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선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- 2004). In this study, mRNA expression profiles from Masan Bay were compared with the reference site Gang- neung using the ACP-based PCR with 9 arbitrary primers. As shown in Fig.
- The status of environmental contamination of Masan Bay was determined by identifying DEGs in the brain of L. yokohamae as compared with those of Gangneung, Korea, using ACP-based PCR. Recently, the "omics" approach such as transcriptomics has been used to aid the risk assessment of pollutants and to assess the biological effect of toxicants on living organisms, which can offer an opportunity to identify a potential biomarker and gain a more comprehensive assessment (Waring et al.
이론/모형
- DEGs were screened by ACP-based PCR method (Kim et al. 2004) using the GeneFishing™DEG kits (Seegene, Korea). Briefly, the second-strand cDNA synthesis was conducted at 50℃ during one cycle of the first-stage PCR in a final reaction volume of 20 μL containing 3 ~ 5 μL (about 50 ng) of diluted first-strand cDNA, 1 μL of dT-ACP2 (10 |1M), 1 μL of 10 μM arbitrary ACP, and 10 |1L of 2 x Master Mix (Seegene).
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