[논문]미니돼지에서 University of Wisconsin 용액과 Histidine-Tryptophan Ketoglutarate 용액의 신장 저온보존효과 비교

남현숙; 윤병일; 우흥명

미니돼지에서 University of Wisconsin 용액과 Histidine-Tryptophan Ketoglutarate 용액의 신장 저온보존효과 비교
Comparison of the Effectiveness of University of Wisconsin and Histidine-Tryptophan Ketoglutarate Solution on Renal Cold Preservation in the Miniature Pig 원문보기

Journal of veterinary clinics = 한국임상수의학회지, v.26 no.5 = no.70, 2009년, pp.445 - 449

남현숙 (강원대학교 수의(학부) 대학) , 윤병일 (강원대학교 수의(학부) 대학) , 우흥명 (강원대학교 수의(학부) 대학)

초록
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이식분야에서 University of Wisconsin (UW)용액과 Histidine-Tryptophan Ketoglutarate (HTK)용액은 장기 보존액으로 주로 이용되고 있다. 바이오 장기 동물모델인 미니돼지를 이용하여 신장 저온보존에서 발생하는 허혈손상을 비교함으로써 각 보존액의 효용성을 검토하고자 하였다. 이 연구에서는 12마리의 미니돼지가 이용되었으며, 각 6마리씩 무작위로 선별하여 UW군(n = 6)과 HTK군(n = 6) 두 군으로 나누었다. 미니돼지의 신장을 적출, 세척, 각각 저온 보관(0, 24, 48, 72시간, $4^{\circ}C$) 후에 조직학적 평가를 실시하였다. 저온보관시간이 증가할수록 신장의 손상이 증가하였다. 저온보관 24시간까지는 UW와 HTK용액의 저온보관 효과는 유사하였지만, 48시간 이후에는 HTK 용액의 저온보관손상이 증가하였다. 따라서, 미니돼지 신장의 저온 보존액은 UW 용액이 효과적이며 특히 장기간 보존에 적합한 용액으로 사료된다.

Abstract ▼ AI-Helper

For human organ transplantations, histidine-tryptophan-ketoglutarate solution (HTKS) and University of Wisconsin solution (UWS) have been shown to engender similar outcomes as gold standard cold preservation solutions ($4^{\circ}C$). To select the effective preservation solution for cold storage of kidney xenografts in miniature pig, which could be a potential source animal of bio-organs, this study compared early histopathological outcomes of cold preservation injury using HTKS and UWS. Twelve miniature pigs weighing 25.6 to 34.7 kg were divided into two groups (n = 6 per group), UWS group and HTKS group. The kidneys in each group were harvested, cold flushed, and preserved for 0, 24, 48, and 72 hrs at $4^{\circ}C$ with UWS or HTKS, respectively. Histolopathological examinations were assessed on kidney biopsy specimens, taken after each cold storage. The degree of renal injury was scored using 5 different criteria (pyknotic nuclei, disruption of cytoplasm, detachment of epithelium, loss of microvilli, tubular necrosis and loss of glomerular tufts) of the cellular components of the tissue. The degree of kidney damage was increased with prolonged cold ischemia time. UWS and HTKS have at least similar efficacy in kidney preservation within 24 hrs cold preservation time. However, in HTKS group cold-induced injury started to be observed more than in UWS group after 48 hrs of cold storage. In conclusion, UWS and HTKS were equally effective for cold preservation of miniature pig kidney in early preservation times; however, UWS may be more effective at longer preservation times as compared to HTKS.

주제어

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문제 정의

UWS and HTKS have been used to decrease cold ischemic kidney injury in experimental pig models (9,13) The aim of this study was to compare the kidney preservation efficacy of UWS with HTKS in miniature-pig, which is going to be a source animal of bio-organs.

제안 방법

Histopathological examination was performed in the kidneys respectively stored in the UWS and HTKS preservation solutions at 4°C for 0, 24, 48 and 72 hours.
Fig 1. The kidneys stored in the cold HTKS (A to D), UWS (E to H) for 24 hours (B and F), 48 hours (C and G) and 72 hours (D and H), respectively. Renal injury was typically observed 48 and 72 hours after cold storage in HTKS but such morphological changes were considerably ameliorated in UW solution.
Fig 2. The kidneys stored in the cold HTKS (A to D), UWS (E to H) for 24 hours (B, F), 48 hours (C, G) and 72 hours (D, H) respectively. Note the basement membranes and microvilli stained with pink color by PAS staining.

대상 데이터

Twelve miniature-pigs weighing 25.6 to 34.7 kg (PWG® Prestige) were used.

성능/효과

In conclusion, the present study shows that cytoprotection during cold ischemia by UWS preservation may lead to the limitation of cold ischemia injury in the miniature pig kidney. We also show that preservation of kidneys with HTKS is equivalent to UWS, except after 48 hr and 72hr of cold storage.

후속연구

We also show that preservation of kidneys with HTKS is equivalent to UWS, except after 48 hr and 72hr of cold storage. Further experimental studies are necessary to assess the damage in an ischemia and reperfusion model including the additional reperfusion injury.

참고문헌 (22)

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