[논문]녹나무 부탄올 분획물이 멜라닌 생합성에 미치는 영향

하상근; 문은정; 이민재; 박혜민; 유은숙; 오명숙; 김선여

[국내논문] 녹나무 부탄올 분획물이 멜라닌 생합성에 미치는 영향
Effect of the BuOH Soluble Fraction of Cinnamomum camphora on Melanin Biosynthesis 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.17 no.4, 2009년, pp.293 - 300

하상근 (경희대학교 동서의학대학원) , 문은정 (경희대학교 동서의학대학원) , 이민재 (경희대학교 동서의학대학원) , 박혜민 (경희대학교 동서의학대학원) , 유은숙 (제주대학교 의과대학) , 오명숙 (경희대학교 약학대학) , 김선여 (경희대학교 동서의학대학원)

Abstract ▼ AI-Helper

This study was carried out to investigate the effect of Cinnamomum camphora on melanogenesis. The MeOH extract of Cinnamomum camphora inhibited mushroom tyrosinase activity in dose-dependent manner. Moreover, it significantly suppressed the melanin production in melan-a cells at the concentration of $100{\mu}/m{\ell}$. The MeOH extract was partitioned with ethyl acetate, n-butanol and water. Among them, the BuOH soluble fraction exhibited significant inhibitory effect on mushroom tyrosinase. In addition, the BuOH soluble fraction reduced the melanin production in melan-a cells. But, the BuOH soluble fraction had less inhibition effects on melan-a cell originated tyrosinase. So, it was performed western blotting for melanogenic proteins (tyrosinase, tyrosinase-related protein (TRP-2)) using melan-a cells. The BuOH soluble fraction inhibited the protein expression of tyrosinase at the concentration of $100{\mu}/m{\ell}$. The results suggested that the BuOH soluble fraction of C. camphora might be a potent inhibitor of melanin biosynthesis in melan-a cells.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

This study was carried out to evaluate the protein levels of tyrosinase and TRP-2 on melan-a cells treated with the BuOH soluble fraction of Cinnamomum camphora. The BuOH soluble fraction affected the amount of tyrosinase protein (Fig.
To confirm the major compound of Cinnamomum camphor, quantitative analysis using GC was performed. The dried powdered leaves of Cinnamomum camphor extracts contained (+)-camphor (23.

대상 데이터

After removing the media on each well, the cells were washed with PBS. The 200 ㎕ of crystal violet (CV 0.1%, 10% EtOH, the rest is PBS) was added. It was incubated at room temperature for 5 min, and washed twice with water.

성능/효과

The camphor content of sample was analyzed by gas chromatography (GC). Identification of the constituent was based on comparison of the retention time with that of authentic camphor ((1R)-(+)-camphor, 98%). GC analysis was performed with a gas chromatography (GC-17A, Shimadzu, Japan) equipped with an omega wax 320 Fused Silica Capillary column (30 m × 0.
In conclusion, this study has demonstrated that the inhibitory activity of the BuOH soluble fraction of Cinnamomum camphora for melanin synthesis is mediated by decreasing expression of tyrosinase in melan-a cells. In addition, the BuOH soluble fraction is the most promising one to isolate the active depigmenting components.
In order to investigate more direct evidence that BuOH soluble fraction of Cinnamomum camphora inhibits melanogenesis, its effects on melanin production in melan-a cells were determined. Melanin synthesis was significantly inhibited at 100 ㎍/㎖ while the melanin content was reduced to 35% of that in non-treated cells at the same concentration (Fig.
7). Results showed that it did not inhibit tyrosinase enzyme activity but reduced tyrosinase protein expression. It is known that in addition to tyrosinase, there are other regulatory enzymes for melanin formation, such as TRP-2 (DT) and TRP-1.
The 95 wild plants from Cheju island originated natural resources were screened. This study showed that the extract of Cinnamomum camphora significantly inhibits mushroom tyrosinase activity.
In the present work, the methanol extract and the butanol soluble fraction of Cinnamomum camphora were investigated as a candidate material for inhibiting melanin biosynthesis in melan-a cells. This study shows that melanin production in melan-a cells treated with the BuOH soluble fraction decreases in a dose-dependant manner without cell toxicity by reducing the level of tyrosinase protein. These results further suggest that Cinnamomum camphora may be a candidate resource for skin lightening as well as for treating epidermal hyperpigmentation.

