분포지역에 따른 민물가재 4집단(Eriocheir sinensis)의 지리적 변이 Geographic Variations in Four Freshwater Crab (Eriocheir sinensis) Populations throughout Its Distribution Range원문보기
Genomic DNA samples isolated from four geographical freshwater crab (Eriocheir sinensis) populations collected in the inland of the Korean Peninsula (Gunsan, Paju, and Nampo) and a Chinese site, were used for PCR amplification. Seven decamer primers generated 19 specific loci (19/243 loci, 7.81%) in...
Genomic DNA samples isolated from four geographical freshwater crab (Eriocheir sinensis) populations collected in the inland of the Korean Peninsula (Gunsan, Paju, and Nampo) and a Chinese site, were used for PCR amplification. Seven decamer primers generated 19 specific loci (19/243 loci, 7.81%) in the Gunsan population, 32 (32/215 loci, 14.88%) in the Paju population, 19 (19/231 loci, 8.23%) in the Nampo population and 62 (62/340 loci, 18.24%) in a Chinese population. The average 8.9 specific loci exhibited inter-individual-specific characteristics, thus revealing DNA polymorphisms in the Chinese population. The number of unique shared loci to each population and number of shared loci by the four populations were generated by molecular analysis using seven primers in four populations. 35 unique shared loci to each population, with an average of 5.0 per primer, were observed in the Gunsan population, and 50 loci, with an average of 7.1 per primer, were observed in the Chinese population. The hierarchical dendrogram indicates three main branches: cluster 1 (GUNSAN 01$\sim$GUNSAN 05, PAJU 06$\sim$PAJU 10 and NAMPO 11$\sim$NAMPO 15) and cluster 2 (CHINESE 16, 17, 18, 19 and 20). Conclusively individual no. 20 of the PAJU 10 freshwater crab was most distantly related to CHINESE no. 20 (genetic distance = 0.667). Taken together, these results demonstrate the potential of RAPD analysis to identify diagnostic markers for the identification of four freshwater crab populations.
Genomic DNA samples isolated from four geographical freshwater crab (Eriocheir sinensis) populations collected in the inland of the Korean Peninsula (Gunsan, Paju, and Nampo) and a Chinese site, were used for PCR amplification. Seven decamer primers generated 19 specific loci (19/243 loci, 7.81%) in the Gunsan population, 32 (32/215 loci, 14.88%) in the Paju population, 19 (19/231 loci, 8.23%) in the Nampo population and 62 (62/340 loci, 18.24%) in a Chinese population. The average 8.9 specific loci exhibited inter-individual-specific characteristics, thus revealing DNA polymorphisms in the Chinese population. The number of unique shared loci to each population and number of shared loci by the four populations were generated by molecular analysis using seven primers in four populations. 35 unique shared loci to each population, with an average of 5.0 per primer, were observed in the Gunsan population, and 50 loci, with an average of 7.1 per primer, were observed in the Chinese population. The hierarchical dendrogram indicates three main branches: cluster 1 (GUNSAN 01$\sim$GUNSAN 05, PAJU 06$\sim$PAJU 10 and NAMPO 11$\sim$NAMPO 15) and cluster 2 (CHINESE 16, 17, 18, 19 and 20). Conclusively individual no. 20 of the PAJU 10 freshwater crab was most distantly related to CHINESE no. 20 (genetic distance = 0.667). Taken together, these results demonstrate the potential of RAPD analysis to identify diagnostic markers for the identification of four freshwater crab populations.
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성능/효과
to 2, 500 bp, as shown in Fig. 1. In this study, on average, a decamer primer generated 34.7 amplifiE products per primer in the Gunsan population, and 48.6 in a Chinese population (Table 1). Seven decamer primers generated 19 specific loci (19/243 loci, 7.
The numter of imique shared loci to each population and number of shared loci by the four populations generated by RAPD analysis using 7 primers in Gunsan, Paju? Nampo and the Chinese populations. 35 unique sharedloci to each population, with an average of 5.0 per primer, were observed in the Gunsan population, and 50 loci, with an average of 7.1 per primer, were observed in the Chinese population (Table 2). Many researchers studied the sizes of DNA fragments in the PCR profiles of five species of Eastern Pacific abalone (genus Haliotis) (Muchmore et al.
참고문헌 (11)
Chenyambuga SW, Hanotte O, Hirbo J, Watts PC, Kemp SJ, Kifaro GC, Gwakisa PS, Petersen PH, Rege JEO (2004) Genetic characterization of indigenous goats of sub-Saharan Africa using microsatellite DNA markers. Asian-Aust J Anim Sci 17:445-452.
Koo JG, Jee JR, Kim 1M, Kang JC (2004) Effect of water temperature on survival, growth and intennolt period of tiger crab, Orithyia sinica (Linnaeus) larvae. J Fish Pathol 17:139-144.
McConnack GC, Powell R, Keegan B (2000) Comparative analysis of two populations of the brittle star Amphiura filiformis (Echinodennata: Ophiuroidae) with different life history strategies using RAPD markers. Mar Biotechnol 2:100-106.
Muchmore ME, Moy GW, Swanson WJ, Vacquier VD (1998) Direct sequencing of genomic DNA for characterization of a satellite DNA in five species of Eastern Pacific abalone. Mol Mar BioI Biotechnol 7:1-6.
Park SY, Park JS, Yoon JM (2005) Genetic differences and variations in slipper lobster (Ibacus ciliatus) and deep sea lobster (Puerulus sewelli) determined by RAPD analysis. Korean J Genet 25:307-317.
Tassanakajon A, Pongsomboon S, Jarayabhand P, Klinbunga S, Boonsaeng V (1998) Genetic structure in wild populations of black tiger shrimp (Penaeus monodon) using randomly amplified polymorphic DNA analysis. J Mar Biotech 6:249-254.
Yoke-Kqueen C, Radu S (2006) Random amplified polymorphic DNA analysis of genetically modified organisms. J Biotechnol 127:161-166.
Yoon JM, Kim GW (2003) Genetic differences between cultured and wild penaeid shrimp (Penaeus chinensis) populations analysed by RAPD-PCR. Korean J Genet 25:21-32.
Yoon JM, Kim JY (2004) Genetic differences within and between populations of Korean catfish (S. asotus) and bullhead (P. fulvidraco) analysed by RAPD-PCR. Asian-Aust J Anim Sci 17:1053-1061.
Yoon JM (2006) Genetic differences and variations in freshwater crab (Eriocheir sinensis) and swimming crab (Portunus trituberculatus). Dev Reprod 10(1):19-32.
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