White rot, which is caused by Sclerotium cepivorum, is a lethal disease affecting green onions. Three different types of nanosilver liquid (WA-CV-WA13B, WA-AT-WB13R, and WA-PR-WB13R) were tested in several different concentrations on three types of media to assess their antifungal activities. Result...
White rot, which is caused by Sclerotium cepivorum, is a lethal disease affecting green onions. Three different types of nanosilver liquid (WA-CV-WA13B, WA-AT-WB13R, and WA-PR-WB13R) were tested in several different concentrations on three types of media to assess their antifungal activities. Results from in vitro experiments showed that all three of the nano-silver liquids had more than 90% inhibition rates at a concentration of 7 ppm. Greenhouse experiments revealed that all of the nano-silver liquids increased biomass and dry weights, and there were minimal changes in the population of various bacteria and fungi from the soil of greenhouse-cultivated green onions. In addition, a soil chemical analysis showed that there were minimal changes in soil composition.
White rot, which is caused by Sclerotium cepivorum, is a lethal disease affecting green onions. Three different types of nanosilver liquid (WA-CV-WA13B, WA-AT-WB13R, and WA-PR-WB13R) were tested in several different concentrations on three types of media to assess their antifungal activities. Results from in vitro experiments showed that all three of the nano-silver liquids had more than 90% inhibition rates at a concentration of 7 ppm. Greenhouse experiments revealed that all of the nano-silver liquids increased biomass and dry weights, and there were minimal changes in the population of various bacteria and fungi from the soil of greenhouse-cultivated green onions. In addition, a soil chemical analysis showed that there were minimal changes in soil composition.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
문제 정의
Changes in soil microbe populations: We investigated the soil fungi and bacteria by comparing the microorganism population in soil samples taken at the places where nano-silver liquid was used to treat green onions. This experiment was conducted to determine if nano-silver treatment affected useful microbes (Tables 5 and 6). The number of bacteria in the soil from treated individuals generally decreased in comparison to the soil from nontreated individuals.
This research has shown that nano-silver is harmless in the human body if safety measures are applied and that nano-silver liquid can increase plant productivity and reduce environmental pollution.
제안 방법
Among them Sclerotium cepivorum is the most prevalent. Therefore, in this study we attempted to control the disease caused by this fungi using nano-silver liquid. These results suggest that nano-silver is effective for fungi growth inhibition.
Antifungal effects of nano-silver liquid: in vitro and field tests. To determine the antifungal activities of nano-silver against S. cepivorum, three different types of nano-silver liquid with its four mixtures in eight different concentrations (1 ppm, 3 ppm, 5 ppm, 7 ppm, 10 ppm, 25 ppm, 50 ppm, 100 ppm) were tested. A mycelial growth inhibition rate was determined by using the following formula.
Field tests. We applied different concentrations of nanosilver (10 ppm, 23 ppm, 50 ppm, and 100 ppm) at the base of each plant 4 times each week for up to 4 weeks. After 5 months, the roots of the plants were observed and the dry weight was measured after drying it in drying machine at 80oC.
대상 데이터
Diseased green onions were removed from a farmhouse in Yangsu-ri, Gyungki-do and the growth of a mycelium was observed. The three different nano-silver liquids were each used at a concentration of 1 ppm, 3 ppm, 5 ppm, 7 ppm, 10 ppm, 25 ppm, 50 ppm, and 100 ppm on PDA, MEA and CMA culture plates (Fig. 1).
Nano-silver liquid. Three types of nano-silver liquid WA-CV-WA13B, WA-AT-WB13R, and WA-PR-WB13R (Table 1) were provided by the Bio-plus Co. (Pohang, Korea) at a 1,000 ppm initial concentration that was then diluted into different working concentrations.
성능/효과
The inhibition rate was 86% on PDA with a 5 ppm concentration and 93% on CMA with a 3 ppm concentration. An inhibition rate of 81% of the control percentage on an MEA plate at a 25 ppm concentration on nano-silver WAPR- WB13R, 81% inhibition was recorded on PDA at a 7 ppm concentration, 84% on CMA with a 10 ppm concentration, and 81% on MEA at a 25 ppm concentration. Over all, nano-silver had the best inhibitory effect on PDA.
To analyze the changes in the microbe population in the soil where green onions were collected and treated with or without nano-silver liquid. The bacterial population was decreased more in the treated soil in comparison the non-treated soil, and fungi populations other than S. cepivorum, which was not found, increased. In the early stage bacteria decreased but later on it increased.
참고문헌 (16)
1 Coley-Smith JR White rot disease of Allium: problems of soil-borne diseases in microcosm Plant Pathol 1990 39 214 222
2 Abd El-Razik AA Shatla MN Rushdi M Studies on the infection of onion plants by Sclerotium cepivorum Berk Phytopathologische Zeitschrift 1973 76 108 116
3 Entwistle AR Allium white rot and its control Soil Use Manag 1990 6 201 209
4 Coley-Smith JR Mitchell CM Sansford CE Long-term survival of sclerotia of Sclerotium cepivorum and Stromatinia gladioli Plant Pathol 1990 39 58 69
5 Coley-Smith JR Chet I Alternative methods of controlling white rot disease of Allium Innovative approaches to plant disease control 1987 New York Wiley 161 177
6 Cho WD Kim WG Hong SK Kim WS Sclerotium sp. associated with occurrence of a new disease, globular Sclerotium rot of garlic Plant Pathol J 2003 18 372
7 Cho WD Kim WG Sclerotium sp. occurrence in the vegetable crops of species of Allium Plant Pathol J 1996 12 251 254
8 Crowe FJ Hall DH Soil temperature and moisture effects on Sclerotium germination and infection of onion seedlings by Sclerotium cepivorum Phytopathology 1980 70 74 78
9 Mordue JE Sclerotium cepivorum . CMI descriptions of pathogenic fungi and bacteria, No. 512 1976 Kew Commonwealth Mycological Institute
10 Kang HY Jung MJ Jeong YK Antibacterial activity and the stability of an Ag + solution made using metallic silver Korean J Biotechnol Bioeng 2000 15 521 524
11 Lim YT Choi HK Ahn YH Antimicrobial activity assessment of functional plastics which contained Ag-Hydroxyl Apatite agent Korean J Biotechnol Bioeng 2001 16 123 127
12 Cho KH Park SG Study on the antibacterial effects of silver nanoparticles prepared by sonication method J Korean Ind Eng Chem 2004 15 952 955
13 Bragg PD Rainnie DJ The effect of silver ions on the respiratory chain of Escherichia coli Can J Microbiol 1974 20 883 889 4151872
14 Feng QL Wu J Chen GQ Cui FZ Kim TN Kim JO A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus J Biomed Mater Res 2000 52 662 668 11033548
15 Kim JH Nano silver chemotherapeutic agents and its applications NICE 2004 22 656 660
16 Morones JR Elechiguerra JL Camacho A Holt K Kouri JB Ramírez JT The bactericidal effect of silver nanoparticles Nanotechnology 2005 16 2346 2353 20818017
※ AI-Helper는 부적절한 답변을 할 수 있습니다.