[논문]강화사자발쑥의 마크로파지 RAW 264.7세포에 대한 Tumor Necrosis Factor-<tex>$\\alpha$</tex>, Prostaglandin <tex>$E_2$</tex>, Cyclooxygenase-2 및 LPS 유도 Nitric Oxide 생성 저해

윤준용; 최세영; 박표잠; 정해곤; 신흥묵; 석경호; 임병우

강화사자발쑥의 마크로파지 RAW 264.7세포에 대한 Tumor Necrosis Factor-$\\alpha$, Prostaglandin $E_2$, Cyclooxygenase-2 및 LPS 유도 Nitric Oxide 생성 저해
Extracts of Artemisia princeps Pampanini Inhibit Lipopolysaccharide-induced Nitric Oxide, Cyclooxygenase-2, Prostaglandin $E_2$, and Tumor Necrosis Factor-$\\alpha$ Production from Murine Macrophage RAW 264.7 Cells 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.16 no.5, 2008년, pp.326 - 331

윤준용 (건국대학교 의료생명대학 생명과학부) , 최세영 (건국대학교 의료생명대학 생명과학부) , 박표잠 (건국대학교 의료생명대학 생명과학부) , 정해곤 (강화농업기술연구센터) , 신흥묵 (동국대학교 한의과대학 생리학 교실) , 석경호 (경북대학교 의과대학 약리학교실) , 임병우 (건국대학교 의료생명대학 생명과학부)

Abstract ▼ AI-Helper

To search for immunoactive natural products exerting anti-inflammatory activity, we have evaluated the effects on the water extracts of Artemisia princeps Pampanini (APP) on lipopolysaccharide-induced nitric oxide (NO), tumor necrosis factor-$\alpha$ (TNF-$\alpha$), and prostaglandin $E_2$ ($PGE_2$) production by RAW 264.7 macrophage cell line. Our data indicate that this extract is a potent inhibitor of NO production and it also significantly decreased PGE2 and TNF-$\alpha$ production. Consistent with these results, the protein and mRNA expression level of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) was inhibited by water extracts of APP in a dose-dependent manner. These results suggest that APP may exert anti-inflammatory and analgesic effects possibly by suppressing the inducible NO synthase and COX-2 expressions.

주제어

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제안 방법

The cells were left untreated or treated with LPS (1 ㎍/㎖) and APP (25, 50, 75, 100 ㎍/㎖) for 24 h, and the production of NO (A) and PGE₂ (B) was evaluated by Griess reaction and EIA, respectively. L-N6 -(1-iminoethyl) lysine (L-NIL) (10 µM) or NS398 (10 µM) was used in the assay as a positive control (data not shown).
7 cells. To examine the effects of APP on the NO and PGE₂ production in RAW264.7 cells, cell culture medium was harvested, and the production of nitrite or PGE₂ was measured using the method of Griess reaction or EIA, respectively. APP reduced the LPS-induced NO and PGE₂ production in a dose-dependent manner (Fig.
To investigate whether the APP could inhibit LPSstimulated TNF-α mRNA and protein expression, cells were preincubated with APP for 1 h, and then treated with 1 ㎍/㎖ of LPS for 4 h (for TNF-α mRNA) or 24 h (for TNF-α protein).

대상 데이터

PCR primers used in this study are listed below and were purchased from Bioneer (Seoul, Korea): TNF-α 5-'GCGACGTGGAACTGGCCAGAAG-3' (5'-primer) 5'-TCCATGCCGTTGGCCAGGAGG-3' (3'-primer); iNOS 5'- CCTTGTTCAGCTACGCCTTC-3' (5'-primer) 5'-CTGAGGGC TCTGTTGAGGT-3' (3'-primer); COX-2 5'-TGCATGTGGCTG TGGATGTCAT-3' (5'-primer), 5'-CACTAAGACACCCGTC ATCTCCA-3' (3'-primer); β-actin 5'-TACAGGCTTGTCAC TCGAANTT-3', 5' CCTAGAANGCATTTGCGGTGCACGA TG-3'.

데이터처리

*p < 0.05, **p < 0.005 vs. LPStreated group; significance of difference between treated groups in Student’s t-test.
Statistical analysis was performed using Student’s t-test with one-way analysis of variance.

성능/효과

Results show that the water extract of APP inhibits the production of NO, PGE2, and TNF-α in the LPS-stimulated RAW 264.7 macrophages at least in part by interfering with the transcription and/or translation of iNOS, COX-2, and TNF-α genes.
, and TNF-α) and the expression levels of iNOS and COX-2 in LPS-activated macrophages. The results of the present study indicate that APP inhibits the induction of iNOS in LPS-activated murine macrophages at the transcriptional level and NO production was reduced by APP in a concentration-dependent manner. Also, iNOS protein levels in the LPS-activated cells were markedly reduced by APP as compared with untreated cells.

후속연구

These results clearly demonstrated the antiinflammatory effects of APP as well as the possibility of therapeutic use of APP. However, further studies on the precise mechanism of action and the isolation/characterization of active chemical constituents are needed.

참고문헌 (19)

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