14종의 인산용해미생물을 근권으로부터 분하였고, 16S rRNA gene 염기서열에 의하여 동정하였다 그 중 hydroxyapatite를 첨가한 배지에서 인산용해능력이 가장 뛰어난 Acinetobacter sp., Pseudomonas orientalis, Enterobacter asburiae 3종을 선택하였다. 선택된 3종의 미생물에 의해 용해된 인산의 농도는 $200\;mg\;L^{-1}$에서부터 $2300\;mg\;L^{-1}$까지 이르렀으며, 증가된 인산 농도는 배양액의 pH와 역으로 비례하였다. HPLC를 사용하여 유기산을 측정한 결과 Acinetobacter sp.는 gluconic acid를, P. orientalis는 gluconic acid와 2-ketogluconic acid를 그리고 E. asburiae는 acetic acid와 succinic acid를 분비하였다. 한편 P. orientalis와 E. asburiae는 각각 $372\;mg\;L^{-1}$와 $191\;mg\;L^{-1}$의 IAA 분비하였고, Acinetobacter sp.는 IAA를 생성하지 못했다. 인산용해미생물이 오이의 생장에 미치는 효과를 조사한 결과, P. orientalis를 처리한 시험구가 가장 높았고, E. asburiae, Acinetobacter sp., control 순으로 나타났다. 이러한 식물생장 효과는 인산용해미생물에 의한 불용성 인산용해와 IAA 생산과 관련이 있다고 생각된다.
14종의 인산용해미생물을 근권으로부터 분하였고, 16S rRNA gene 염기서열에 의하여 동정하였다 그 중 hydroxyapatite를 첨가한 배지에서 인산용해능력이 가장 뛰어난 Acinetobacter sp., Pseudomonas orientalis, Enterobacter asburiae 3종을 선택하였다. 선택된 3종의 미생물에 의해 용해된 인산의 농도는 $200\;mg\;L^{-1}$에서부터 $2300\;mg\;L^{-1}$까지 이르렀으며, 증가된 인산 농도는 배양액의 pH와 역으로 비례하였다. HPLC를 사용하여 유기산을 측정한 결과 Acinetobacter sp.는 gluconic acid를, P. orientalis는 gluconic acid와 2-ketogluconic acid를 그리고 E. asburiae는 acetic acid와 succinic acid를 분비하였다. 한편 P. orientalis와 E. asburiae는 각각 $372\;mg\;L^{-1}$와 $191\;mg\;L^{-1}$의 IAA 분비하였고, Acinetobacter sp.는 IAA를 생성하지 못했다. 인산용해미생물이 오이의 생장에 미치는 효과를 조사한 결과, P. orientalis를 처리한 시험구가 가장 높았고, E. asburiae, Acinetobacter sp., control 순으로 나타났다. 이러한 식물생장 효과는 인산용해미생물에 의한 불용성 인산용해와 IAA 생산과 관련이 있다고 생각된다.
Fourteen bacterial strains were isolated from crop rhizosphere and identified as phosphate solubilizing bacteria (PSB) by 16S rRNA analysis. Only 3 strains exhibited a strong ability to solubilize insoluble phosphate in agar medium containing a hydroxyapatite. The rates of P solubilization by isolat...
Fourteen bacterial strains were isolated from crop rhizosphere and identified as phosphate solubilizing bacteria (PSB) by 16S rRNA analysis. Only 3 strains exhibited a strong ability to solubilize insoluble phosphate in agar medium containing a hydroxyapatite. The rates of P solubilization by isolates were ranged from 200 and $2300\;mg\;L^{-1}$, which are inversely correlated with pH in culture medium. Furthermore, HPLC analyses reveal the production of organic acid from the culture filtrates of PSB. Among these, strain Acinetobacter sp. released only gluconic acid, Pseudomonas orientalis produced gluconic acid which was subsequently converted into 2-ketogluconic acid, and Enterobacter asburiae released acetic acid and succinic acid. On the other hand, P. orientalis and E. asburiae released $372\;mg\;L^{-1}$ and $191\;mg\;L^{-1}$ of IAA into broth culture, respectively, while Acinetobacter sp. did not produce IAA. Furthermore, in vivo study showed that plant growth promoting effect by bacteria generally seemed to be increased IAA production and phosphate solubilization.
