[논문]토양선충 Caenorhabditis elegans를 이용한 세리아(<tex>$CeO_2$</tex>) 독성연구

노지연; 박영권; 최진희

토양선충 Caenorhabditis elegans를 이용한 세리아($CeO_2$) 독성연구
Ecotoxicological Effects of $CeO_2$ Nanoparticles on Soil Nematode Caenorhabditis elegans 원문보기

환경독성학회지 = Journal of environmental toxicology, v.23 no.2, 2008년, pp.87 - 91

노지연 (서울시립대학교 환경공학부 독성학연구실) , 박영권 (서울시립대학교 환경공학부 독성학연구실) , 최진희 (서울시립대학교 환경공학부 독성학연구실)

Abstract ▼ AI-Helper

In this study, three different sizes of cerium oxide ($CeO_2$) nanoparticles were synthesized and exposed to Caenorhabditis elegans to investigate the potential harmful effect of $CeO_2$ nanoparticles on the environment. The effects of the $CeO_2$ nanoparticles on C. elegans were assessed at multiple levels, such as with respect to stress response gene expression, growth, reproduction and mortality. Moreover, to test the ecotoxicological relevance of $CeO_2$-induced gene expression. The overall results suggest that $CeO_2$ nanoparticles may provoke ecotoxicity in C. elegans especially with respect to gene expression, reproduction and survival, which can comprise an important contribution to knowledge on the ecotoxicity of $CeO_2$ nanoparticles, about which little data are available. This is particularly valuable in the biomarker research on ecotoxicology, as ecological relevance is a crucial criterion for the applicability of the biomarker in field biomonitoring and ecological risk assessment.

주제어

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제안 방법

All the tests were replicated at least three times, and the relative densities of each band were determined with the use of a Kodak EDAS 290 image analyzer (Kodak, Rochester, NY, USA), with a TFX-20.

데이터처리

The statistical differences between the control and treated worms were determined with the aid of the parametric t test.

이론/모형

RNA concentrations were determined by the absorbance at 260 nm. The two-step reverse transcription-polymerase chain reaction (RT-PCR) method was used with RT Premix (Bioneer Co., Seoul, Korea) and PCR Premix kits (Bioneer Co.), using a PTC-100 thermal cycler (MJ Research, Lincoln, MA, USA). The primers were designed on the basis of the sequences retrieved from the C.

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