Kim, Jeong-Soon
(National Agrobiodiversity Center, Rural Development Administration)
,
Gwang, Jae-Gyun
(National Agrobiodiversity Center, Rural Development Administration)
,
Park, Ki-Hun
(National Agrobiodiversity Center, Rural Development Administration)
,
Shim, Chang-Ki
(National Agrobiodiversity Center, Rural Development Administration)
A molecular survey was conducted to identify the presence of the bacterial blight resistance genes (Xa1, Xa4, xa5, xa13 and Xa21) in 86 accessions of aromatic rice obtained from germplasm. The results revealed that the resistance gene Xa4 (32.5%), Xa21 (17%), and xa5 (16%) were widely observed in te...
A molecular survey was conducted to identify the presence of the bacterial blight resistance genes (Xa1, Xa4, xa5, xa13 and Xa21) in 86 accessions of aromatic rice obtained from germplasm. The results revealed that the resistance gene Xa4 (32.5%), Xa21 (17%), and xa5 (16%) were widely observed in tested rice germplasm. Among tested rice germplasm, 49 accessions showed the presence of more than one of five R genes, and 37 accessions possessed none of the R gene. TALLi and 05-IRRi-M-46 showed the presence of Xa4, xa5, xa13 and Xa21. Rice race $415{\times}Ir352$ exhibited positive amplicon for the Xa1, Xa4, xa5 and Xa21. Hyangmibyeo1hos, Ir841-85-1-1-2 and Jasmine85 showed the positive amplicon for the Xa1, Xa4 and xa5 genes. Yekywin Yinkya Hmwe and Khao Dawk Mali105 showed the presence of Xa1, Xa4 and Xa21 gene. Masino Basmati showed the presence of xa5, xa13, Xa21 genes. Xa1 and Xa21 genes were noticed in Mihayngbyeo, Tarana Deshi, Mayataung and AZUCENA. Hyangmibyeo2ho, Basmati 6311 and Basmati405 possessed only two R genes such as Xa4 and xa5, and xa5 and xa13, respectively. The evaluation results of bacterial blight resistance genes in aromatic rice germplasm will help in breeding of multi disease resistant varieties.
A molecular survey was conducted to identify the presence of the bacterial blight resistance genes (Xa1, Xa4, xa5, xa13 and Xa21) in 86 accessions of aromatic rice obtained from germplasm. The results revealed that the resistance gene Xa4 (32.5%), Xa21 (17%), and xa5 (16%) were widely observed in tested rice germplasm. Among tested rice germplasm, 49 accessions showed the presence of more than one of five R genes, and 37 accessions possessed none of the R gene. TALLi and 05-IRRi-M-46 showed the presence of Xa4, xa5, xa13 and Xa21. Rice race $415{\times}Ir352$ exhibited positive amplicon for the Xa1, Xa4, xa5 and Xa21. Hyangmibyeo1hos, Ir841-85-1-1-2 and Jasmine85 showed the positive amplicon for the Xa1, Xa4 and xa5 genes. Yekywin Yinkya Hmwe and Khao Dawk Mali105 showed the presence of Xa1, Xa4 and Xa21 gene. Masino Basmati showed the presence of xa5, xa13, Xa21 genes. Xa1 and Xa21 genes were noticed in Mihayngbyeo, Tarana Deshi, Mayataung and AZUCENA. Hyangmibyeo2ho, Basmati 6311 and Basmati405 possessed only two R genes such as Xa4 and xa5, and xa5 and xa13, respectively. The evaluation results of bacterial blight resistance genes in aromatic rice germplasm will help in breeding of multi disease resistant varieties.
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제안 방법
, 1974) in 1991 and had resistance to KI, K2 andK3 races. The aim of the present study was to get the information about the genetic diversities of bacterial blight resistance genes in aromatic rice germplasm to improve the aromatic rice breeding efficiency using several SNP and STS markers.
대상 데이터
(Table 1). All the STS and SNP markers used in this study were obtained from the Oligo Synthesis Facility, Bioneer Co., Korea.
Eighty six accessions of aromatic rice were obtained from National Agrobiodiversity Center, Rural Development Administration (RDA), and Republic of Korea and were listed in Table 2. Five rice seeds were imbibed for 4 h in 2% NaOCl further transferred to another container containing tap water for overnight and placed moist filter paper on Petri-dish at 30℃ for one-week in light condition.
Out of 28 accessions of japonica type aromatic rice, seven accessions (Flores, Inaguhu, JC149, Kalo Masino Dhan, Milagrosa mutant, TALLI, and 415XIr352) showed the BB resistant fragments linked to Xa4 gene. Positive amplicon (150 bp) for Xa4 gene was observed in 19 of 56 races of indica type aromatic rice (Arc6011, Aroma, AZUCENA, Basmati370, Basmati 9-93, Basmati 198, Basmati 6129, Basmati6311, Dal3, DS20, Jasmine85, Ir841-85-1-1-2, Khao Dawk Malil05, Tarana Deshi, Mayataung, Milfor6, Yekywin Yinkya Hmwe, and 05-Irri-M-46) (Fig. 2, Table 2).
이론/모형
The PCR analysis was conducted based on procedures described by Bioneer PCR Pre-mix kit manual (Bioneer Co. Ltd, Korea). The PCR reaction mixture contained 50 ng template DNA, 20 pmol of each primer set, 0.
성능/효과
In this study, eight of eighty six accessions of aromatic rice showed the presence of BB resistance gene linked to Xal and four accessions of aromatic rice (Kemiri, Khao Dawk Mali 105, Mayataung, and Padi danat) exhibited the presence the heterozygous BB resistance gene linked to Xal. Bacterial blight resistance gene ^2/ was first reported by Sakaguchi (1967) and mapped it on rice chromosome 4 and cloned recently by a map-based cloning strategy (Yoshimura, et al.
후속연구
The present study demonstrated the presence of multiple bacterial blight resistance genes in aromatic rice germplasm stored at National Agro-biodiversity center of Republic of Korea. In future, the results of the present study will be exploited for breeding programs to develop a multi disease resistant aromatic rice varieties against Xantomaonas oryzae pv. oryzae.
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