together with those of A. tyosenense and Himasthla alincia, were detected in the bivalve, M. veneriformis, which were purchased in a southwestern costal area, Buangun, Jeollabuk-do, the Republic of Korea (Table 1). The metacercariae were isolated from the muscles and gills of the bivalves with a sharp, pointed pin and a stereomicroscope.
성능/효과
A total of 128 A. marilae metacercariae were recovered from 10 M. veneriformis, with an infection rate of 100% and an average number of 12.8 metacercariae per clam (Table 1). The metacer cariae were coiled in thin-walled, oval to round cysts, 320 m in average diameter, with branched excretory tubules and granules, and with transparent cyst walls (Fig.
The worms revealed a prominent head collar with 23 cephalic spines arranged in a single row, without grouped corner spines, a long cirrus sac reaching beyond the posterior margin of the acetabulum, round and entire testes, and vitellaria distribution limited posteriorly to the level of the posterior testis. On the basis of these characteristics, the specimens were assigned to a species of Acanthoparyphium [14], and identified as A. marilae Yamaguti, 1934 [12].
In the species determination for our specimens, the level of vitellaria extension appeared to be the most salient clue. The vitellaria distribution of our specimens was confined to the middle level of the posterior testis; this feature was unique only to A. marilae, among the 14 species. In addition, testes morphology and the position of the ventral sucker were also useful features.
참고문헌 (16)
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