To establish the anti-inflammatory activity of the total flavonoid fraction of the root barks of Broussonetia papyrifera (EBP) and a new formula, the ethanol extract of the root barks of B. papyrifera was fractionated with ethylacetate, yielding the hydrophobic prenylated flavonoid-enriched fraction...
To establish the anti-inflammatory activity of the total flavonoid fraction of the root barks of Broussonetia papyrifera (EBP) and a new formula, the ethanol extract of the root barks of B. papyrifera was fractionated with ethylacetate, yielding the hydrophobic prenylated flavonoid-enriched fraction. EBP and the ethanol extract of the whole Lonicera japonica (ELJ) plant were then mixed at a ratio of 1:1 (w/w) to give a new preparation (BL) in the hope of obtaining an optimal formula with a higher anti-inflammatory activity. Evaluation of the effects of these preparations on A23187-treated rat basophilic leukemia (RBL-1) cells revealed that EBP potently inhibited 5-lipoxygenase (5-LOX), while ELJ showed weak inhibition. Additionally, the mixture (BL) clearly showed stronger inhibitory effects against 5-LOX than either preparation alone. These preparations also inhibited cyclooxygenase-2-catalyzed $PGE_2$ and inducible nitric oxide (NO) synthase-catalyzed NO production by lipopolysaccharide-treated RAW 264.7 cells. When tested against arachidonic acid-induced mouse ear edema, EBP showed strong inhibitory activity at doses of 5-200 mg/kg when administered orally, but BL had obviously stronger inhibitory effects. When tested against ${\lambda}$-carrageenan-induced paw edema in mice, BL showed a potent and synergistic anti-inflammatory effect. In addition, in the acetic acid-induced writhing test, BL was found to have strong analgesic activity at 50-400 mg/kg. Taken together, these results indicate that each of these preparations exert anti-inflammatory activity in vitro and in vivo. In particular, BL showed stronger anti-inflammatory activity than EBP, and these anti-inflammatory effects were partially related to the inhibition of eicosanoid and NO production. BL may be useful for the treatment of human inflammatory disorders.
To establish the anti-inflammatory activity of the total flavonoid fraction of the root barks of Broussonetia papyrifera (EBP) and a new formula, the ethanol extract of the root barks of B. papyrifera was fractionated with ethylacetate, yielding the hydrophobic prenylated flavonoid-enriched fraction. EBP and the ethanol extract of the whole Lonicera japonica (ELJ) plant were then mixed at a ratio of 1:1 (w/w) to give a new preparation (BL) in the hope of obtaining an optimal formula with a higher anti-inflammatory activity. Evaluation of the effects of these preparations on A23187-treated rat basophilic leukemia (RBL-1) cells revealed that EBP potently inhibited 5-lipoxygenase (5-LOX), while ELJ showed weak inhibition. Additionally, the mixture (BL) clearly showed stronger inhibitory effects against 5-LOX than either preparation alone. These preparations also inhibited cyclooxygenase-2-catalyzed $PGE_2$ and inducible nitric oxide (NO) synthase-catalyzed NO production by lipopolysaccharide-treated RAW 264.7 cells. When tested against arachidonic acid-induced mouse ear edema, EBP showed strong inhibitory activity at doses of 5-200 mg/kg when administered orally, but BL had obviously stronger inhibitory effects. When tested against ${\lambda}$-carrageenan-induced paw edema in mice, BL showed a potent and synergistic anti-inflammatory effect. In addition, in the acetic acid-induced writhing test, BL was found to have strong analgesic activity at 50-400 mg/kg. Taken together, these results indicate that each of these preparations exert anti-inflammatory activity in vitro and in vivo. In particular, BL showed stronger anti-inflammatory activity than EBP, and these anti-inflammatory effects were partially related to the inhibition of eicosanoid and NO production. BL may be useful for the treatment of human inflammatory disorders.
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문제 정의
Although these previous results also demonstrated that, among the extracts, the radix showed the most comparable activity, our preparations (EBP and BL) had much higher in vivo anti-inflammatory activities at 50-400 mg/kg. Thus, these findings may support the research rational of our study to prepare flavonoid-enriched fraction of the radix of B. papyrifera.
제안 방법
, 2003b). Because 5-LOX inhibitors have the potential for use in the treatment of inflammatory disorders such as bronchial asthma and bronchitis, and because iNOS inhibitors may also be used as anti-inflammatory agents, the prenylated flavonoid-enriched fraction from the root barks of B. papyrifera (EBP) was prepared and its effects on several inflammatory models were examined in this study.
chow and water ad libitum. The animals were maintained in the animal facility (KNU) at 20-22℃ under 40-60% relative humidity and a 12 h/12 h (light/dark) cycle for at least 7 days prior to the experiment. The experimental design using the animals was approved by the local committee for animal experimentation, KNU (KIACUC-09-0012).
대상 데이터
, 2001). The whole plant of L. japonica was purchased from a local herbal market, authenticated by Dr. K. H. Son and a voucher specimen was deposited in Andong National University. The dried plant was then chopped and extracted with 70% ethanol.
데이터처리
One-way analysis of variance (ANOVA), followed by Dunnett’s test was used to determine the statistical significance.
성능/효과
In conclusion, EBP, ELJ and BL were found to exert significant anti-inflammatory activity in vitro and in vivo. Especially, BL showed potent and enhancing effects in several animal models of inflammation.
후속연구
To develop a new anti-inflammatory herbal drug, the ethanol extract (ELJ) was examined for its in vitro and in vivo activity in animal models of inflammation. In addition, a new formulation (BL) composed of EBP and ELJ (1:1, w/w) was prepared and its anti-inflammatory activity was examined in the present investigation with the hope of obtaining a stronger anti-inflammatory effect.
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