참고문헌 (19)

Aroca P, Urabe K, Kobayashi T, Tsukamoto K and Hearing VJ. (1993). Melanin biosynthesis patterns following hormonal stimulation. The Journal of Biological Chemistry. 268:25650-25655

상세보기
Bennett DC, Cooper PJ and Hart IR. (1987). A line of nontumorigenic mouse melanocytes, syngeneic with the B16 melanoma and requiring a tumour promoter for growth. International Journal of Cancer. 39:414-418

상세보기
Briganti S, Camera E and Picardo M. (2003). Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell Research. 16:101-110

상세보기
Curto EV, Kwong C, Hermersdorfer H, Glatt H, Santis C, Virador V, Hearing VJ Jr. and Dooley TP. (1999). Inhibitors of mammalian melanocyte tyrosinase: in vitro comparisons of alkyl esters of gentisic acid with other putative inhibitors. Biochemical Pharmacology. 57:663-672

상세보기
del Marmol V and Beermann F. (1996). Tyrosinase and related proteins in mammalian pigmentation. FEBS Letters. 381:165-168

상세보기
Fuller BB, Drake MA, Spaulding DT and Chaudhry F. (2000). Downregulation of tyrosinase activity in human melanocyte cell cultures by yohimbine. Journal of Investigative Dermatology. 114:268-276

상세보기
Hearing VJ and Tsukamoto K. (1991). Enzymatic control of pigmentation in mammals. FASEB Journal. 5:2902-2909

상세보기
Hosoi J, Abe E, Suda T and Kuroki T. (1985). Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Research 45:1474-1478

상세보기
Jeong HS, Han JG, Ha JH, Kim Y, Oh SH, Kim SS, Jeong MH, Choi GP, Park UY and Lee HY. (2009). Antioxidant activities and skin-whitening effects of nano-encapsuled water extract from Rubus coreanus Miquel. Korean Journal of Medicinal Crop Science. 17:83-89
Jo YO, Kong YH, Lee YC, Kim SS and Choi SY. (2008). Inhibitory effect of white ginseng fraction on skin pigmentation. Korean Journal of Medicinal Crop Science. 16:192-194
Kobayashi T, Urabe K, Winder A, Jimenez-Cervantes C, Imokawa G, Brewington T, Solano F, Garcia-Borron JC and Hearing VJ. (1994). Tyrosinase related protein 1 (TRP1) functions as a DHICA oxidase in melanin biosynthesis. EMBO Journal. 13:5818-5825
Maeda K, Yokokawa Y, Hatao M, Naganuma M and Tomita Y. (1997). Comparison of the melanogenesis in human black and light brown melanocytes. Journal of Dermatological Science. 14:199-206

상세보기
Mukherjee RK, Fujimoto Y and Kakinuma K. (1994). 1-( $\omega$ - Hydroxyfattyacyl)glycerols and two flavanols from Cinnamomum camphora . Phytochemistry. 37:1641-1643

상세보기
Ozeki H, Ito S, Wakamatsu K and Thody AJ. (1996). Spectrophotometric characterization of eumelanin and pheomelanin in hair. Pigment Cell Research. 9:265-270

상세보기
Prota G. (1993). Regulatory mechanisms of melanogenesis: beyond the tyrosinase concept. The Journal of Investigative Dermatology. 100:156S-161S

상세보기
Shono S and Toda K. (1981). Pigment Cell. University of Tokyo Press, Tokyo, pp. 263-268
Sulaimon SS and Kitchell BE. (2003). The biology of melanocytes. Veterinary Dermatology. 14:57-65

상세보기
Takaoka D and Hiroi M. (1976). Two acyclic monoterpene diols from Cinnamomum camphorha. Phytochemistry. 15:330

상세보기
Takaoka D, Imooka M and Hiroi M. (1979). A novel cyclopentenone, 5-dodecanyl-4-hydroxy-4-methyl-2-cyclopentenone from Cinnamomum camphora. Phytochemistry. 18:488-489

상세보기

저자의 다른 논문 :

LOADING...

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증