Fourteen bacterial strains were isolated from crop rhizosphere and identified as phosphate solubilizing bacteria (PSB) by 16S rRNA analysis. Only 3 strains exhibited a strong ability to solubilize insoluble phosphate in agar medium containing a hydroxyapatite. The rates of P solubilization by isolates were ranged from 200 and $2300\;mg\;L^{-1}$, which are inversely correlated with pH in culture medium. Furthermore, HPLC analyses reveal the production of organic acid from the culture filtrates of PSB. Among these, strain Acinetobacter sp. released only gluconic acid, Pseudomonas orientalis produced gluconic acid which was subsequently converted into 2-ketogluconic acid, and Enterobacter asburiae released acetic acid and succinic acid. On the other hand, P. orientalis and E. asburiae released $372\;mg\;L^{-1}$ and $191\;mg\;L^{-1}$ of IAA into broth culture, respectively, while Acinetobacter sp. did not produce IAA. Furthermore, in vivo study showed that plant growth promoting effect by bacteria generally seemed to be increased IAA production and phosphate solubilization.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
Acinetobacter sp., P orientalis, and E. asburiae was carried out at 1, 2, 5, 7 and 10 days after cultivation by HPLC analysis. The HPLC analysis of these culture filtrate revealed that four major peaks such as 1, 2, 3 and 4 were represented as gluconic acid, 2-ketogluconic acid, succinic acid, and acetic acid, ?respectively by comparing the retention times with those of the authentic standards (Fig.
Each pot was filled with 500 g of the mixture where a sterilized seed was placed. Five milliliters of each bacterial culture suspension (P. orientalis, E. asburiae, and Acinetobacter sp., 4 x 107 CFU ml-1) was amended to the rhizosphere of each plant in each treatment at 5 and 6 weeks after sowing and control plants received the same amount of culture broth (without bacterial inoculation). After 7 weeks, cucumber plants were harvested and fresh/dry weight of shoot and root, and N and P content in plant were measured in triplicate.
Growth promotion in cucumber caused by PGPR treatment may be due to the production of phytohormones. In this study, we have been able to identify IAA from the culture filtrate of PSB. Treatment of strain P.
shaker at 200 rpm. Samples were taken at 1, 2, 5, 7 and 10 days for the measurement of pH, P solubilization and organic acid concentration. All these experiments were carried out in triplicate.
, Chungbuk, Korea). The nucleotide sequence of the 16S rRNA gene was determined by Biodye Terminator cycle sequencing kit and compared with published 16S rRNA sequences using Blast search at Gene Bank Data base of NCBI (Bethesda, MD) in order to establish the identity of the isolates.
대상 데이터
Rhizospheres were taken at a depth of 15-20 cm from crop field in Chonnam Province, Republic of Korea. Diluted soils by sterile water at a rate of 10-5 were inoculated on hydroxyapatite (HY) agar medium (glucose 10 g; MgSO4 .
This study was supported by the Korea Research Foundation second step Brain Korea 21 project, Republic of Korea.
성능/효과
, 1997). This investigation suggested that the strain P. orientalis was an efficient candidate for plant growth promoting function by the instance of IAA production. Moreover, P2O5 and total nitrogen (T-N) of P.
참고문헌 (32)
Azcon, R., G. Azcon, D. Aghilar, and J.H. Barea. 1978. Effect of plant hormones present in bacterial cultures on the formation and response to vamendomycorrhiza. New Phytol. 80:359-364
Banik, S., and B.K. Dey. 1982. Available phosphate content of and alluvial soil as influenced by inoculation of some isolated phosphate solubilizing bacteria. Plant Soil. 69:353-364
Barea, J.M., E. Navarro and E. Montoya. 1976. Production of plant growth regulators by rhizosphere phosphate-solubilizing bacteria. J. Appl. Bact. 40:129-134
Benizri, E., A. Courtade, C. Picard and A. Guckert. 1998. Role of maize exudates in the production of auxins by Pseudomonas Fluorescens M.3.1. Soil Biol. Biochem. 30:1481-1484
