[논문]Karyomorphological Studies on the Genus Spirogyra Link (Conjugales, Chlorophyta) from Korea

Kim, Jee-Hwan; Chaudhary, B.R.; Kim, Young-Hwan; Lee, In-Kyu

Karyomorphological Studies on the Genus Spirogyra Link (Conjugales, Chlorophyta) from Korea 원문보기

한국하천호수학회지= Korean journal of limnology, v.42 no.2, 2009년, pp.192 - 199

Kim, Jee-Hwan (Institute for Basic Sciences, Chungbuk National University) , Chaudhary, B.R. (Centre of Advanced Study in Botany, Banaras Hindu University) , Kim, Young-Hwan (Department of Biology, Chungbuk National University) , Lee, In-Kyu (School of Biological Science, Seoul National University)

Abstract ▼ AI-Helper

Freshwater green algae are one of the important sources of bioenergy in the future. Spirogyra is a conjugating filamentous zygnematacean green algal genus that is widely distributed worldwide with more than 400 species. Despite its widespread occurrence throughout the world, cytological studies of the genus have been limited. We investigated karyological features and chromosome numbers for seven Korean Spirogyra species. Most of the species examined in the present study showed significant karyological features, inner organization of nucleolus, heavily stainable nucleolar substance and the diffuse-centric nature of chromosomes, typical of the Conjugales. Chromosome number ranged from n=12 in S. varians to n=38 in S. africana. Aberrant cytokinesis resulted in binucleate and tetranucleate cells, which sometimes provide cytological explanation for different morphology and ploidal changes in clonal culture of Spirogyra or even different cells within the same filament. The present chromosome data also substantiates the earlier held assumption that aneuploidy must have been the chief driving force for speciation and evolution of the genus Spirogyra.

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가설 설정

Metaphase showing diffuse- or polycentric chromosomes embedded in the heavily stained nucleolar substance; 9. Anaphase showing daughter groups of chromosomes embedded in the stainable nucleolar material; 10. Telophase showing cytokinesis between the two daughter nuclei formed. Scale bars: 1, 5 and 6=50 μm; 2, 7 and 10=25 3, 4, 8 and 9=5 μm.

제안 방법

Squash preparations were made following Godwarde iron-alum acetocarmine technique (Godward, 1948, 1966). The preparations were examined, sealed with enamel and the suitable stages were photographed using light microscope(Olympus BH-2).

이론/모형

Double fixation, change of fixative after 1 hr of fixation, was done to completely remove the coloring matter from the filaments. Squash preparations were made following Godwarde iron-alum acetocarmine technique (Godward, 1948, 1966). The preparations were examined, sealed with enamel and the suitable stages were photographed using light microscope(Olympus BH-2).

성능/효과

A total of seven species, 16 populations, of Spirogyra were collected from various freshwater habitats in Korea during 1996 ~2006 (Table 1). The live cells were isolated under the dissecting microscope, washed thoroughly with sterile distilled water, and inoculated single filaments in the sterilized Woods Hole liquid medium (Nichols, 1973).
19) were determined in the present study. Out of the seven species, S, africana (n=36/38), S. decimina (n=23/24± 1), S. gracilis (n-24± 1) and S. varians (n=ca. 12) are reported for the first time. Morphologically distinct three species, S.

참고문헌 (29)

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