Cheryl, L.P., and R.G. Bernard. 2002. Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Appl. Environ. Microbiol. 68:3795-3801
Datta, M., S. Banik and R.K. Gupta. 1982. Studies on the efficacy of a phytohormone producing phosphate solubilizing Bacillus firmus in augmenting paddy yield in acid soils of Nagaland. Plant and Soil 69:365-373
Freitas, J.R., M.R. Banerjee and J.J. Germida. 1997. Phosphatesolubilizing rhizobacteria enhance the growth and yield but not phosphorus uptake of canola (Brassica nupus L.). Biol. Fertil. Soils. 24:358-364
Gaur, A.C., and K.P. Ostwal. 1972. Influence of phosphate dissolving Bacilli on yield and phosphate uptake of wheat crop. Indian J. Exp. Biol. 10:393-394
Goldstein, A.H. 1986. Bacterial mineral phosphate solubilization:Historical perspective and future prospects. Am. J. Altern. Agric. 1:57-65
Goldstein, A.H., K. Braverman and N. Osorio. 1999. Evidence for mutualism between a plant growing in a phosphate-limited desert environment and a mineral phosphate solubilizing (MPS) rhizobacterium. FEMS Microbiol. Ecol. 30:295-300
Hu, H.Q., J.Z. He, X.Y. Li, and F. Liu. 2001. Effect of several organic acids on phosphate adsorption by variable charge soils of central China. Environ. Int. 26:353-358
Hussain, A., and V. Vancura. 1970. Formation of biologically active substances by rhizosphere bactieria and their effect on plant growth. Folia Microbiol. 15:468-478
Hwangbo, H., D.R. Park, W.Y. Kim, Y.S. Rim, H.K. Park, H.T. Kim, J. SunSuh and Y.K. Kim. 2003. 2-Ketogluconic acid production and phosphate solubilization by Enterobacter intermedium. Curr. Microbiol. 47:87-92.
Illmer, P., A. Barbato, and F. Schinner. 1995. Solubilization of hardly-soluble AlPO4 with P-solubilizing microorganisms. Soil Biol. Biochem. 27:265-270
Jones, D.L., and P.R. Darrah. 1994. Role of root derived organic acids in the mobilization of nutrients from the rhizosphere. Plant and Soil 166:247-257
Kim, K.Y., D. Jordan and H.B. Krishnan. 1997a. Rahnella aquatilis, a bacterium isolated from soybean rhizosphere can solubilize hydroxyapatite. FEMS Microbiol. Lett. 153:273-277
Kim, K.Y., G.A. McDonald and D. Jordan. 1997b. Solubilization of hydroxyapatite by Enterobacter agglomerans and cloned Escherichia coil in culture medium. Biol. Fertil. Soils. 24:347-352
Kloepper, J.W., K. Lifshitz and M.N. Schroth. 1988. Pseudomonas inoculants to benefit plant production. ISI Atlas Sci. Anim. Plant. 60-64
Lindow, S.E., C. Desurmont, R. Elkins, G. McGourty, E. Clark and M.T. Brandl. 1998. Occurrence of indole-3-acetic acid producing bacteria on pear trees and their association with fruit russet. Phytopathology 88:1149-1157
Liu, S.T., L.Y. Lee, C.Y. Tai, C.H. Horng, Y.S. Chang, J.H. Wolfram, R. Rogers and A.H. Goldstein. 1992. Cloning of and Erwinia herbicola gene necessary for gluconic acid production and enhanced mineral phosphate solubilization in Escherichia coli HB101. J. Bacterial. 174:5814-5819
Moghimi, A., and M.E. Tate. 1978. Does 2-ketogluconate chelate calcium in the pH range 2.4 to 6.4. Soil Biol. Biochem. 10:289-292
Satter, M.A., and A.C. Gaur. 1987. Production of auxins and gibberellins by phosphate dissolving microorganisms. Zentralbl. Microbiol. 142:393-395
Shekhar, N.C., S. Bhadauria, P. Kumar, H. Lal, R. Mondal and D. Verma. 2000. Stress induced phosphate solubilization in bacteria isolated from alkaline soils. FEMS Microbil. Lett. 182:291-296
Vandana, K., and G. Reeta. 2003. Solubilization of inorganic phosphate and plant growth promotion by cold tolerant mutants of Pseudomonas fluorescens. Microbiol. Res. 158:163-168
Olsen, S.R., and L.E. Sommers. 1982. Phosphorus. p. 403-430. In A.L. Page et al. (ed.), Method of soil analysis, Chemical and microbial properties. American Society of Agronomy, Madison, WI, U.S.A.
Yadav, K.S., and K.R. Dadarwal. 1997. Phosphate solubilization and mobilization through soil microorganisms. p. 293-308. In K.R. Dadarwal, (ed.), Biotechnological approaches in soil microorganisms for sustainable crop production. Scientific Publishers, Jodhpur, India
※ AI-Helper는 부적절한 답변을 할 수 있습니다.